Virus Elimination Through Meristem Culture and Rapid Clonal Propagation Using a Temporary Immersion System

Shen, Rong-Show; Hsu, Shan–Te

doi:10.1007/978-1-4939-7771-0_14

Cited by 6 publications

(6 citation statements)

References 21 publications

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“…G. skinner showed a higher number of shoots per explant at a lower immersion frequency (2 min each for 4 h). These results are consistent with those reported by Shen and Hsu (2018) for Phalaenopsis in TIS, with 10 min/4 h as the best immersion frequency to achieve optimal development. In contrast, Zhang et al (2018) determined that 3 min/ 6 h was required for the micropropagation of Bletilla striata in TIS.…”

Section: Discussionsupporting

confidence: 92%

“…Micropropagation of orchids has been carried out mainly through conventional culture systems using semisolid medium (Chugh et al 2009;Bhattacharyya et al 2017). However, TIS has been implemented as an alternative that involves lower costs, higher multiplication rates, and seeking to achieve a semi-automated micropropagation process (Murthy et al 2018;Shen et al 2018;Zhang et al 2018). G. skinneri has been micropropagated in semi-solid culture systems only, with higher production costs associated with the use of the gelling agent (Coello et al 2010;Coutiño-Cortés et al 2017).…”

Section: Discussionmentioning

confidence: 99%

“…Various TIS designs have been reported; however, temporary immersion bioreactors (TIB ® ) or twin-jar bioreactors show advantages relative to other systems due to the broad availability of components, different capacity, and easy assembly (Aragón et al 2014;Georgiev et al 2014). Most micropropagation studies of orchids (Orchidaceae) using temporary immersion have involved the use of TIB ® (Murthy et al 2018;Shen and Hsu 2018;Zhang et al 2018). However, according to Murthy et al (2018), the nutritional requirements and particular parameters of TIS micropropagation should be tailored to each species studied.…”

Section: Introductionmentioning

confidence: 99%

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Micropropagation of Guarianthe skinneri (Bateman) Dressler et W. E. Higging in Temporary Immersion Systems

et al. 2020

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This study's aim was to establish a protocol for the micropropagation of G. skinneri using temporary immersion system (TIS). Different concentrations of 6-Benzylaminopurine (0, 1, 2, and 3 mg L −1 ), three different systems of cultivate semi-solid (SS) and liquid media under partial (PI) and temporary immersion systems (TIS), different compositions of the inorganic salts, and the number of subcultures were evaluated. The results showed a maximum of 16.56 shoots per explant obtained through TIS, adjusting all the parameters evaluated in our study. One higher number of shoots per explant was observed in the micropropagation of G. skinneri TIS compared to SS and PI. While the use of 3 mg L −1 of BAP + MS (Murashige and Skoog) media was better than 3 mg L −1 of BAP VW (Vacint and Went) for the generation of a greater number of shoots per explant, 6.33 and 2.72, respectively. The immersion frequency of 2 min every 4 h allowed the production to be scaled to 8.54 shoots per explant. While it was necessary to perform three subcultures every 30 days, to obtain 16.56 shoots per explant, a rooting phase was not required due to the generation of adventitious roots during the different subcultures. However, a phase of elongation of the regenerated plants with ½ MS + GA3 (gibberellic acid) was needed to guarantee 100% survival in the process of acclimatization. In conclusion, this plant production system can be applied for the commercial micropropagation of this species for ornamental purposes, as well as for its reintroduction in protected natural areas.

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Section: Discussionsupporting

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Micropropagation of Guarianthe skinneri (Bateman) Dressler et W. E. Higging in Temporary Immersion Systems

et al. 2020

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“…The technique of culturing apical meristems may also be effective in eliminating viral diseases in orchids, but it requires great manual skill for excision of tiny meristems leading to contamination-free tissue [153]. These requirements and the individual characteristics of viral diseases may lead to breakthroughs in the technique, which may result in in vitro plantlets containing viral diseases, as reported in Brassolaeliocattleya, Cattleya, Dendrobium, Epicattleya, Oncidium, and Mokara grown in vitro, for which CyMV virus was reported to be present in 27.6% of 880 plantlets evaluated, while ORSV was not detected in these samples [152].…”

Section: Applications Of Ipr-plb Technique On Orchid Propagation and mentioning

confidence: 99%

An Overview of Orchid Protocorm-Like Bodies: Mass Propagation, Biotechnology, Molecular Aspects, and Breeding

Cardoso

Zanello

Chen

2020

IJMS

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The process through induction, proliferation and regeneration of protocorm-like bodies (PLBs) is one of the most advantageous methods for mass propagation of orchids which applied to the world floricultural market. In addition, this method has been used as a tool to identify genes of interest associated with the production of PLBs, and also in breeding techniques that use biotechnology to produce new cultivars, such as to obtain transgenic plants. Most of the molecular studies developed have used model plants as species of Phalaenopsis, and interestingly, despite similarities to somatic embryogenesis, some molecular differences do not yet allow to characterize that PLB induction is in fact a type of somatic embryogenesis. Despite the importance of species for conservation and collection purposes, the flower market is supported by hybrid cultivars, usually polyploid, which makes more detailed molecular evaluations difficult. Studies on the effect of plant growth regulators on induction, proliferation, and regeneration of PLBs are the most numerous. However, studies of other factors and new technologies affecting PLB production such as the use of temporary immersion bioreactors and the use of lighting-emitting diodes have emerged as new tools for advancing the technique with increasing PLB production efficiency. In addition, recent studies on Phalaenopsis equestris genome sequencing have enabled more detailed molecular studies and the molecular characterization of plantlets obtained from this technique currently allow the technique to be evaluated in a more comprehensive way regarding its real applications and main limitations aiming at mass propagation, such as somaclonal variation.

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“…At present, orchids are propagated by asymbiotic germination and the tissue culture of vegetative plant material. Both methods allow the production of a large number of disease-free plants and plants that are genetically identical in origin, thus providing useful tools for the ex situ conservation of plants with aesthetic, medicinal, or horticultural value that have become threatened at levels of being critically endangered, endangered, or vulnerable species (Kunakhonnuruk et al 2018, Shen & Hsu 2018.…”

mentioning

confidence: 99%

Asymbiotic Germination, Effect of Plant Growth Regulators, and Chitosan on the Mass Propagation of Stanhopea hernandezii (Orchidaceae)

et al. 2020

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Background: Stanhopea hernandezii was collected from natural habitat in Mexico for its beautiful fragrant flowers. Biotechnological strategies of propagation may satisfy the market demand and are useful for conservation programs. Hypothesis: Vigorous seedlings of S. hernandezii can be produced in vitro by asymbiotic seed germination techniques and the addition of chitosan to the culture medium in the temporary immersion system (RITA®) and in semi-solid medium systems. Methods: The first step was the in vitro germination of seeds obtained from a mature capsule of wild plants, followed by multiplication via adventitious protocorm induction known as protocorm-like bodies, using plant growth regulators. For this purpose, we utilized Murashige and Skoog (MS) basal medium amended with 0.5 mg/L ?-Naphthaleneacetic acid, combined with different concentrations of 6-Benzylaminepurine (1, 3, and 5 mg/L). The following step comprised the growth and development of protocorms to obtain plantlets in RITA® flasks containing 250 mL of liquid MS medium combined or not with different chitosan concentrations (5, 10, 15, 20, and 25 mg/L). Results: The results showed that media supplemented with 5, 10, and 15 mg/L chitosan concentrations enabled the obtaining of a larger biomass with a range of 40-48 seedlings/RITA® and an average height of 13 mm. The last step was the development from seedlings into plantlets, the latter being, vigorous and achieving up to 100 % survival after 12 weeks of ex vitro cultivation. Conclusion: This paper describes an efficient process of asymbiotic germination and mass propagation of S. hernandezii, a vulnerable orchid species endemic to Mexico.

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Virus Elimination Through Meristem Culture and Rapid Clonal Propagation Using a Temporary Immersion System

Cited by 6 publications

References 21 publications

Micropropagation of Guarianthe skinneri (Bateman) Dressler et W. E. Higging in Temporary Immersion Systems

Micropropagation of Guarianthe skinneri (Bateman) Dressler et W. E. Higging in Temporary Immersion Systems

An Overview of Orchid Protocorm-Like Bodies: Mass Propagation, Biotechnology, Molecular Aspects, and Breeding

Asymbiotic Germination, Effect of Plant Growth Regulators, and Chitosan on the Mass Propagation of Stanhopea hernandezii (Orchidaceae)

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