Virulence properties of motile aeromonads isolated from farmed frogs Rana tigerina and R. rugulosa

Pearson, M. D.; Hirono, Ikuo; Aoki, T.; Miranda, Rolando; Inglis, V.

doi:10.3354/dao040185

Cited by 27 publications

(20 citation statements)

References 15 publications

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“…Recently, Pearson et al (2000) reported on the virulence properties of 11 Aeromonas isolates belonging to the Au group. All these isolates displayed high haemolytic activities conferred by ASH1, a haemolysin gene previously cloned from an A. salmonicida strain (Hirono & Aoki, 1993) that was not found in other non-Au frog Aeromonas isolates.…”

Section: Characteristicmentioning

confidence: 99%

“…All these isolates displayed high haemolytic activities conferred by ASH1, a haemolysin gene previously cloned from an A. salmonicida strain (Hirono & Aoki, 1993) that was not found in other non-Au frog Aeromonas isolates. Furthermore, most Au strains produced elastase and were highly cytotoxic to rainbow trout cells but did not significantly affect a mammalian cell line (Pearson et al, 2000). In a supplementary study, clinically healthy Rana rugulosa that were challenged with isolates of the Au group reproduced the symptoms of Aeromonas-associated septicaemia, resulting in mortalities (M. Pearson, unpublished data).…”

Section: Characteristicmentioning

confidence: 99%

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Aeromonas hydrophila subsp. ranae subsp. nov., isolated from septicaemic farmed frogs in Thailand

Huys

Pearson

Kämpfer

et al. 2003

International Journal of Systematic and Evolutionary Microbiology

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A group of seven sucrose-negative Aeromonas strains (referred to as group Au) isolated from the internal organs of septicaemic farmed frogs (Rana rugulosa) in Thailand was subjected to a polyphasic taxonomic study including fluorescent amplified fragment length polymorphism (FAFLP) and ERIC-PCR fingerprinting, 16S rDNA sequencing, microplate DNA-DNA hybridizations and extensive phenotypic characterization. Comparison of FAFLP and ERIC-PCR fingerprints indicated that the group Au isolates belonged to the species Aeromonas hydrophila DNA hybridization group (HG) 1 in which they represent a genotypic subgroup closely affiliated to A. hydrophila subsp. hydrophila and subsp. dhakensis. One representative of the Au group exhibited ¢99?0 % 16S rDNA sequence similarity with the type strains of the two A. hydrophila subspecies. DNA-DNA hybridization with type and reference strains of all known Aeromonas taxa revealed that the Au group represented a homogeneous taxon that exhibited the highest relatedness with members of the two A. hydrophila subspecies, ranging from 75 to 93 %. Phenotypic characterization on the basis of 152 features further revealed that the Au group isolates differed from A. hydrophila subsp. hydrophila or subsp. dhakensis in a total of 13 biochemical properties. Of these, assimilation of L-glycine and isobutyrate as sole carbon source, acid production from salicin and D-sucrose, and aesculin hydrolysis were of diagnostic value. From the results of this study, it can be concluded that the Aeromonas frog isolates of the Au group represent a new subspecies of A. hydrophila, for which the name Aeromonas hydrophila subsp. ranae subsp. nov. is proposed. Its type strain is Au-1D12T (=LMG 19707 T =CCUG 46211 T ).

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Section: Characteristicmentioning

confidence: 99%

Section: Characteristicmentioning

confidence: 99%

Aeromonas hydrophila subsp. ranae subsp. nov., isolated from septicaemic farmed frogs in Thailand

Huys

Pearson

Kämpfer

et al. 2003

International Journal of Systematic and Evolutionary Microbiology

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“…A. hydrophila may infect many species of fresh-water and brackish-water aquacultures from time to time in Asian countries, such as China, Philippines, Thailand, and India [4]. It has long been known as a pathogen of amphibians and reptiles [5,6]. It exhibits many symptoms, such as hemorrhagic septicemia, motile Aeromonas septicemia, infectious abdominal dropsy, bacterial hemorrhagic septicemia, epizootic ulcerative syndrome, hemorrhagic enteritis, and red body disease [1,7,8].…”

Section: Introductionmentioning

confidence: 99%

Immune response and expression analysis of cathepsin K in goldfish during Aeromonas hydrophila infection

Harikrishnan

Kim

et al. 2010

Fish & Shellfish Immunology

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“…Aeromonas hydrophila is a gram-negative enterobacterium widely distributed in aquatic environments (22,24,26,45), and it has long been known as a pathogen of amphibians, reptiles, and fish (4,19,52,(55)(56)(57)66). Also, this bacterial species has been reported to cause a wide variety of human infections (2,28,29,42,70).…”

mentioning

confidence: 99%

Behavior of an Aeromonas hydrophila aroA Live Vaccine in Water Microcosms

Vivas¹,

Carracedo²,

Riaño³

et al. 2004

Appl Environ Microbiol

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Genetically modified auxotrophic mutants of different fish pathogens have been used as live vaccines in laboratory experiments, but the behavior of the strains after release into aquatic ecosystems has not been characterized. We previously constructed and characterized an aroA mutant of Aeromonas hydrophila and studied the protection afforded by this mutant as a live vaccine in rainbow trout. In this work, we describe the survival of this strain in aquatic microcosms prepared from fish water tanks. The aroA mutant disappeared rapidly in nonfiltered, nonautoclaved fish tank water, declining below detection levels after 15 days, suggesting an inhibitory effect of the autochthonous microflora of the water. When the aroA strain was used to inoculate sterilized water, its culturability was lower than that of wild-type strain A. hydrophila AG2; after long periods of incubation, aroA cells were able to enter a viable but nonculturable state. Entry into this nonculturable state was accompanied by changes in the cell morphology from rods to spheres, but the cells appeared to remain potentially viable, as assessed by the preservation of cell membrane integrity. Supplementation of the culture medium with sodium pyruvate favored the culturability and resuscitation of the two A. hydrophila strains at low temperatures (6 and 16°C). These results contribute to a better understanding of the behavior of the aroA strain in natural environments and suggest that the inactivation of the aroA gene may be beneficial for the safety of this live vaccine for aquacultures.

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Virulence properties of motile aeromonads isolated from farmed frogs Rana tigerina and R. rugulosa

Cited by 27 publications

References 15 publications

Aeromonas hydrophila subsp. ranae subsp. nov., isolated from septicaemic farmed frogs in Thailand

Aeromonas hydrophila subsp. ranae subsp. nov., isolated from septicaemic farmed frogs in Thailand

Immune response and expression analysis of cathepsin K in goldfish during Aeromonas hydrophila infection

Behavior of an Aeromonas hydrophila aroA Live Vaccine in Water Microcosms

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