VIRULENCE IN NEWCASTLE DISEASE VIRUS: A Preliminary Study

Waterson, A. P.; Pennington, T. H.; Allan, W. H.

doi:10.1093/oxfordjournals.bmb.a070534

Cited by 77 publications

(42 citation statements)

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“…Procedures for categorization and diagnosis of NDV isolates have generally been based on complex and protracted in vivo tests (Waterson et al, 1967). Variations in proteolytic activation of the membrane glycoprotein precursors of different strains offer the prospect of developing simpler, more precise and rapid assay systems for this purpose.…”

Section: A N Hodder and Othersmentioning

confidence: 99%

“…Avian paramyxovirus type 1, commonly known as Newcastle disease virus (NDV), exists as a number of strains which cause disease in poultry to varying degrees of severity (Waterson et al, 1967). This variation in virulence is determined in part by variation in the susceptibilities of the fusion (F) protein biosynthetic precursors (F 0 proteins) of the different strains to proteolytic activation in different cell types (Nagai et al, 1976(Nagai et al, , 1979Klenk et al, 1977).…”

Section: Introductionmentioning

confidence: 99%

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Analysis of pathotype-specific structural features and cleavage activation of Newcastle disease virus membrane glycoproteins using antipeptide antibodies

Hodder

Selleck

White

et al. 1993

Journal of General Virology

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Section: A N Hodder and Othersmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Analysis of pathotype-specific structural features and cleavage activation of Newcastle disease virus membrane glycoproteins using antipeptide antibodies

Hodder

Selleck

White

et al. 1993

Journal of General Virology

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“…NDV has long been considered a good model with which to study variations in viral pathogenicity because of the wide range of virulence of NDV strains (Waterson et al, 1967). When nucleotide sequence information from several strains or mutants of NDV becomes available, it may be possible to correlate features of the sequence with virulence as has been possible for poliovirus and rabies virus (Evans et al, 1985;Dietzschold et al, 1983).…”

Section: Molecular Cloning Of the Viral Genome Provided The Gene Ordementioning

confidence: 99%

Molecular Cloning of Complementary DNA to Newcastle Disease Virus, and Nucleotide Sequence Analysis of the Junction between the Genes Encoding the Haemagglutinin-Neuraminidase and the Large Protein

Chambers

Millar

Bingham

et al. 1986

Journal of General Virology

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SUMMARYComplementary DNA clones to 90 ~o of the Newcastle disease virus (N DV) genome have been produced and mapped. These clones cover the entire HN, F and M genes, most if not all of the L gene and parts of the NP and P genes. The map of overlapping clones gives the gene order 3'-NP-P-M-F-HN-L-5' for NDV, identical to the gene order of Sendai virus, on the assumption that the NP gene of NDV is at the 3' end of the genome as previously suggested by inactivation of NDV transcription by u.v. light. The nucleotide sequence of 453 bases covering the junction between the HN and L genes has been determined. There is nucleotide sequence homology to the consensus polyadenylation and mRNA start sites of Sendai virus and vesicular stomatitis virus. The deduced amino acid sequence of the C terminus of the HN protein of NDV shows homology to the C-terminal amino acid sequences of the HN proteins of simian virus 5 and Sendai virus. An explanation for the presence of HN0, the precursor to HN in some strains of NDV, is suggested by the presence of a long non-coding region at the Y terminus of the mRNA encoding the HN protein of NDV that could, by mutation, allow synthesis of a larger polypeptide.

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“…The severity of the symptoms of the disease varies from asymptomatic infection to near 100% mortality, depending on the virulence of the virus present (Waterson et al, 1967). Proteolytic cleavage of the F 0 precursor glycoprotein to generate the disulphidelinked polypeptides F1 and F2 is necessary for the infectivity of paramyxoviruses (Homma & Ohuchi, 1973 ;Samson & Fox, 1973 ;Scheid & Choppin, 1974;Nagai et al, 1976).…”

Section: Introductionmentioning

confidence: 99%

Nucleotide Sequence of the Gene Encoding the Fusion Glycoprotein of Newcastle Disease Virus

Chambers

Millar

Emmerson

1986

Journal of General Virology

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SUMMARYThe nucleotide sequence of the gene encoding the fusion (F) glycoprotein of the Beaudette C strain of Newcastle disease virus (NDV) has been determined from cDNA clones obtained from virion RNA. The gene is 1792 nucleotides long, including mRNA start and polyadenylation signals typical of paramyxoviruses. The single open reading frame encodes a polypeptide of 553 amino acids, with a predicted molecular weight of 59042. The F polypeptide has three regions of high hydrophobicity: an Nterminal signal peptide, the N terminus of F~ (known from protein sequencing) and a C-terminal membrane-spanning region by which the F glycoprotein is anchored to the membrane. The cleavage site of F0 is located in a highly basic region of the F polypeptide. Five potential asparagine-linked glycosylation sites are present in the amino acid sequence, of which one is in Fz and the others in F~. Comparison of the NDV F amino acid sequence to those from other paramyxoviruses reveals homology to Sendai virus, simian virus 5 and human respiratory syncytial virus. There is also limited homology between the N terminus ofF1 of NDV and the N termini of HA2 of influenza viruses. Post-translational modifications of the NDV F polypeptide are discussed in the light of information provided by the amino acid sequence.

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VIRULENCE IN NEWCASTLE DISEASE VIRUS: A Preliminary Study

Cited by 77 publications

References 0 publications

Analysis of pathotype-specific structural features and cleavage activation of Newcastle disease virus membrane glycoproteins using antipeptide antibodies

Analysis of pathotype-specific structural features and cleavage activation of Newcastle disease virus membrane glycoproteins using antipeptide antibodies

Molecular Cloning of Complementary DNA to Newcastle Disease Virus, and Nucleotide Sequence Analysis of the Junction between the Genes Encoding the Haemagglutinin-Neuraminidase and the Large Protein

Nucleotide Sequence of the Gene Encoding the Fusion Glycoprotein of Newcastle Disease Virus

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