Virulence properties and pathogenicity of multidrug-resistant Vibrio harveyi associated with luminescent vibriosis in Pacific white shrimp, Penaeus vannamei

Ibrahim, Wan Nurhafizah Wan; Lee, Kok Leong; Razzak, Laith Abdul; Musa, Nadirah; Danish-Daniel, Muhd; Zainathan, Sandra Catherine; Musa, Najiah

doi:10.1016/j.jip.2021.107594

Cited by 28 publications

(18 citation statements)

References 47 publications

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“…They are produced not only in their native region in the Pacific ocean [ 1 , 2 ], but also in Southeast Asia [ 3 , 4 ]. The development and growth of the PWS industry can be explained by several factors, including their high demand [ 5 , 6 , 7 ], acceptance of formulated feeds [ 8 , 9 , 10 ], and the feasibility of culturing PWS intensively [ 7 , 11 ]. However, the rapid expansion of this species has also prompted an increased incidence of disease as well as a degradation of the physical environment, which can affect PWS growth and health conditions [ 12 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

Effects of Dietary Nucleotide Supplementation on Performance, Profitability, and Disease Resistance of Litopenaeus vannamei Cultured in Indonesia under Intensive Outdoor Pond Conditions

Novriadi

Roigé

Segarra

2022

Animals

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This study evaluated the effects of dietary nucleotide supplementation in Pacific white shrimp, Litopenaeus vannamei, cultured in Indonesia. A total of 22,500 shrimp receiving diets in which fish meal (FM) had been partially replaced with vegetable protein sources were classified into five study groups (4500 shrimp/group) and received different diets for 110 days: 10FM (control group; 10% FM), 6FM (6% FM—low FM and no nucleotide supplementation), 10FMN (10% FM; 0.1% nucleotides), 8FMN (8% FM; 0.1% nucleotides) and 6FMN (6% FM; 0.1% nucleotides). Growth performance, body composition, total hemocyte count (THC), lysozyme activity, and hepatopancreas histopathology were assessed. Organoleptic evaluation and profitability assessments were also performed. In addition, shrimp resistance to a Vibrio harveyi challenge was studied in shrimps after having received the diets for 30 days. Results showed that reducing FM had a negative impact on growth performance and hepatopancreas morphology. Adding nucleotides resulted in better performance and profitability, a healthier histomorphological appearance of the hepatopancreas, and significantly higher survival rates upon challenge with V. harveyi, while it did not negatively affect organoleptic parameters. In conclusion, nucleotide supplementation could be useful for optimizing performance, profitability, and disease resistance in shrimp cultured under intensive outdoor pond conditions.

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Section: Introductionmentioning

confidence: 99%

Effects of Dietary Nucleotide Supplementation on Performance, Profitability, and Disease Resistance of Litopenaeus vannamei Cultured in Indonesia under Intensive Outdoor Pond Conditions

Novriadi

Roigé

Segarra

2022

Animals

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“…Our study also found that most of the studied bacterial strains (68.7%) were resistant to penicillin. Nurhafizah et al (2021) identified three strains of multidrug-resistant V. harveyi associated with luminescent vibriosis in P. vannamei having MAR index of 0.4. Different species of the genus Vibrio from P. monodon farms of Bangladesh showed multiple antibiotic resistance ( Rahman et al, 2020 ).…”

Section: Discussionmentioning

confidence: 99%

“…MAR index values ≥0.2 indicates that the bacterial strains were previously exposed to heavy antibacterial contamination, and therefore, are at high risk of multi-drug resistance ( Noorlis et al, 2011 ; Nurhafizah et al, 2021 ). The present study found that most of the isolates were resistant to three or more antibiotics having MAR index more than 0.2 including 14 Vibrio spp.…”

Section: Discussionmentioning

confidence: 99%

Molecular identification and antibiotic resistance patterns of diverse bacteria associated with shrimp PL nurseries of Bangladesh: suspectingAcinetobacter venetianusas future threat

Yasin

Begum

Eshik

et al. 2022

PeerJ

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Shrimp aquaculture has been accomplished with breeding and nursing of shrimp in an artificial environment to fulfill the increasing demand of shrimp consumption worldwide. However, the microbial diseases appear as a serious problem in this industry. The study was designed to identify the diverse bacteria from shrimp PL (post-larvae) nurseries and to profile antibiotic resistance patterns. The rearing water (raw seawater, treated and outlet water) and shrimp PL were collected from eight nurseries of south-west Bangladesh. Using selective agar plates, thirty representative isolates were selected for 16S rRNA gene sequencing, antibiotic susceptibility test and MAR index calculation. Representative isolates were identified as Aeromonas caviae, Pseudomonas monteilii, Shewanella algae, Vibrio alginolyticus, V. brasiliensis, V. natriegens, V. parahaemolyticus, V. shilonii, V. xuii, Zobellella denitrificans which are Gram-negative, and Bacillus licheniformis and B. pumilus which are Gram-positive. Notably, six strains identified as Acinetobacter venetianus might be a concern of risk for shrimp industry. The antibiotic resistance pattern reveals that the strain YWO8-97 (identified as P. monteilii) was resistant to all twelve antibiotics. Ceftazidime was the most powerful antibiotic since most of the studied strains were sensitive against it. The six strains of A. venetianus showed multiple antibiotic resistance patterns. MAR index were ranged from 0.08 to 1.0, and values of 26 isolates were more than 0.2 which means prior high exposure to the antibiotics. From the present study, it can be concluded that shrimp PL nurseries in southern part of Bangladesh are getting contaminated with antibiotic resistant pathogenic bacteria.

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“…Furthermore, Vibrio species, such as V. harveyi, V. parahaemolyticus, V. alginolyticus, V. owensii and V. anguillarum were reported as common pathogenic bacteria in various marine animals (Lee et al, 2015;Chen et al, 2017;Deng et al, 2020;Deris et al, 2020;Zhang et al, 2020;Liao et al, 2022;Muthukrishnan et al, 2022). In fact, an overabundance of Vibrio species was found to be intimately related to the progression of shrimp, lobster, and crab diseases such as AHPND, Vibrio-caused bacteraemia, shell disease, luminescent vibriosis and septic hepatopancreatic necrosis disease (Diggles et al, 2000;Vogan et al, 2002;Jayasree et al, 2006;Nie et al, 2017;Morales-Covarrulbias et al, 2018;Xia et al, 2018;Prachumwat et al, 2020;Nurhafizah et al, 2021;Lioa et al, 2022).…”

Section: Discussionmentioning

confidence: 99%

Understanding the effects of salinity and Vibrio harveyi on the gut microbiota profiles of Litopenaeus vannamei

Deris

Iehata

Gan³

et al. 2022

Front. Mar. Sci.

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Increasing evidence have revealed a positive correlation between gut microbiota and shrimp health, in which a healthy shrimp gut consists of a complex and stable microbial community. Given that both abiotic and biotic factors constantly regulate shrimp gut microbiota, any changes affecting the levels of these factors could cause modification to the gut microbiota assemblage. The goal of this study was to explore the effects of salinity levels and pathogenic Vibrio harveyi infection on the diversity, structure, composition, interspecies interaction, and functional pathways of Litopenaeus vannamei gut microbiota. Juvenile shrimp were cultured at 5 ppt, 20 ppt, and 30 ppt for two months prior to Vibrio harveyi infection. After pathogenic V. harveyi challenge test, genomic DNA was isolated from the shrimp gut, and subjected to the 16S rRNA metagenomic sequencing analysis. We observed that gut microbiota diversity of shrimp cultured at 5 ppt and 30 ppt were lower than those cultured at 20 ppt after exposure to V. harveyi infection, suggesting that shrimp cultured at the two former salinity levels were more susceptible to V. harveyi infection. Network analysis also showed that shrimp cultured at 20 ppt exhibit a more stable bacterial network with complex interspecies interaction, even after induced by V. harveyi. Moreover, the presence of a high number of beneficial bacteria such as Pseudoruegeria, Rhodovulum, Ruegeria, Shimia and Lactobacillus in shrimp cultured at 20 ppt might have played a role in inhibiting the growth of V. harveyi and other potentially pathogenic bacteria. Besides, bacterial functional pathway prediction has also shown that metabolic pathways such as phenylalanine metabolism, glycine, serine and threonine metabolism, starch and sucrose metabolism, glyoxylate and dicarboxylate metabolism, carbon metabolism and biofilm formation process were significantly higher in shrimp cultured at 20 ppt. Collectively, our results suggested that 20 ppt is an optimal salinity that suppresses the growth of V. harveyi and potential pathogenic bacteria in the shrimp gut, which could possibly minimize the risk of pathogenic infection for sustainable production of healthy shrimp.

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Virulence properties and pathogenicity of multidrug-resistant Vibrio harveyi associated with luminescent vibriosis in Pacific white shrimp, Penaeus vannamei

Cited by 28 publications

References 47 publications

Effects of Dietary Nucleotide Supplementation on Performance, Profitability, and Disease Resistance of Litopenaeus vannamei Cultured in Indonesia under Intensive Outdoor Pond Conditions

Effects of Dietary Nucleotide Supplementation on Performance, Profitability, and Disease Resistance of Litopenaeus vannamei Cultured in Indonesia under Intensive Outdoor Pond Conditions

Molecular identification and antibiotic resistance patterns of diverse bacteria associated with shrimp PL nurseries of Bangladesh: suspectingAcinetobacter venetianusas future threat

Understanding the effects of salinity and Vibrio harveyi on the gut microbiota profiles of Litopenaeus vannamei

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