Virulence factors and clinical patterns of multiple-clone hypermucoviscous KPC-2 producing K. pneumoniae

Abstract

Carbapenemase-producing Klebsiella pneumoniae (CRKP) are increasingly reported worldwide being necessary the local epidemiological monitoring. Our aim was to characterize the hypermucoviscous CRKP isolates collected in our hospital during a 6 months period. Carriage of the carbapenemase genes ( bla KPC , bla NDM , bla VIM and bla OXA-48 ), extended spectrum β-lactamases ( bla SHV-2 , bla CTX-M ) and the virulence genes ( mag A, k2A, rmp A, wab G, uge , all S, ent B, ycf M, kpn , wca G, fim H, mrk D, iut A, iro N, hly and cnf -1) were determined by multiplex-PCR. Genetic relationship among the isolates was performed by PFGE and MLST. A total of 35 isolates were recovered, being the urinary and respiratory tract the most common infection sites (34.2%). The bla KPC-2 gene was present in all the isolates, coexisting with bla CTX-M-2 (45.7%), bla SHV-2 (28.6%), and bla CTX-M-2 / bla SHV-2 (14.3%). The capsular serotype K2 corresponded with 68.6% of the isolates. Virulence factors frequency were variable [adhesins (97.1%), siderophores (94.3%) and phagocytosis resistance ( wab G 48.5%, uge 80% and ycf M 57.1%)]. A total of 10 STs were identified although 40% of them clustered on ST25-CC65, and 17% to ST17. The incidence of KPC-2-producing K. pneumoniae reported by the hospital was 0.290 per 1000 admissions. In summary we described an epidemic scenario of multidrug resistant hypermucoviscous KPC-2 producing ST25 K. pneumoniae in our institution.

Effective Photodynamic Therapy with Ir(III) for Virulent Clinical Isolates of Extended-Spectrum Beta-lactamase <em>Klebsiella pneumoniae</em>

“…The ESBL-producing strains of K. pneumoniae of the genotype K2 + , ybtS + , and allS + were more resistant to innate immunity, consistently with studies over MDR populations of K. pneumoniae that increased their 30-day mortality over patients undergoing bloodstream infections [1,48]. Also, virulence genes, such as siderophores, which have an essential role in bacterial survival and virulence [49,50], have been previously associated with the MDR-K. pneumoniae [16,51]. In this study, ESBL-producer K. pneumoniae of the K2 + , ybtS +, and allS + genotype shown a survival improvement for killing by PMNs.…”

Antibacterial, antibiofilm, and antiquorum sensing activities of phytosynthesized silver nanoparticles fabricated fromMespilus germanicaextract against multidrug resistance ofKlebsiella pneumoniaeclinical strains

“…Different virulence factors, including fimbriae, antiphagocytic capsule, lipopolysaccharides, membrane transporters, and siderophores, enable the viability of K. pneumoniae and its immune evasion against host defense [5]. Biofilm-forming K. pneumoniae strains protect themselves from host immune responses as well as from antibiotics, increasing their pathogenesis [6,7].…”

Molecular Epidemiology of and Risk Factors for Extensively Drug-Resistant Klebsiella pneumoniae Infections in Southwestern China: A Retrospective Study

“…The data revealed that the emergence of carbapenemase genes and ESBLs was the primary resistance mechanism. Among them, KPC-2 and CTX-M-9 group genes were the most prevalent in this study and were reported to be the most easily transmitted horizontally in KP (Fuga et al, 2019; Perez, 2019; Vargas et al, 2019). In addition, DHA was reported to be the most common gene in KP producing AmpC β-lactamases in many studies, which was consistent with our study (Akata et al, 2019; Kazemian et al, 2019).…”