Virulence factors of Burkholderia cepacia

Nelson, J

doi:10.1016/0928-8244(94)90033-7

FEMS Immunology and Medical Microbiology

1994

DOI: 10.1016/0928-8244(94)90033-7

|View full text |Cite

Virulence factors of Burkholderia cepacia

J Nelson¹

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

1994

2002

Publication Types

Select...

Article10

Relationship

Self Cite0

Independent10

Authors

Journals

Cited by 32 publications

(38 citation statements)

References 50 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It also holds promise as an agent of biocontrol of many plant pathogens (7,8,20) and as a bioremediation organism for the degradation of a wide range of recalcitrant compounds (21,23). Although several attempts have been made to distinguish between benign and human pathogenic strains of Bcc, clear demarcations between environmentally useful and clinically significant strains have not been found (11,28,34,47,49,50). Moreover, a small but steady number of Bcc infections in cystic fibrosis patients each year occur from strains that have not been previously encountered in the clinical setting (26).…”

mentioning

confidence: 99%

Culture-Based and Non-Growth-Dependent Detection of the Burkholderia cepacia Complex in Soil Environments

Miller¹,

LiPuma

Parke

2002

Appl Environ Microbiol

View full text Add to dashboard Cite

Burkholderia cepacia complex (Bcc) bacteria reside in soil, plant rhizospheres, and water, but their prevalence and distribution in outdoor environments is not clear. We sampled a variety of soil and rhizosphere environments with which people may have contact: playgrounds, athletic fields, parks, hiking trails, residential yards, and gardens. A total of 91 sites was sampled in three large U.S. cities. In the first phase of the study, putative Bcc isolates were recovered on Burkholderia cepacia selective agar and trypan blue tetracycline medium and subsequently examined for biochemical reactivity and growth at 32 and 22°C. Isolates were further examined by PCR assays targeting Bcc-specific ribosomal DNA and recA gene sequences. Among the 1,013 bacterial isolates examined, 68 were identified as Bcc; 14 (15%) of 91 sampled sites yielded Bcc isolates. In the second phase, DNA was extracted directly from soil samples and examined with PCR assays targeting Bcc 16S rRNA gene sequences. Either 82 or 93% of the soil samples were positive for at least one Bcc genomovar, depending on the PCR assay system used. Cloning and sequencing were performed to check the specificity of the PCR assays. Sequence analysis of the 463-bp 16S rRNA inserts from eight clones indicated that all were from members of the Bcc. The four soil samples from which these clones were generated did not yield isolates identified as Bcc. Based on PCR detection, Bcc appears to be prevalent in soil from urban and suburban environments. Culture-based recovery of Bcc may underestimate environmental populations.

show abstract

mentioning

confidence: 99%

Culture-Based and Non-Growth-Dependent Detection of the Burkholderia cepacia Complex in Soil Environments

Miller¹,

LiPuma

Parke

2002

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

“…Why CF predisposes patients to acquisition of B. cepacia is incompletely understood. Host lung factors such as underlying lung damage (16,23), repeated exposure (15,28,37,53), and specific bacterial factors, such as presence of cable pili (46)(47)(48), production of extracellular enzymes (30,39), and the ability of some strains of B. cepacia to replicate intracellularly (3,33,43), all appear to contribute to the propensity to persistent infection. One feature that has thwarted the identification of virulence properties is that B. cepacia is not a single clonal strain.…”

mentioning

confidence: 99%

Enhanced Susceptibility to Pulmonary Infection withBurkholderia cepaciainCftr^−/−Mice

et al. 2001

View full text Add to dashboard Cite

Progressive pulmonary infection is the dominant clinical feature of cystic fibrosis (CF), but the molecular basis for this susceptibility remains incompletely understood. To study this problem, we developed a model of chronic pneumonia by repeated instillation of a clinical isolate of Burkholderia cepacia (genomovar III, ET12 strain), an opportunistic gram-negative bacterium, from a case of CF into the lungs of Cftr m1unc؊/؊ (Cftr ؊/؊ ) and congenic Cftr ؉/؉ controls. Nine days after the last instillation, the CF transmembrane regulator knockout mice showed persistence of viable bacteria with chronic severe bronchopneumonia while wild-type mice remained healthy. The histopathological changes in the lungs of the susceptible Cftr ؊/؊ mice were characterized by infiltration of a mixed inflammatory-cell population into the peribronchiolar and perivascular spaces, Clara cell hyperplasia, mucus hypersecretion in airways, and exudation into alveolar airspaces by a mixed population of macrophages and neutrophils. An increased proportion of neutrophils was observed in bronchoalveolar lavage fluid from the Cftr ؊/؊ mice, which, despite an increased bacterial load, demonstrated minimal evidence of activation. Alveolar macrophages from Cftr ؊/؊ mice also demonstrated suboptimal activation. These observations suggest that the pulmonary host defenses are compromised in lungs from animals with CF, as manifested by increased susceptibility to bacterial infection and lung injury. This murine model of chronic pneumonia thus reflects, in part, the situation in human patients and may help elucidate the mechanisms leading to defective host defense in CF.

show abstract

“…Instead, over 80% of the patients were cocolonized with P. aeruginosa. Whereas P. aeruginosa produces a panoply of virulence factors which play an active role in the organism's pathogenicity (7,26), little is known about the pathophysiology of B. cepacia (5,14). Isolated strains of B. cepacia are variable in their abilities to produce hemolysins, lipase and protease (5), exopolysaccharide (2,17), and ironchelating siderophores (12,21) in vitro.…”

mentioning

confidence: 99%

Influence of Pseudomonas aeruginosa exoproducts on virulence factor production in Burkholderia cepacia: evidence of interspecies communication

1995

View full text Add to dashboard Cite

The effect of concentrated cell-free extracellular material from stationary-phase cultures of Burkholderia cepacia 10661 and Pseudomonas aeruginosa PAO1 on virulence factor production in B. cepacia was assessed. While increasing concentrations of the B. cepacia exoproduct caused a slight increase in siderophore, lipase, and protease production in the producing organism, a significant increase in productivity was observed for all three virulence factors with the addition of the PAO1 exoproduct. Moreover, the addition of the exoproduct from a strain of P. aeruginosa producing reduced amounts of autoinducer caused only a slightly greater response than that of the control. Both B. cepacia 10661 and P. aeruginosa PAO1, along with two matched clinical isolates of both organisms obtained from a cystic fibrotic patient, were shown to produce variable amounts of three different types of autoinducer. The potential for interspecies signalling in microbial pathogenicity is discussed.Burkholderia cepacia, formerly known as Pseudomonas cepacia, is now recognized as an important opportunistic agent of human disease (6,8). In particular, it has received a great deal of attention owing to its increasing association with fatal pulmonary infections in patients with cystic fibrotic (CF) lung disease (9, 13). Clinically, B. cepacia colonization of CF patients can result in an asymptomatic carriage, a slow and continuous decline in lung function, or third, a rapid deterioration of the lung accompanied by fever, necrotizing pneumonia, and in some cases, bacteremia (6, 9). This third syndrome is not observed with other CF pathogens. Generally, it has been noted that B. cepacia colonizes the lung after infection by other microorganisms (24,25). Indeed, in a recent survey of the population with CF lung disease in Greater Manchester, England, only 3.3% of patients were colonized exclusively with B. cepacia (11). Instead, over 80% of the patients were cocolonized with P. aeruginosa. Whereas P. aeruginosa produces a panoply of virulence factors which play an active role in the organism's pathogenicity (7,26), little is known about the pathophysiology of B. cepacia (5,14). Isolated strains of B. cepacia are variable in their abilities to produce hemolysins, lipase and protease (5), exopolysaccharide (2, 17), and ironchelating siderophores (12, 21) in vitro. Consequently, there has been no direct correlation with any of these virulence factors to the organism's pathogenic status in vivo. However, a novel possibility might be the active coaggregation of B. cepacia and P. aeruginosa in the lungs, whereby one species synergistically enhances the virulence determinants of the other. To this effect, the enhancement of B. cepacia attachment to different surfaces by P. aeruginosa exoproducts has been demonstrated (1, 18).It is now recognized that individual cells within a multicellular system can both generate signals and respond to those produced by the surrounding cells, providing a basis for cells to change in response to prevailing environmental...

show abstract

scite is a Brooklyn-based organization that helps researchers better discover and understand research articles through Smart Citations–citations that display the context of the citation and describe whether the article provides supporting or contrasting evidence. scite is used by students and researchers from around the world and is funded in part by the National Science Foundation and the National Institute on Drug Abuse of the National Institutes of Health.

Contact Info

customersupport@researchsolutions.com

10624 S. Eastern Ave., Ste. A-614

Henderson, NV 89052, USA

This site is protected by reCAPTCHA and the Google Privacy Policy and Terms of Service apply.

Blog Terms and Conditions API Terms Privacy Policy Contact Cookie Preferences Do Not Sell or Share My Personal Information

Made with 💙 for researchers

Part of the Research Solutions Family.

Virulence factors of Burkholderia cepacia

Cited by 32 publications

References 50 publications

Culture-Based and Non-Growth-Dependent Detection of the Burkholderia cepacia Complex in Soil Environments

Culture-Based and Non-Growth-Dependent Detection of the Burkholderia cepacia Complex in Soil Environments

Enhanced Susceptibility to Pulmonary Infection withBurkholderia cepaciainCftr^−/−Mice

Influence of Pseudomonas aeruginosa exoproducts on virulence factor production in Burkholderia cepacia: evidence of interspecies communication

Contact Info

Product

Resources

About

Virulence factors of Burkholderia cepacia

Cited by 32 publications

References 50 publications

Culture-Based and Non-Growth-Dependent Detection of the Burkholderia cepacia Complex in Soil Environments

Culture-Based and Non-Growth-Dependent Detection of the Burkholderia cepacia Complex in Soil Environments

Enhanced Susceptibility to Pulmonary Infection withBurkholderia cepaciainCftr−/−Mice

Influence of Pseudomonas aeruginosa exoproducts on virulence factor production in Burkholderia cepacia: evidence of interspecies communication

Contact Info

Product

Resources

About

Enhanced Susceptibility to Pulmonary Infection withBurkholderia cepaciainCftr^−/−Mice