Practical Plant Virology 1998
DOI: 10.1007/978-3-642-72030-7_44
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Virus Purification Protocols

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Cited by 4 publications
(7 citation statements)
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“…A standard transmission protocol of BBTV was performed using aphids from a single colony and the BBTV reference isolate. Aphid transmission of BBTV followed the protocol of Thomas and Dietzgen (1991) with a few modifications (Dijkstra and Jager, 1998; Anhalt and Almeida, 2008). Wingless aphids were transferred into a sterilized closed container and starved for four hours.…”
Section: Methodsmentioning
confidence: 99%
“…A standard transmission protocol of BBTV was performed using aphids from a single colony and the BBTV reference isolate. Aphid transmission of BBTV followed the protocol of Thomas and Dietzgen (1991) with a few modifications (Dijkstra and Jager, 1998; Anhalt and Almeida, 2008). Wingless aphids were transferred into a sterilized closed container and starved for four hours.…”
Section: Methodsmentioning
confidence: 99%
“…A 1 ml aliquot of the purified protein was re-suspended in 100 μl of Laemmli buffer, boiled for 5 min and 20 μl aliquots were loaded onto 12% polyacrylamide gel containing SDS ( Laemmli, 1970 ). The protein purified from the columns was subjected to plate-trapped antigen ELISA (PTA-ELISA), as described previously ( Dijkstra and de Jager, 1998 ) using anti-His-Tag as the first antibody and a universal horseradish peroxidase (HRP)-conjugated antibody as the secondary antibody. Finally, 3,3′-diaminobenzidine (DAB) was added as substrate of HRP to visualize the reactions.…”
Section: Methodsmentioning
confidence: 99%
“…Efficiency of the anti-GFLV MP antibodies was examined by PTA-ELISA following Dijkstra and de Jager (1998) and DAS-ELISA ( Clark and Adams, 1977 ) to detect the purified MP and 12 samples of GFLV-infected plant tissues.…”
Section: Methodsmentioning
confidence: 99%
“…with tobacco mosaic virus (TMV) variants, so he developed the mechanical inoculation assay [ 9 ]. The procedure of mechanical inoculation and different factors that might increase the success of inoculation have been described [ 10 , 11 , 12 ]. Furthermore, the local lesion phenomenon and its role in plant resistance have been reviewed [ 8 , 13 ].…”
Section: Introductionmentioning
confidence: 99%