Virulence and antimicrobial resistance genes are enriched in the plasmidome of clinical Escherichia coli isolates compared with wastewater isolates from western Kenya

Wawire, Sifuna Anthony; Reva, Oleg N.; O'Brien, Thomas J; Figueroa, Wendy; Dinda, Victor; Shivoga, W. A.; Welch, Martin

doi:10.1016/j.meegid.2021.104784

Cited by 5 publications

(4 citation statements)

References 38 publications

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“…The serotypes were H18:O17 for E. coli 3/145 and H5:O75 for E. coli 19/278. ST3 isolates are common causative agents of colitis, diarrhea and urinary infections [ 13 ]. According to the MLST database records, only a few cases of acute colitis reported in Iraq were associated with ST53.…”

Section: Resultsmentioning

confidence: 99%

“…As to the P. aeruginosa and the Gram-positive isolates, there were less than 10% of the virulence genes associated with mobilomes, and none of them was in S. pneumoniae PHRX1-2021. Attention was paid to the distribution of antibiotic resistance and virulence genes between the core and mobile parts of the genomes due to a hypothesis stating that mobilome-associated genes may have a greater effect on the phenotype than the genes located in the core chromosomal regions [ 13 ].…”

Section: Discussionmentioning

confidence: 99%

“…This is why MLST and serotyping are popular techniques aiding in the estimation of the epidemiological risks of discovered pathogenic bacterial isolates. For instance, outbreaks of intrahospital enterobacterial infections in the Eurasian region are most often associated with Escherichia coli ST43 and ST131, whereas several other STs of E. coli cause hospital infection in other geographical regions around the globe [ 13 ]. Hospital-acquired infections caused by various pathogens are frequent in Central Asian hospitals, particularly in Kazakhstan.…”

Section: Introductionmentioning

confidence: 99%

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Analysis of Whole-Genome Sequences of Pathogenic Gram-Positive and Gram-Negative Isolates from the Same Hospital Environment to Investigate Common Evolutionary Trends Associated with Horizontal Gene Exchange, Mutations and DNA Methylation Patterning

Korotetskiy¹,

Shilov²,

Kuznetsova³

et al. 2023

Microorganisms

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Hospital-acquired infections are a generally recognized problem for healthcare professionals. Clinical variants of Gram-negative and Gram-positive pathogens are characterized with enhanced antibiotic resistance and virulence due to mutations and the horizontal acquisition of respective genetic determinants. In this study, two Escherichia coli, two Klebsiella pneumoniae, three Pseudomonas aeruginosa, two Staphylococcus aureus, one Staphylococcus epidermidis and one Streptococcus pneumoniae showing broad spectra of antibiotic resistance were isolated from patients suffering from nosocomial infections in a local hospital in Almaty, Kazakhstan. The aim of the study was to compare general and species-specific pathways of the development of virulence and antibiotic resistance through opportunistic pathogens causing hospital-acquired infections. The whole-genome PacBio sequencing of the isolates allowed for the genotyping and identification of antibiotic resistance and virulence genetic determinants located in the chromosomes, plasmids and genomic islands. It was concluded that long-read sequencing is a useful tool for monitoring the epidemiological situation in hospitals. Marker antibiotic resistance mutations common for different microorganisms were identified, which were acquired due to antibiotic-selective pressure in the same clinical environment. The genotyping and identification of strain-specific DNA methylation motifs were found to be promising in estimating the risks associated with hospital infection outbreaks and monitoring the distribution and evolution of nosocomial pathogens.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Analysis of Whole-Genome Sequences of Pathogenic Gram-Positive and Gram-Negative Isolates from the Same Hospital Environment to Investigate Common Evolutionary Trends Associated with Horizontal Gene Exchange, Mutations and DNA Methylation Patterning

Korotetskiy¹,

Shilov²,

Kuznetsova³

et al. 2023

Microorganisms

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“…The E. coli isolates belonged to MLST ST43 and ST3, which are widely distributed uropathogens ( 9 ). K. pneumoniae ST23 and ST380 are abundant hypervirulent and multidrug-resistant variants which emerged due to the acquisition of pLVPK-type virulence plasmids ( 10 – 12 ).…”

Section: Announcementmentioning

confidence: 99%

Complete Genome Sequences of Gram-Negative Opportunistic Pathogens Isolated in Hospitals in Almaty, Kazakhstan

Korotetskiy¹,

Jumagaziyeva²,

Kerimzhanova³

et al. 2021

Microbiol Resour Announc

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The problem of nosocomial infections is growing due to the introduction of new treatment regimens involving immunosuppressive drugs. The genomes of seven Gram-negative clinical isolates of Escherichia , Klebsiella , and Pseudomonas were sequenced and analyzed in this study to serve as model microorganisms to study drug-induced antibiotic resistance reversion.

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Biodegradation mechanism of chloramphenicol by Aeromonas media SZW3 and genome analysis

Tan

Yang

Chen

et al. 2022

Bioresource Technology

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Virulence and antimicrobial resistance genes are enriched in the plasmidome of clinical Escherichia coli isolates compared with wastewater isolates from western Kenya

Cited by 5 publications

References 38 publications

Analysis of Whole-Genome Sequences of Pathogenic Gram-Positive and Gram-Negative Isolates from the Same Hospital Environment to Investigate Common Evolutionary Trends Associated with Horizontal Gene Exchange, Mutations and DNA Methylation Patterning

Analysis of Whole-Genome Sequences of Pathogenic Gram-Positive and Gram-Negative Isolates from the Same Hospital Environment to Investigate Common Evolutionary Trends Associated with Horizontal Gene Exchange, Mutations and DNA Methylation Patterning

Complete Genome Sequences of Gram-Negative Opportunistic Pathogens Isolated in Hospitals in Almaty, Kazakhstan

Biodegradation mechanism of chloramphenicol by Aeromonas media SZW3 and genome analysis

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