Virus-Induced Gene Silencing (VIGS) in Flax (Linum usitatissimum L.) Seed Coat: Description of an Effective Procedure Using the transparent testa 2 Gene as a Selectable Marker

Hano, Christophe; Drouet, Samantha; Lainé, Éric

doi:10.1007/978-1-0716-0751-0_17

Cited by 7 publications

(1 citation statement)

References 16 publications

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“…The transparent testa 2 (TT2) gene might be a positive control for genes that are expressed in seeds (seed coat). Seed bolls (containing seeds) used for TRV-mediated VIGS of TT2 gene showed a result of testa depigmentation with a silencing efficiency around 35% in flax seeds (Linum usitatissimum L.) (Hano et al, 2020). Only with an optimized positive control for each specific silenced region (organ) can a VIGS system provide the best silencing efficiency for application studies in that species.…”

Section: Positive Controlsmentioning

confidence: 99%

A Methodological Advance of Tobacco Rattle Virus-Induced Gene Silencing for Functional Genomics in Plants

et al. 2021

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As a promising high-throughput reverse genetic tool in plants, virus-induced gene silencing (VIGS) has already begun to fulfill some of this promise in diverse aspects. However, review of the technological advancements about widely used VIGS system, tobacco rattle virus (TRV)-mediated gene silencing, needs timely updates. Hence, this article mainly reviews viral vector construction, inoculation method advances, important influential factors, and summarizes the recent applications in diverse plant species, thus providing a better understanding and advice for functional gene analysis related to crop improvements.

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Section: Positive Controlsmentioning

confidence: 99%

A Methodological Advance of Tobacco Rattle Virus-Induced Gene Silencing for Functional Genomics in Plants

et al. 2021

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The reverse genetic as a potential of virus‐induced gene silencing in tomato biology

Tang,

Wei,

Chen

et al. 2024

Food Frontiers

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In the realm of plant genomics, virus‐induced gene silencing (VIGS) technology emerges as a potent tool, employing a reverse genetic strategy to elucidate plant gene functions. Recognized for its simplicity, cost‐effectiveness, and broad applicability, VIGS facilitates the exploration of novel genes in vegetable crops and unveils mechanisms underlying disease resistance and stress response. Moreover, it offers vital support for crop enhancement and molecular breeding. In the context of tomato biology, VIGS holds promise for transformative advancements, spanning from genomics and variety improvement to molecular breeding. This review comprehensively analyzes the pivotal breakthroughs achieved in tomato physiology through global applications of VIGS and explores its strengths and limitations. Future prospects suggest VIGS's pivotal role in reshaping tomato biology, modulating secondary metabolism, and bolstering stress resilience. By delineating diverse applications of VIGS technology, this review fosters innovation in tomato research, opening new vistas for its utilization in plant gene functional analysis.

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On “The Most Useful” Oleaginous Seeds: Linum usitatissimum L., A Genomic View with Emphasis on Important Flax Seed Storage Compounds

et al. 2021

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Virus-Induced Gene Silencing (VIGS) in Flax (Linum usitatissimum L.) Seed Coat: Description of an Effective Procedure Using the transparent testa 2 Gene as a Selectable Marker

Cited by 7 publications

References 16 publications

A Methodological Advance of Tobacco Rattle Virus-Induced Gene Silencing for Functional Genomics in Plants

A Methodological Advance of Tobacco Rattle Virus-Induced Gene Silencing for Functional Genomics in Plants

The reverse genetic as a potential of virus‐induced gene silencing in tomato biology

On “The Most Useful” Oleaginous Seeds: Linum usitatissimum L., A Genomic View with Emphasis on Important Flax Seed Storage Compounds

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