Virulence attitude estimation of Pasteurella multocida isolates in embryonated chicken eggs

Shalaby, Azhar G.; Bakry, Neveen R; El-Demerdash, Azza S.

doi:10.1007/s00203-021-02579-x

Cited by 11 publications

(14 citation statements)

References 37 publications

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“…The majority were of capsular type A (96.6%; 29/30). This is consistent with other studies (Mohamed and Mageed 2014;Abd-Elsadek et al 2021;Shalaby et al 2021) which isolated P. multocida in nearly similar percentages and found capsular type A to be the most common capsular type among avian strains. Interestingly, capsular type D was detected in 3.4% (1/30) of isolated P. multocida from quails, a serogroup considered rare (Glisson et al 2008), demonstrating a variable prevalence of the serogroups in cases of respiratory disease according to the geographic region (Davies et al 2003).…”

Section: Discussionsupporting

confidence: 93%

“…Due to its Studies of pathognomonic features of P. multocida isolates among various avian species have shown significant genetic variations among P. multocida strains from each avian species (Hurtado et al 2020). These variations were attributed to many factors, including difference in host immune systems, various environmental conditions, and different transmission routes which have implications for the pathogenicity, diagnosis, treatment, and prevention of P. multocida infections in avian species (Saha et al 2021;Shalaby et al 2021).…”

Section: Discussionmentioning

confidence: 99%

“…These assays were conducted in the Biotechnology Unit, Animal Health Research Institute, Zagazig Branch, Egypt, following a manual previously published in Shalaby et al ( 2021 ).…”

Section: Methodsmentioning

confidence: 99%

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Pathognomonic features of Pasteurella multocida isolates among various avian species in Sharkia Governorate, Egypt

El-Demerdash,

Mowafy,

Fahmy

et al. 2023

World J Microbiol Biotechnol

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The present study aimed to isolate Pasteurella multocida (P. multocida) from pulmonary cases in several avian species and then investigate the histopathological features, antimicrobial resistance determinants, virulence characteristics, and risk factors analysis of the isolates in each species in correlation with epidemiological mapping of pasteurellosis in Sharkia Governorate, Egypt. The obtained data revealed a total occurrence of 9.4% (30/317) of P. multocida among the examined birds (chickens, ducks, quails, and turkeys). The incidence rate was influenced by avian species, climate, breed, age, clinical signs, and sample type. Antimicrobial susceptibility testing revealed that all isolates were sensitive to florfenicol and enrofloxacin, while 86.6 and 73.3% of the isolates displayed resistance to amoxicillin-clavulanic acid and erythromycin, respectively. All of the P. multocida isolates showed a multiple-drug resistant pattern with an average index of 0.43. Molecular characterization revealed that the oma87, sodA, and ptfA virulence genes were detected in the all examined P. multocida isolates. The ermX (erythromycin), blaROB-1 (β-lactam), and mcr-1(colistin) resistance genes were present in 60, 46.6, and 40% of the isolates, respectively. Ducks and quails were the most virulent and harbored species of antimicrobial-resistant genes. These results were in parallel with postmortem and histopathological examinations which detected more severe interstitial pneumonia lesions in the trachea and lung, congestion, and cellular infiltration especially in ducks. Epidemiological mapping revealed that the Fakous district was the most susceptible to pasteurellosis infection. Thus, farmers are recommended to monitor their flocks for signs of respiratory disease, seek veterinary care promptly if any birds are sick, and avoid the random usage of antibiotics. In conclusion, this study presents a comprehensive picture of the risk factors in correlation to the pathognomonic characteristics of P. multocida infection in poultry sectors to help in developing more effective strategies for prevention and control.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

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Pathognomonic features of Pasteurella multocida isolates among various avian species in Sharkia Governorate, Egypt

El-Demerdash,

Mowafy,

Fahmy

et al. 2023

World J Microbiol Biotechnol

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“…Antibiotic resistance can develop as a result of excessive antibiotic usage [ 3 , 42 , 43 ]. In this study, the antimicrobial resistance profile among Salmonella isolates was nearly similar to those recorded in Egypt [ 6 ], China [ 44 ], and Bangladesh [ 31 ].…”

Section: Discussionmentioning

confidence: 99%

An Alternative Approach Using Nano-garlic Emulsion and its Synergy with Antibiotics for Controlling Biofilm-Producing Multidrug-Resistant Salmonella in Chicken

El-Demerdash,

Orady,

Matter

et al. 2023

Indian J Microbiol

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Surface-growing antibiotic-resistant pathogenic Salmonella is emerging as a global health challenge due to its high economic loss in the poultry industry. Their pathogenesis, increasing antimicrobial resistance, and biofilm formation make them challenging to treat with traditional therapy. The identification of antimicrobial herbal ingredients may provide valuable solutions to solve this problem. Therefore, our aim is to evaluate the potency of nano garlic as the alternative of choice against multidrug-resistant (MDR) Salmonella isolates using disc diffusion and microdilution assays. Then, checkerboard titration in trays was applied, and FIC was measured to identify the type of interaction between the two antimicrobials. A disc diffusion assay revealed that neomycin was the drug of choice. The range of nano garlic MIC was 12.5–25 μg/ml, while the neomycin MIC range was 32–64 μg/ml. The FIC index established a synergistic association between the two tested drugs in 85% of isolates. An experimental model was used including nano garlic and neomycin alone and in combination against Salmonella infection. The combination therapy significantly improved body productivity and inhibited biofilm formation by more than 50% down regulating the CsgBAD, motB, and sipA operons, which are responsible for curli fimbriae production and biofilm formation in Salmonella serotypes.

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“…Due to its simplicity, speed, and specificity, polymerase chain reaction (PCR) applications have been widely used ( Kesmen et al ., 2007 ; El-Demerdash et al ., 2023a ). According to several studies ( Abuelnaga et al ., 2020 ; Shalaby et al ., 2021 ; Megahed et al ., 2023 ), multiplex PCR is a powerful approach that can simultaneously amplify several templates, lower the detection rate, and get beyond the restrictions of a single PCR that only targets one type of meat at a time.…”

Section: Introductionmentioning

confidence: 99%

Bacteriological evaluation and advanced SYBR-green multiplex real-time PCR assay for detection of minced meat adulteration

Sheikh,

Whab,

ElDaly

et al. 2024

Open Vet J

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Background: Minced meat is a valuable source of nutrients, but it is vulnerable to contamination by microorganisms commonly present in the environment. Additionally, there is a risk of adulteration with cheaper meat sources, which can be harmful to consumers. Aim: It is crucial to identify meat adulteration with distinct microbiological analysis for legal, economic, religious, and public health purposes. Methods: A total of 100 minced meat samples were collected from several markets in Sharkia Governorate, Egypt. These samples were then subjected to bacteriological testing and an advanced multiplex PCR method. This method enables the detection of bovine, equine, porcine, and dog species in meat samples with just one step. Results: The adulterated samples had a higher total bacterial count and pH values compared to pure bovine meat. These differences in bacterial count and pH values were statistically significant, with p-values of 0.843 (log10) and 0.233, respectively. The frequency of E. coli occurrence was 13%, and the O111 serotype was predominant in the adulterated samples. Listeria monocytogenes and S. aureus were isolated with prevalence rates of 3% and 29%, respectively. Besides, the SYBR-green multiplex real-time PCR assay used in this study detected adulteration with dog, equine, and porcine meats in the examined samples at rates of 9%, 5%, and 4%, respectively. Conclusion: This method provides a sensitive and specific approach to detect issues related to well-being and safety.

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Virulence attitude estimation of Pasteurella multocida isolates in embryonated chicken eggs

Cited by 11 publications

References 37 publications

Pathognomonic features of Pasteurella multocida isolates among various avian species in Sharkia Governorate, Egypt

Pathognomonic features of Pasteurella multocida isolates among various avian species in Sharkia Governorate, Egypt

An Alternative Approach Using Nano-garlic Emulsion and its Synergy with Antibiotics for Controlling Biofilm-Producing Multidrug-Resistant Salmonella in Chicken

Bacteriological evaluation and advanced SYBR-green multiplex real-time PCR assay for detection of minced meat adulteration

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