Virulence Genotyping ofPasteurella multocidaIsolated from Multiple Hosts from India

Abstract

In this study, 108 P. multocida isolates recovered from various host animals such as cattle, buffalo, swine, poultry (chicken, duck, and emu) and rabbits were screened for carriage of 8 virulence associated genes. The results revealed some unique information on the prevalence of virulence associated genes among Indian isolates. With the exception of toxA gene, all other virulence associated genes were found to be regularly distributed among host species. Association study between capsule type and virulence genes suggested that pfhA, nanB, and nanH genes were regularly distributed among all serotypes with the exception of CapD, whereas toxA gene was found to be positively associated with CapD and CapA. The frequency of hgbA and nanH genes among swine isolates of Indian origin was found to be less in comparison to its equivalents around the globe. Interestingly, very high prevalence of tbpA gene was observed among poultry, swine, and rabbit isolates. Likewise, very high prevalence of pfhA gene (95.3%) was observed among Indian isolates, irrespective of host species origin.

Investigation of Iron uptake and virulence gene factors (fur, tonB, exbD, exbB, hgbA, hgbB1, hgbB2 and tbpA) among isolates of Pasteurella multocida from Iran

“…Epidemiological investigations on frequency of bacterial pathogens can help discover the origin of related diseases, which can further provide essential information for applying more efficient control measures (2).…”

“…Pasteurella multocida (P. multocida) is a Gram-negative bacterium that belongs to the Pasteurellaceae family (1,2). It is a nonmotile, facultive anaerobic microbe which can be isolated from a variety of species, including domesticated mammals and poultry throughout the world (3,4).…”

“…It is a nonmotile, facultive anaerobic microbe which can be isolated from a variety of species, including domesticated mammals and poultry throughout the world (3,4). P. multocida causes atrophic rhinitis in swine, snuffle in rabbits, fowl cholera among avian, haemorrhagic septicaemia in bovine, and enzootic bronchopneumonia in both bovine and ovine herds (2,5). There are 5 capsular serogroups: A, B, D, E and F; and according to lipopolysaccharide antigen expression, they are arranged into 16 somatic serotypes (6).…”

“…HgbA gene is reported to be distributed more regularly among isolates, whereas hgbB gene prevalence varies among strains according to the host origin and the animal disease status. Another protein known as TbpA, a transferrin binding protein, is reported as an epidemiological marker among cattle and plays an essential role in extraction of iron from transferrin (2).…”

Prevalence of Pasteurella multocida in Buffaloes (Bubalus bubalis) in Marshes of South of Iraq

“…P asteurella, a Gram-negative coccobacillus, causes many diseases in humans and a wide range of animal species, including poultry, ruminant, cats, pigs and dogs (Al-Ani et al, 1997;Sarangi et al, 2014;Narsana and Farhat, 2015;Shirzad-Aski and Tabatabaei, 2016). Pasteurella multocida infects the lungs, nasopharynx and tonsils and may be disseminated to other organs (Al-Haddawi et al, 2000;Lainson et al, 2002;Davies et al, 2003).…”