Virulence of Entomopathogenic Nematodes Steinernema glaseri and Heterorhabditis bacteriophora Poinar (HP88 strain) Against the Black Cutworm, Agrotis ipsilon.

Hassan, Heba; Shairra, Souad; Ibrahim, Samah S.

doi:10.21608/eajbsa.2016.12853

Cited by 8 publications

(12 citation statements)

References 35 publications

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“…In addition, Sayed (2011) found a significant decrease in total body tissue homogenate of G. mellonella treated with normal and 2 Gy irradiated S. carpocapsae. Same results obtained by Hassan, Shairra, and Ibrahim (2016) on Agrotis ipsilon treated with S. glaseri and H. bacteriophora. The reason for total protein reduction may attributed to the breakdown of total proteins by proteases; the important extracellular enzymes secreted from the nematode symbiotic bacteria once entering the host body (Bowen, Blackburn, Rocheleau, Grutzmacher, Ffrench, e, & Constant, 2000, 2003Marokhazi et al, 2007).…”

Section: Discussionsupporting

confidence: 77%

Enhancing the efficacy of entomopathogenic nematodes by gamma radiation in controlling Spodoptera littoralis larvae

Sayed

Shairra

2017

Journal of Radiation Research and Applied Sciences

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a b s t r a c tNowadays, finding a safe control program is the aim of all researchers. The goal of this work is to investigate the effect of gamma radiation on the Entomopathogenic nematodes, Steinernema scapterisci and Heterorhabditis bacteriophora Poinar (HP 88 ) efficacy were tested against larvae of cotton leaf worm, Spodoptera littoralis (Boisd.) under laboratory conditions. Results showed that 2 Gy irradiated S. scapterisci and H. bacteriophora were substantially effective in controlling S. littoralis larvae, while H. bacteriophora was more potent in controlling insect larvae. The results revealed that total protein concentration was significantly decreased (P < 0.05) after treatment with normal or irradiated H. bacteriophora or S. scapterisci. In addition, larvae infected with normal S. scapterisci or H. bacteriophora showed a significant elevation in phenoloxidase activity and represented significant reduce after treatment with 2 Gy irradiated S. scapterisci or H. bacteriophora as compared to control group. Also, lysozyme activity was significantly decreased after treatment with irradiated H. bacteriophora, but there was no significance with irradiated S. scapterisci, when compared with control. LDH activity was significantly high (p<0.05) in the haemolymph of larvae treated with normal or irradiated H. bacteriophora or S. scapterisci, as compared to control group. Furthermore among all treatments, 2 Gy irradiated H. bacteriophora was the most potent and efficient in the biomarkers changes. Therefore, it could be concluded that 2 Gy irradiated S. scapterisci and H. bacteriophora can serve within an integrated pest management (IPM) program in an agroecosystem.

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Section: Discussionsupporting

confidence: 77%

Enhancing the efficacy of entomopathogenic nematodes by gamma radiation in controlling Spodoptera littoralis larvae

Sayed

Shairra

2017

Journal of Radiation Research and Applied Sciences

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“…The obtained data show that the damage in DNA parameters were increased proportionally with irradiation doses. These data agreed with those obtained by Galea et al (2016); Hassan et al (2016) and Enciso et al (2018) who studied the effect of gamma irradiation on Heterorhabditis and Steinernema species. They found that, the efficiency was enhanced against G. mellonella.…”

Section: Discussionsupporting

confidence: 91%

“…This efficiency may be attributed to the damage of the antioxidant contents in the insect larval body which could cause the faster larval death (Sayed et al, 2015). The total protein was decreased in the irradiated IJs of S. scapterisci and H. bacteriophora and in turn, decreased in the homogenecity of the G. mellonella body tissue (Sayed, 2011) and of Agrotis ipsilon body tissue (Hassan et al, 2016). These reductions of protein may be due to the breakdown proteases effect on the total protein and the effect of the extracellular enzymes which secreted by the nematode symbiotic bacteria i.e Xenorhabdus for steinernematids and Photorhabdus for herterorhabditids (Poinar, 1979) in the insect body.…”

Section: Discussionmentioning

confidence: 99%

Effect of Gamma Irradiation on Viability, Pathogenicity, Reproductivity of Heterorhabditis bacteriophora and H. indica and detection of DNA Damage

Taha

Shoaib

Abo-Shady

2021

Journal of Plant Protection and Pathology

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Entomopathogenic nematodes (EPNs) are a promising alternative method for insect pest control. The idea of the work is to investigate the effect of gamma irradiation on EPNs pathogenicity under laboratory conditions aiming to enhance their lethal effect against insect pests. Heterorhabditis bacteriophora (Hb) as well as H. indica (Hi) were irradiated directly in nematode suspension and indirectly within the insect cadavers by gamma irradiation dosages of 0, 100, 250, 500, 750 and 1000 Gy. The mortality percentage of nematodes were recorded, the extracted nematodes from the irradiated insect hosts were used to investigate their pathogenicity and reproductivity against Galleria mellonella larvae. The damage of DNA was analyzed using the comet assay and the LC50 of the two nematode species was recorded. The data demonstrated that, the low dosage (100 Gy) enhanced the pathogenicity of EPNs and didn't affect the initial populations (Pi), the final population of emerged IJs (Pf) and the rate of reproduction (Rr%). Moreover, H. bacteriophora was more tolerant to gamma irradiation than H. indica which recorded more DNA damage %, DNA in tail, tail length, tail moment and olive tail moment in the all different irradiated dosages. In conclusion, low dosages of gamma irradiation can be used to enhance the pathogenicity and reproductivity of entomopathogenic nematodes in order to use as a biocontrol agent.

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“…were with the highest activity, giving a 100% mortality to S. littoralis larvae in a Petri dish assay after 24 h post exposure, while S. monticolum caused the highest mortality rate for larvae (100%) at the concentration of 1600 IJs/dish for all larval instars. Despite the results obtained from Hassan et al (2016) who reported that the effect of S. glaseri was greater than the nematode, H. bacteriophora, the obtained results agree with those of Shairra and Nouh (2014) who reported that higher concentrations of nematodes caused an acute effect, while the latent effect was observed in the case of lower ones and Shairra (2007) who found a positive relationship between concentration and larval mortality, mainly due to the concentration of IJs. However, the defense reactions against the nematodes and their associated bacteria may play an important role.…”

Section: The Bioassay Of Heterorhabditis Bacteriophora (Hp88)supporting

confidence: 73%

Efficacy of entomopathogenic nematodes against Spodoptera littoralis (Boisd.) and Agrotis ipsilon (H.) (Lepidoptera: Noctuidae)

Sobhy

Abd-Elbary

Harras

et al. 2020

Egypt J Biol Pest Control

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The study aimed to evaluate the efficacy of the EPNs against the larvae of Egyptian cotton leaf worm Spodoptera littoralis (Boisduval) and the black cutworm Agrotis ipsilon (Hufnagel) (Lepidoptera: Noctuidae) in vitro before in vivo study. The susceptibility of both larval species to the entomopathogenic nematode species, Steinernema monticolum and Heterorhabditis bacteriophora, was evaluated under laboratory conditions. The concentration of 400 IJs/dish for S. monticolum achieved up to 97.77 and 95.55% mortality rates of the 5 larval instars from 2nd to 6th instars of S. littoralis and A. ipsilon, respectively after 72 h. The concentration of 800 IJs/dish recorded larval mortality rates of 41.86 to 100% against 2nd to 6th instars of A. ipsilon larvae, after 72 h. At the lowest concentration (50 IJs/dish), the larvae of S. littoralis were more susceptible to H. bacteriophora than the larvae of A. ipsilon. The data indicated that 200 IJs/dish was the most effective concentration for all larval stages of both insect pests because the mortality percentage was 100%.

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Virulence of Entomopathogenic Nematodes Steinernema glaseri and Heterorhabditis bacteriophora Poinar (HP88 strain) Against the Black Cutworm, Agrotis ipsilon.

Abstract: www.eajbs.eg.net Virulence of Entomopathogenic Nematodes Steinernema glaseri and Heterorhabditis bacteriophora Poinar (HP 88 strain) Against the Black Cutworm, Agrotis ipsilon.

Cited by 8 publications

References 35 publications

Enhancing the efficacy of entomopathogenic nematodes by gamma radiation in controlling Spodoptera littoralis larvae

Enhancing the efficacy of entomopathogenic nematodes by gamma radiation in controlling Spodoptera littoralis larvae

Effect of Gamma Irradiation on Viability, Pathogenicity, Reproductivity of Heterorhabditis bacteriophora and H. indica and detection of DNA Damage

Efficacy of entomopathogenic nematodes against Spodoptera littoralis (Boisd.) and Agrotis ipsilon (H.) (Lepidoptera: Noctuidae)

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