Prevalence, distribution, and diversity of Escherichia coli in plants manufacturing goat milk powder in Shaanxi, China

Abstract

The aim of the study was to investigate the prevalence, distribution, and diversity of Escherichia coli in goat-milk-powder plants in Shaanxi, China. Three plants manufacturing goat milk powder in Shaanxi province were visited once for sampling during 2012 and 2013. Samples were taken for isolation of E. coli. Isolates were characterized by antimicrobial susceptibility testing and detection of virulence genes. All isolates were further examined by pulsed-field gel electrophoresis analysis. In total, 53 E. coli strains were isolated from 32 positive samples out of 534 samples. Among E. coli isolates, resistance was most frequently observed to trimethoprim-sulfamethoxazole (75.5%), whereas all isolates were sensitive to gatifloxacin, kanamycin, amikacin, and amoxicillin-clavulanate. The 6 virulence genes of pathogenic E. coli were not detected. Pulsed-field gel electrophoresis results showed that E. coli strains in plants were genetically diverse and milk storage tank could be an important contamination source. This study could provide useful information for plants manufacturing goat milk powder to establish proper management practices that help minimize the chance of microbial contamination.

Isolation and Antimicrobial Susceptibility Profile of Escherichia coli O157 : H7 from Raw Milk of Dairy Cattle in Holeta District, Central Ethiopia

“…e report of Ombarak et al [31] in Egypt also showed that the prevalence of E. coli strains in various types of dairy products had a range of 21 to 77% and both were higher than our findings. e overall prevalence of E. coli strains in raw milk samples of studies conducted on Switzerland [32], Iran [33], Italy [34], Egypt [35], Turkey [36], China [37], and Spain [38] had ranged between 1% and 27% which were comparable with our reported prevalence rate.…”

Prevalence, identification of virulence factors, O-serogroups and antibiotic resistance properties of Shiga-toxin producing Escherichia coli strains isolated from raw milk and traditional dairy products

“…Elhadidy and Mohammed (2012) [ 21 ] revealed that 11.29% of cheese samples were contaminated with the STEC strains which was lower than our findings (34%). Total prevalence of E. coli strains in raw milk and traditional dairy product samples of studies conducted on Switzerland [ 22 ], Iran [ 23 ], Italy [ 24 ], Egypt [ 25 ], Turkey [ 26 ], China [ 27 ], and Spain [ 28 ] had range between 1% to 27% which were lower than our reported prevalence rate.…”

Prevalence and subtyping of Cronobacter species in goat milk powder factories in Shaanxi province, China

“…Goat milk accounts for about 2.3% of the worldwide milk production (Claeys et al, 2014). Goat milk-based infant formula is becoming increasingly preferred by consumers (Xi et al, 2015) because of its specific composition and high nutritional utilization (Alférez et al, 2001;Haenlein, 2004;Mwenze and Kiplagat, 2011). China is the largest goat breeding and production country in the world (Luo, 2009;Thiruvenkadan, 2012), and Shaanxi province is one of the major living areas for goats in China (Luo, 2009).…”