Virulence and horizontal transmission of Beauveria pseudobassiana S.A. Rehner &amp; Humber in Ips sexdentatus and Ips typographus (Coleoptera: Curculionidae)

Kocaçevik, Seda; Sevim, Ali; Eroğlu, Mahmut; Demirbağ, Zihni; Demir, İsmail

doi:10.3906/tar-1504-64

Cited by 29 publications

(18 citation statements)

References 34 publications

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“…The results of this study are in line with those of previous studies as many researchers have reported that efficacy of EPF increases according to concentration increase. Therefore, the LT 50 and LT 90 values of the fungi decreased (Kocaçevik et al 2016and Kushiyev et al 2017, 2018. Kushiyev et al (2017) found that LT 50 and LT 90 values of Fig.…”

Section: Resultsmentioning

confidence: 94%

Efficacy of native isolates of Metarhizium anisopliae and Beauveria bassiana against the invasive ambrosia beetle, Xylosandrus germanus Blandford (Coleoptera: Curculionidae: Scolytinae)

Tuncer

Kushiyev

Erper

et al. 2019

Egypt J Biol Pest Control

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The invasive ambrosia beetle, Xylosandrus germanus Blandford (Coleoptera: Curculionidae: Scolytinae), is one of the most significant pests in hazelnut orchards in Turkey. Chemical control of the beetle is very difficult, because it spends the majority of its life in the sapwood of host trees. In this study, entomopathogenic fungi (EPF), Metarhizium anisopliae (TR-106 isolate), and Beauveria bassiana (TR-217 isolate) were tested against the female adults of X. germanus under laboratory conditions by two different methods. In the first method, conidial concentrations (1 × 10 6 and 1 × 10 8 conidia ml −1 ) of the fungal isolates were directly applied to the beetles in Petri dishes (2 ml per dish), using a Potter spray tower. In the second method, the same conidial concentrations were applied on a sterile hazelnut branch placed in the Petri dishes, and then, beetles were released in dishes. The Petri dishes were incubated at 25 ± 1°C and 75 ± 5% RH, 16:8-h light:dark photoperiod. Lethal time values (LT 50 and LT 90 ) for the isolates were calculated, using Probit analysis. As a result, LT 50 and LT 90 values of M. anisopliae isolate at 1 × 10 8 conidia ml −1 were 4.43 and 6.01 days, respectively, at the beetle application method and 3.97 and 5.68 days, respectively, at the branch application method. LT 50 and LT 90 values were 6.03 and 10.80 days, respectively, at 1 × 10 8 conidia ml −1 , when B. bassiana was applied directly on the beetles and 5.96 and 11.79 days at the same concentration, when it was applied on the branch application method. M. anisopliae at 1 × 10 8 conidia ml −1 caused 100% mortality rate at the end of the 8th day in the two application methods. The mortality rates when applying 1 × 10 8 conidia ml −1 of B. bassiana recorded 64% and 80% by the end of the 8th day on branch and insect application methods, respectively. The efficiency of 1 × 10 6 conidia ml −1 of the isolates was lower than 1 × 10 8 conidia ml −1 against X. germanus in both application methods. This study showed that the isolates of TR-106 and TR-217 had a significant potential as biological control agents against X. germanus.

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Section: Resultsmentioning

confidence: 94%

Efficacy of native isolates of Metarhizium anisopliae and Beauveria bassiana against the invasive ambrosia beetle, Xylosandrus germanus Blandford (Coleoptera: Curculionidae: Scolytinae)

Tuncer

Kushiyev

Erper

et al. 2019

Egypt J Biol Pest Control

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“…Then, the dead insects (belonging to S. granarius and S. oryzae) were washed three times in sterile distilled water and placed in 75 ± 5% RH, using the Memmert incubator. Mortality rates were confirmed by examining of hyphae on the cadavers under Leica EZ4 stereomicroscope, 10 days after placing the dead insects (Kocaçevik et al 2016). The bioassay was performed by using a completely randomized experimental design with five replicates.…”

Section: Experimental Designmentioning

confidence: 99%

Efficacy of entomopathogenic fungi against the stored-grain pests, Sitophilus granarius L. and S. oryzae L. (Coleoptera: Curculionidae)

2019

Egypt J Biol Pest Control

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Efficacy of the five native entomopathogenic fungi (EPFs), Beauveria bassiana, Isaria fumosorosea, Lecanicillium muscarium, Metarhizium anisopliae, and Simplicillium lamellicola, against adults of the stored-grain insect pests, Sitophilus granarius and Sitophilus oryzae (Coleoptera: Curculionidae), was evaluated under laboratory conditions at two different temperatures. Conidial suspensions (1 × 10 8 conidia ml −1 ) of the fungi were directly applied to both the pests in Petri dishes (2 ml per dish), using a Potter spray tower. All the dishes were incubated both at 20 and 25°C in 16 h light/8 h dark and in 75 ± 5% relative humidity (RH). Dead individuals were counted daily, following treatments, for 7 days. Lethal time values (LT 50 and LT 90 ) for EPFs were calculated. The results demonstrated that the mortality rates varied according to both the temperature and the tested EPFs. The highest effect among EPFs at (1 × 10 8 conidia ml −1 ) concentration on S. granarius at 20°C at the end of day 7 was showed by I. fumosorosea (92.69%) and M. anisopliae (90.35%), followed by the other EPFs. Similarly, M. anisopliae and I. fumosorosea were the most effective ones with 90.48 and 84.21% mortality rates, respectively, at 25°C. However, while M. anisopliae (85.68%) showed the highest effect among all the EPFs applied on S. oryzae at 20°C, B. bassiana with a mortality rate of 93.66% was the most effective one at 25°C. LT 50 values for I. fumosorosea and M. anisopliae were 2.75 and 2.88/days, respectively, and LT 90 values were 4.17 and 4.47/ days, respectively, at 20°C for S. oryzae. However, LT 50 values for M. anisopliae on S. granarius in both temperatures were the lowest. This study indicated that M. anisopliae and I. fumosorosea had a significant potential as a biological control agent against S. granarius and S. oryzae. Further studies are necessary to evaluate the efficacy of the isolate on the pests under storage conditions.

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“…; Kocacevik et al. , ). Even though Hypocreales fungi invade their hosts directly through the cuticle of insects, fungal inoculation methods remain a major challenge in the case of most bark beetle species because of their cryptic behaviour.…”

Section: Introductionmentioning

confidence: 99%

“…Sorokin and Lecanicillium spp. W. Gams & Zare, was considered to be suitable as biological control agents against bark beetle populations (Kreutz et al 2004;Wegensteiner 2004;Tanyeli et al 2010;Kocacevik et al 2015Kocacevik et al , 2016. Even though Hypocreales fungi invade their hosts directly through the cuticle of insects, fungal inoculation methods remain a major challenge in the case of most bark beetle species because of their cryptic behaviour.…”

Section: Introductionmentioning

confidence: 99%

Susceptibility of Dendroctonus simplex to Hypocreales fungi: towards the development of a biological control strategy

Srei¹,

Lavallée

Guertin³

2016

J Applied Entomology

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The use of Hypocreales fungi against bark beetles is an attractive alternative strategy for biological control in forestry. However, fungal inoculation methods remain a major challenge due to the cryptic behaviour of these beetles. Experiments were conducted to evaluate the susceptibility of adult eastern larch beetles, Dendroctonus simplex, to different isolates of Hypocreales fungi and to assess the efficiency of direct inoculation of fungal conidia using an assisted autodissemination device. Among the nine fungal isolates tested, Beauveria bassiana INRS‐242 was selected as the utmost isolate causing a mortality rate of 64.4% of D. simplex adults within 10 days and having a high conidial viability of 81.7%. Using an assisted autodissemination device, a fungal‐coated pouch was a promising contamination chamber. Once it had fallen onto the fungal‐coated pouch, each D. simplex adult loaded an average lethal dose of 2.1 × 109 conidia, which induced a mortality rate of 98.0%. Our results demonstrated the fungal pathogenicity of B. bassiana INRS‐242 against the eastern larch beetles and pointed out the potential of the assisted autodissemination device as an effective method of conidia inoculation. These findings represent the first step to develop a biological control strategy against D. simplex populations.

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Virulence and horizontal transmission of Beauveria pseudobassiana S.A. Rehner & Humber in Ips sexdentatus and Ips typographus (Coleoptera: Curculionidae)

Cited by 29 publications

References 34 publications

Efficacy of native isolates of Metarhizium anisopliae and Beauveria bassiana against the invasive ambrosia beetle, Xylosandrus germanus Blandford (Coleoptera: Curculionidae: Scolytinae)

Efficacy of native isolates of Metarhizium anisopliae and Beauveria bassiana against the invasive ambrosia beetle, Xylosandrus germanus Blandford (Coleoptera: Curculionidae: Scolytinae)

Efficacy of entomopathogenic fungi against the stored-grain pests, Sitophilus granarius L. and S. oryzae L. (Coleoptera: Curculionidae)

Susceptibility of Dendroctonus simplex to Hypocreales fungi: towards the development of a biological control strategy

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