Virulence and Enzyme Production of <i>Erwinia carotovora</i> ssp. <i>atroseptica</i> on Potato Tuber Tissue

Weber, Jürgen; Wegener, Christina

doi:10.1111/j.1439-0434.1986.tb00633.x

Cited by 11 publications

(2 citation statements)

References 9 publications

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“…Many authors have reported that potato slices inoculated by Pectobacterium strains showed soft rot symptoms resulting of the secretion of various extracellular enzymes (Bain and Pérombelon 1988;Karlovsky et al 2008;Lapwood and Read 1985;Lapwood et al 1984;Pérombelon 2002;Terta et al 2010;Toth et al 2003;Weber and Wegener 1986), and that the pathogenicity of P. carotovorum and P. atrosepticum is known to be regulated by quorum sensing; a population density-dependent modulation of the bacterial phenotype (Barnard et al 2007;Swift et al 1996). The disease could be efficiently inhibited by biocontrol agents maintaining the pathogen population below the level required for symptom development.…”

Section: Discussionmentioning

confidence: 97%

Control of potato soft rot caused by Pectobacterium carotovorum and Pectobacterium atrosepticum by Moroccan actinobacteria isolates

Baz

Lahbabi

Samri

et al. 2011

World J Microbiol Biotechnol

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Pectobacterium carotovorum and Pectobacterium atrosepticum are dreadful causal agents of potato soft rot. Actually, there are no efficient bactericides used to protect potato against Pectobacterium spp. Biological control using actinobacteria could be an interesting approach to manage this disease. Thus, two hundred actinobacteria isolated from Moroccan habitats were tested for their ability to inhibit in vitro 4 environmental Pectobacterium strains and the two reference strains (P. carotovorum CFBP 5890 and P. atrosepticum CFBP 5889). Eight percent of these isolates were active against at least one of the tested pathogens and only 2% exhibited an antimicrobial activity against all tested Pectobacterium strains. Four bioactive isolates having the greatest pathogen inhibitory capabilities and classified as belonging to the genus Streptomyces species through 16S rDNA analysis were subsequently tested for their ability to reduce in vivo soft rot symptoms on potato slices of Bintje, Yukon Gold, Russet and Norland cultivars caused by the two pathogens P. carotovorum and P. atrosepticum. This test was carried out by using biomass inoculums and culture filtrate of the isolates as treatment. Among these, strain Streptomyces sp. OE7, reduced by 65-94% symptom severity caused by the two pathogens on potato slices. Streptomyces OE7 showed a potential for controlling soft rot on potato slices and could be useful in an integrated control program against potato soft rot pathogens in the objective to reduce treatments with chemical compounds.

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Section: Discussionmentioning

confidence: 97%

Control of potato soft rot caused by Pectobacterium carotovorum and Pectobacterium atrosepticum by Moroccan actinobacteria isolates

Baz

Lahbabi

Samri

et al. 2011

World J Microbiol Biotechnol

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show abstract

“…A successful defence of invading Erwinia carotovora (Ec) bacteria needs an early recognition of the pathogen by the host plant (Weber & Wegener, 1986). Unsaturated oligogalacturonates (OG) released from plant cell wall pectin by the action of pectate lyase (PL) enzymes, the major virulence determinant of Erwinia carotovora bacteria, elicit plant defence responses (Ryan, 1988).…”

Section: Introductionmentioning

confidence: 99%

Transgenic potatoes expressing anErwinia pectate lyase gene — results of a 4-year field experiment

Wegener

2001

Potato Res

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SummaryFour potato lines of cv. D6sir6e that express the pectate lyase (PL) isoenzyme 3 of Erwinia carotovora subsp, atroseptica were examined in a 4-year field experiment with respect to plant development, tuber yield and resistance of tuber tissue to Erwinia soft rot. The PL3 degrades plant cell wall pectin into unsaturated oligogalacturonates eliciting plant defence responses. In one line, enzyme expression was controlled by the CaMV 35S promoter (C) and in three lines it was driven by the potato patatin B33 promoter (D). Plant development of the D-lines in field plots was not distinguishable from that of the non-transgenic counterpart. Also tuber yield was not too different. By contrast, plants of the C-line were smaller than those of "the nontransformed counterpart and also showed reduced tuber yield. There were no significant differences in dry mass. starch and protein content of tuber tissue between PL transgenic and non-transgenic potatoes. But compared with the latter, field-grown tubers expressing the PL displayed an enhanced resistance to Erwinia soft rot. Thus, average rotting caused by Ecbacteria was diminished in tubers of PL-transgenic lines by 34.1%. The resistance of tubers to Ec soft rot was significantly correlated with the PPO activity in tuber tissue.

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Induction of defence responses against Erwinia soft rot by an endogenous pectate lyase in potatoes

Wegener

2002

Physiological and Molecular Plant Pathology

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Virulence and Enzyme Production of Erwinia carotovora ssp. atroseptica on Potato Tuber Tissue

Cited by 11 publications

References 9 publications

Control of potato soft rot caused by Pectobacterium carotovorum and Pectobacterium atrosepticum by Moroccan actinobacteria isolates

Control of potato soft rot caused by Pectobacterium carotovorum and Pectobacterium atrosepticum by Moroccan actinobacteria isolates

Transgenic potatoes expressing anErwinia pectate lyase gene — results of a 4-year field experiment

Induction of defence responses against Erwinia soft rot by an endogenous pectate lyase in potatoes

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