Virus-induced gene silencing for functional analysis of eds1 gene in tomato infected with Ralstonia solanacearum

Yazdi, Hamid Reza Bolok; Sabbagh, Seyed Kazem; Mazaheri, Mahta; Salari, Mohammad; Moshtaghioun, Seyed Mohammad

doi:10.13080/z-a.2018.105.045

Cited by 2 publications

(1 citation statement)

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“…Trilinear decomposition analysis showed the maximum variation of NPR1 and MT-LP genes among three infected genotypes with both MDMV and BgMV viruses. In previous work we showed the important and key role of NPR1 gene in resistance induction in infected tomato plants with Ralstonia solanacearum by using virus induced gene silencing (VIGS) method (Bolok Yazdi et al, 2018). To confirm the critical role of NPR1 and MT-LP genes microarray and VIGS analysis with infected tolerant maize plants with different virus from same family is proposed.…”

Section: Trilinear Decomposition Analysis and Clustering Of Genesmentioning

confidence: 99%

Differential molecular response of maize and Johnson grass against maize dwarf mosaic virus and bermuda grass southern mosaic virus

Mostafavi¹,

Sabbagh²,

Yamchi³

et al. 2019

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Maize dwarf mosaic virus (MDMV) and bermuda grass southern mosaic virus (BgSMV) is the most important cereal potyvirus in Iran. Expression of some key genes in maize plants susceptible and tolerant to MDMV or BgSMV and gene expression profile of MDMV and BgSMV compatible or incompatible to Johnson grass plants were studied. Time points of 1, 9, 24 and 72 h after inoculation with both viruses were investigated as well. By analyzing the expression of the genes, it was identified that in maize infected by MDMV and BgSMV, the transcript levels of the peroxiredoxin, GLP, SAM, NPR1 and chlorophyll a-b binding genes were significantly higher in the tolerant than in susceptible plants during the entire experiment. In the BgSMV inoculated Johnson grass plants, some of the genes related to plant defense responses including NPR1, peroxiredoxin and SAM had higher expression level than the Johnson grass plants inoculated by MDMV. Important genes in maize tolerance like NPR1 and MT-LP, were analyzed by trilinear decomposition analysis and genes clustering. The upregulated expression of genes at one-hour post inoculation showed that the plant response to viruses was activated at the early stage of infection.

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Section: Trilinear Decomposition Analysis and Clustering Of Genesmentioning

confidence: 99%