Virus-Based MicroRNA Silencing in Plants

A Role for the F-Box Protein HAWAIIAN SKIRT in Plant microRNA Function

“…The miRNA target mimicry principle has been exploited to investigate the biological function of miRNAs (Franco-Zorrilla et al, 2007;Sha et al, 2014). Artificial miRNA target mimicry lines (MIMs) based on the endogenous IPS1 transcript, or on entirely artificial sequences, have been used to interfere with dozens of Arabidopsis (Arabidopsis thaliana) miRNAs (FrancoZorrilla et al, 2007;Todesco et al, 2010;Yan et al, 2012;Reichel et al, 2015).…”

Recent Advances in In Planta Transient Expression and Silencing Systems for Soybean Using Viral Vectors

“…Viral vectors can be used to investigate miRNA function by expressing miRNA mimics, which would lead to misexpression of the endogenous target mRNAs. Thus, it might be possible to use viral vectors to assess rapidly miRNA function in soybean, as has been shown in N. benthamiana (Sha et al 2014).…”

Sugarcane Mosaic Virus-Based Gene Silencing in Nicotiana benthamiana

“…Gene overexpression and RNA interference (RNAi) studies are presently restricted to the plant species that are acquiescent to the genetic transformation, while, they are laborious and timeconsuming (6,7). Micro-RNA-induced gene silencing (8,9) and artifi cial micro-RNA-mediated gene silencing are used in plants for a targeted gene silencing, but these approaches involve a stable plant transformation, which is laborious, time consuming, and inappropriate with high-throughput studies (10)(11)(12). Similarly, a specifi c gene knockout in the plants can be used by clustered regularly interspaced short palindromic repeats (CRISPR) associated (Cas) nuclease (13), zinc-fi nger nucleases (ZFNs) (14), and transcription activator like eff ector nucleases (TALENs) (15).…”