Virulence properties of Escherichia coli isolated from Australian dairy powder factory environments

Duffy, Lesley L.; O’Callaghan, David; McAuley, Catherine M.; Fegan, Narelle; Craven, H.M.

doi:10.1016/j.idairyj.2008.09.002

Cited by 7 publications

(9 citation statements)

References 14 publications

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“…The overall contamination rate of E. coli in the samples was 6.0% (32/534), which is much lower than that reported in the Australian dairy powder factory environments (Duffy et al, 2009). The difference in sampling design may account for the discrepancy.…”

Section: Discussionmentioning

confidence: 61%

“…Duffy et al (2009) showed that only 3 (1%) of all environmental E. coli isolates carried the virulence genes escV and eaeA. Several studies (Beutin et al, 1993;Blanco et al, 2003;Rey et al, 2003;Jacob et al, 2013) have shown that goat could be the reservoir of STEC.…”

Section: Discussionmentioning

confidence: 99%

“…All samples were used for the isolation and identification of E. coli (Duffy et al, 2009). Briefly, 10 mL (or 10 g) of samples was mixed with 90 mL of buffered peptone water.…”

Section: Escherichia Coli Enrichment and Isolationmentioning

confidence: 99%

“…It was shown that when considering 60 outbreaks and 4 single cases described in the literature and implicating milk and milk products, pathogenic E. coli were responsible for 11 outbreaks (De Buyser et al, 2001). In 2009, a study revealed the presence of pathogenic E. coli in dairy powder factory environments (Duffy et al, 2009). A study from Ireland showed that the occurrence of the virulence gene of E. coli in raw milk was 36.0%, whereas the occurrence of isolates was 0.8% (Lynch et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

“…And, goat milk-based infant formula is becoming more and more preferred by consumers. However, there is a paucity of data regarding E. coli contamination in goat milk and plants, except a study on the E. coli in dairy powder factory environments (Duffy et al, 2009). The objective of this study was to determine the prevalence of E. coli strains in goat milk powder in the year of 2012 and 2013 from 4 plants in Shaanxi province, China, 2261 and characterize these strains by antimicrobial susceptibility testing and toxin-gene detection.…”

Section: Introductionmentioning

confidence: 99%

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Prevalence, distribution, and diversity of Escherichia coli in plants manufacturing goat milk powder in Shaanxi, China

Xi¹,

Feng²,

et al. 2015

Journal of Dairy Science

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The aim of the study was to investigate the prevalence, distribution, and diversity of Escherichia coli in goat-milk-powder plants in Shaanxi, China. Three plants manufacturing goat milk powder in Shaanxi province were visited once for sampling during 2012 and 2013. Samples were taken for isolation of E. coli. Isolates were characterized by antimicrobial susceptibility testing and detection of virulence genes. All isolates were further examined by pulsed-field gel electrophoresis analysis. In total, 53 E. coli strains were isolated from 32 positive samples out of 534 samples. Among E. coli isolates, resistance was most frequently observed to trimethoprim-sulfamethoxazole (75.5%), whereas all isolates were sensitive to gatifloxacin, kanamycin, amikacin, and amoxicillin-clavulanate. The 6 virulence genes of pathogenic E. coli were not detected. Pulsed-field gel electrophoresis results showed that E. coli strains in plants were genetically diverse and milk storage tank could be an important contamination source. This study could provide useful information for plants manufacturing goat milk powder to establish proper management practices that help minimize the chance of microbial contamination.

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Section: Discussionmentioning

confidence: 61%

Section: Discussionmentioning

confidence: 99%

“…All samples were used for the isolation and identification of E. coli (Duffy et al, 2009). Briefly, 10 mL (or 10 g) of samples was mixed with 90 mL of buffered peptone water.…”

Section: Escherichia Coli Enrichment and Isolationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Prevalence, distribution, and diversity of Escherichia coli in plants manufacturing goat milk powder in Shaanxi, China

Xi¹,

Feng²,

et al. 2015

Journal of Dairy Science

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Cinnamon essential oil and cinnamaldehyde in the control of bacterial biofilms formed on stainless steel surfaces

Oliveira

Brugnera

Nascimento

et al. 2012

Eur Food Res Technol

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The antibacterial eVects of Cinnamomum cassia essential oil (EO) and cinnamaldehyde were evaluated against single-and mixed-species cultivation of enteropathogenic Escherichia coli (EPEC) and Listeria monocytogenes attached to stainless steel. A central composite rotational design with two variables and eleven assays was used to optimize the concentrations (0.00-1.00% v/v for the EO and 0.00-0.80% v/v for cinnamaldehyde) and contact times (1-21 min). The models generated were validated, and the eVectiveness of C. cassia EO and cinnamaldehyde was compared with that of commercially available chemical sanitizers. Cinnamaldehyde and C. cassia EO proved to be eYcient alternatives to commercial chemical sanitizers in the reduction or elimination of sessile bacterial cells. The activity of these natural compounds was, in most cases, equivalent or superior to that of the chemical sanitizers tested. However, L. monocytogenes was more resistant than EPEC to C. cassia EO and cinnamaldehyde, and the bacterial association in mixed-species bioWlms made them more susceptible to these compounds.

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Control of planktonic and sessile bacterial cells by essential oils

Oliveira

Brugnera

Nascimento

et al. 2012

Food and Bioproducts Processing

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Virulence properties of Escherichia coli isolated from Australian dairy powder factory environments

Cited by 7 publications

References 14 publications

Prevalence, distribution, and diversity of Escherichia coli in plants manufacturing goat milk powder in Shaanxi, China

Prevalence, distribution, and diversity of Escherichia coli in plants manufacturing goat milk powder in Shaanxi, China

Cinnamon essential oil and cinnamaldehyde in the control of bacterial biofilms formed on stainless steel surfaces

Control of planktonic and sessile bacterial cells by essential oils

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