Virological Investigations of Herpesvirus saimiri-lnSected
Owl Monkeys

Neubauer, R H; Wallen, William C.; Rabin, Harvey; Pearson, G. R.; Ablashi, D. V.

doi:10.1159/000459962

Cited by 9 publications

(3 citation statements)

References 21 publications

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“…saimiri assays were performed, in quadruplicate, in 16-mm-well culture plates (Linbro Chemical Co., Waltham, Mass.) as described (11). Herpes simplex virus, type 1 (obtained from J. Docherty, Pennsylvania State University, State College, Pa.), was assayed in 35-mm-well culture plates (Linbro) using overlay media containing 1% human serum.…”

Section: Methodsmentioning

confidence: 99%

Inhibition of the mitogenic response of normal peripheral lymphocytes by extracts or supernatant fluids of a Herpesvirus saimiri lymphoid tumor cell line

Neubauer¹,

Wallen²,

Rabin³

1975

Infect Immun

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A Herpesvirus saimiri-infected marmoset lymphoid cell line (MLC-1) was examined for the presence of soluble factors which might affect lymphocyte functions and, therefore, relate to the pathogenesis of lymphoma in vivo. MLC-1 cells, cell extracts, and culture fluids were shown to reduce the spontaneous deoxyribonucleic acid (DNA) synthesis of normal peripheral blood lymphocytes and to completely inhibit their response to phytohemagglutinin (PHA). Suppression of PHA response was demonstrated against a variety of human and nonhuman primate species, with 90 to 95% inhibition occurring at dilutions of extract as great as 1:5,120. Inhibition of this type was also demonstrated using extracts of two of five other lymphoblastoid cell lines tested. Physical-chemical characteristics of the active factor(s) revealed a non-sedimentable, non-dialyzable, trypsin-resistant molecule, which was stable at 56 C for 30 min but inactivated at 80 C for 30 min. The factor(s) also exerted an effect on some but not all established lymphoblastoid cell lines, where DNA, ribonucleic acid, and protein synthesis were all inhibited, with DNA synthesis being the most affected (95% suppression). Cellular respiration was not affected by the presence of the factor(s), and the inhibition of DNA synthesis was reversible after 24 h. Purified human interferon did not reduce the PHA response of normal owl monkey periperal blood lymphocytes and was less effective against an established lymphoblastoid cell line than the MLC-1 extract. Antiviral activity was also demonstrated in the preparations and may represent interferon, which these cells are known to produce at low levels.

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Section: Methodsmentioning

confidence: 99%

Inhibition of the mitogenic response of normal peripheral lymphocytes by extracts or supernatant fluids of a Herpesvirus saimiri lymphoid tumor cell line

Neubauer¹,

Wallen²,

Rabin³

1975

Infect Immun

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“…The first hypothesis may be supported by the fact that all attempts to isolate cell-free virus from serum and oral swabs of tumordeveloping owl monkeys and marmosets remained unsuccessful Neubauer et al, 1974c). Herpesvirus saimiriinfected marmosets and owl monkeys do not excrete appreciable amounts of infectious virus.…”

Section: A Permissive and Transforming Infection In Tumor-developingmentioning

confidence: 99%

The Herpesviruses

1982

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“…Although the oncogenicity of these viruses has not been demonstrated, the inoculation of both of them into day-old guinea pigs resulted in the enlargement of spleen and lymph nodes (18). In HVS studies, the examination of viral inocula as well as tumor and other tissues from owl monkeys and marmosets with lymphomas has not revealed evidence for the presence of type C viruses (8,26,27). RNAdependent DNA (12,14).…”

mentioning

confidence: 99%

Stimulation of Herpesvirus saimiri Expression in the Absence of Evidence for Type C Virus Activation in a Marmoset Lymphoid Cell Line

Neubauer

Wallen

Rabin

1974

J Virol

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Nucleic acid base analogues were used to examine a Herpesvirus saimiri (HVS)-infected marmoset lymphoid cell line (MLC-1) for possible association with type C viruses. Synthetic templates poly(rA) * d(pT),10 and poly(dA) d(pT)1,0 were used to detect RNA-directed DNA polymerase activity in 100-fold concentrated tissue culture fluids. HVS was monitored by immunofluorescence for early, late, and membrane antigens. MLC-1 cells were exposed to 30 ,ug of 5-bromo-2'-deoxyuridine (BUdR) per ml for 24 h and examined daily. Similar experiments used 5-iodo-2'-deoxyuridine (IUdR) (20 ,g/ml) for 30 h or IUdR (20

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Virological Investigations of Herpesvirus saimiri-lnSected Owl Monkeys

Cited by 9 publications

References 21 publications

Inhibition of the mitogenic response of normal peripheral lymphocytes by extracts or supernatant fluids of a Herpesvirus saimiri lymphoid tumor cell line

Inhibition of the mitogenic response of normal peripheral lymphocytes by extracts or supernatant fluids of a Herpesvirus saimiri lymphoid tumor cell line

The Herpesviruses

Stimulation of Herpesvirus saimiri Expression in the Absence of Evidence for Type C Virus Activation in a Marmoset Lymphoid Cell Line

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