Virulence Factor Profiles of Escherichia coli Isolated from Pork and Chicken Meats Obtained from Retail Markets

Abstract

The aim of this study was to investigate the virulence factor profiles of Escherichia coli strains isolated from pork and chicken meats purchased from retail markets in Korea. From 943 pork and 142 chicken meats, 217 isolates of E. coli were cultured. The presence of 11 virulence factors (elt, estI, estII, astA, stx, cdt, cnf, agg, inve, eae, afa) were detected by polymerase chain reaction. Forty one (18.9%) of 217 E. coli isolates carried at least one virulence factor. Among 175 E. coli isolates from pork, the detection rate of astA, elt, eae, estII, estI, afa, and cnf were 6.9%, 4.6%, 4.6%, 4.0%, 2.3%, 1.1%, and 0.6%, respectively. However, stx, agg, and cdt were not detected in our isolates. Therefore, we conclude that astA is the most prevalent virulence factor in E. coli isolates contaminated in pork and chicken meats in Korea.

Virulence Factor Profiles of Escherichia coli O157:H7 Bacteriophage Isolates from Sewage and Livestock Stools

“…공시균주 및 박테리오파지 분리 가검물 현재까지 알려진 대장균의 병원성인자 stx1, stx2, eae, aafII, ial, elt, estI, estII, astA, afa, cnf 를 검출하기 위한 양성대조 균주는 선행연구에서 사용한 대장균 표준균주와 분리주를 사용하였다 [10][11] . [12][13][14] .…”

“…coli 병원성인자 stx1, stx2, eae, aafII, ial, elt, estI, estII, astA, afa, cnf에 대한 중합효소연쇄반응은 선행연구 에서 사용된 방법에 따라서 수행되었다 [10][11] . 중합효소연쇄 반응을 위한 primer는 모두 Bioneer에 의뢰하여 제작되었 다( Table 1).…”

A Multiplex PCR Assay for the Detection of Food-borne Pathogens in Meat Products

“…The pathogen has been implicated in large food-borne outbreaks all over the world, including in Canada, UK, Scotland, and Japan. Escherichia coli O157:H7 has been shown to cause several disease syndromes, including diarrhea, hemorrhagic colitis, hemolytic uremic syndrome, and thrombotic thrombocytopenic purpura in humans (Choi et al, 2010;Gooding and Choudary, 1997). Additionally, enterotoxigenic Staphylococcus aureus is one of the major pathogens responsible for cases of food poisoning all over the world (Dinges et al, 2000).…”