2023
DOI: 10.1101/2023.01.12.523823
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Virulence and genomic diversity among clinical isolates of ST1 (BI/NAP1/027)Clostridioides difficile

Abstract: Clostridioides difficile (C. difficile), a leading cause of nosocomial infection, produces toxins that damage the colonic epithelium and results in colitis that varies from mild to fulminant. Variation in disease severity is poorly understood and has been attributed to host factors (age, immune competence and intestinal microbiome composition) and/or virulence differences between C. difficile strains, with some, such as the epidemic BI/NAP1/027 (MLST1) strain, being associated with greater virulence. We tested… Show more

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Cited by 7 publications
(26 citation statements)
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“…Importantly 4a). The latter finding supports previous data indicating that clade 2 isolates are hypervirulent, often attributed to the presence of the binary toxin operon or increased expression from the PaLoc 19,56,57 . Meanwhile, some clade 1 isolates contain no toxin genes, indicating a diversity of colonization strategies in this lineage.…”
Section: Carriers and CDI Patients Contribute To Transmission Network...supporting
confidence: 91%
“…Importantly 4a). The latter finding supports previous data indicating that clade 2 isolates are hypervirulent, often attributed to the presence of the binary toxin operon or increased expression from the PaLoc 19,56,57 . Meanwhile, some clade 1 isolates contain no toxin genes, indicating a diversity of colonization strategies in this lineage.…”
Section: Carriers and CDI Patients Contribute To Transmission Network...supporting
confidence: 91%
“…Protection by the avirulent ST1-75 is not due to differences in the lysogenic phages between strains. ST1-75 harbors two unique prophages in its genome, namely phiCD75-2 and phiCD75-3, which can become infectious upon induction (27). Prophages can determine the susceptibility of the bacterial host to phage infections via superinfection exclusion or other anti-phage defense systems (30)(31)(32).…”
Section: Resultsmentioning
confidence: 99%
“…Thus, analyzing more strains within a clade may reveal fewer differences between clades. A caveat to our analyses is that C. difficile strains exhibit considerable genetic diversity even within a ribotype (20,22), so our conclusions may only reflect the behavior of RT078 Clade 5 strains. It is also possible that analyzing more strains within this ribotype will reveal a broader spectrum of phenotypes than the somewhat bimodal ones we have reported with respect to cell length and chaining propensity (Figures 2, 3).…”
Section: Discussionmentioning
confidence: 95%
“…Notably, RT027 (ST1), RT017 (ST45), and RT078 (ST11) strains are from ribotypes/ multilocus sequencing types that are frequently isolated from patients with C. difficile infection (CDI) (12,19,22,25).…”
Section: Time-lapse Microscopy Reveals Clade-specific Differences In ...mentioning
confidence: 99%
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