Virus-templated FRET platform for the rational design of ratiometric fluorescent nanosensors

Wu, Yehong; Lin, Yuan; Wang, Qin

doi:10.1039/c5cc02866c

Cited by 32 publications

(28 citation statements)

References 40 publications

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“…25 The FRET donor (fluorescein) and acceptor (rhodamine) were attached to adamantane for complexation within cyclodextrin. This approach gave a broader range of pH measurements (5.0 to 8.4), 26 than direct conjugation to amines on the phage surface (pH 6.4–7.4). 27 …”

Section: Applications Of Modified Phagementioning

confidence: 99%

Chemically Modifying Viruses for Diverse Applications

Mohan

Weiss

2016

ACS Chem. Biol.

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Long fascinating to biologists, viruses offer nanometer-scale benchtops for building molecular-scale devices and materials. Viruses tolerate a wide-range of chemical modifications including reaction conditions, pH values, and temperatures. Recent examples of non-genetic manipulation of viral surfaces have extended viruses into applications ranging from biomedical imaging, drug delivery, tissue regeneration, and biosensors to materials for catalysis and energy generation. Chemical reactions on the phage surface include both covalent and non-covalent modifications, including some applied in conjunction with genetic modifications. Here, we survey viruses chemically augmented with capabilities limited only by imagination.

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Section: Applications Of Modified Phagementioning

confidence: 99%

Chemically Modifying Viruses for Diverse Applications

Mohan

Weiss

2016

ACS Chem. Biol.

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“…6 Inspired by these natural architectures, novel artificial light-harvesting systems attempting to replicate the efficiency of the natural systems are being explored for potential use as light collectors and energy funnels in solar energy conversion systems. 7,8 FRET also plays an important role in nanosensors applications, 9,10 and can be used to tune colour composition in white-light-emitting structures. 11 In many of these applications the limited range of R DA may pose restrictions on the design and efficiency of the systems.…”

Section: Introductionmentioning

confidence: 99%

Selective turn-on and modulation of resonant energy transfer in single plasmonic hybrid nanostructures

et al. 2017

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Förster resonant energy transfer (FRET) is a nonradiative process by which the energy of light absorbed by a donor molecule is transferred to an acceptor molecule over a distance of several nanometers. FRET plays a crucial role in photosynthesis and nature-inspired artificial light-harvesting systems that are being explored for use in energy conversion applications. Localized plasmons of metal nanoparticles can potentially lead to a significant increase of FRET efficiency and effective donor-acceptor distance. Here, we prepare hybrid nanostructures composed of a gold nanorod and donor and acceptor molecules covalently attached to its surface, and study them on the level of a single nanoparticle by simultaneous dark-field scattering, fluorescence imaging and spectroscopy. The single-particle approach enables selective excitation of the longitudinal plasmon of the gold nanorod by polarization of the excitation light. The emission intensity of the acceptor molecules can be controllably and reversibly modulated over a wide range by the polarization angle, thus enabling a selective turn-on of the FRET process and control over the emission color of the hybrid nanostructure. Numerical simulations show that the interactions of the donor and acceptor molecules with the plasmon lead to an increase of the energy transfer efficiency by a factor of ∼65. These findings represent the concept of a novel colour switching approach and could pave the way for innovative applications in optoelectronics and nanophotonics.

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“…NMR studies suggested that the ZnO‐binding motif provides eleven possible interaction points to ZnO,[4b] three sites per histidine and one per serine residue, respectively. The surface expression of the ZnO‐binding peptide as N‐terminal fusion to the p8 major coat protein of M13 enabled the spatially defined presentation in nanometer dimension, similar to the distance of ≈3.2 and ≈2.4 nm, respectively, of two neighboring p8 N‐termini resulting in a high functional density of ≈130 000 peptides per µm 2 . The aligned M13‐p44 templates showed a similar roughness of ≈4 nm rms like the M13 wt template (Table ).…”

Section: Resultsmentioning

confidence: 99%

Macroscopic Properties of Biomimetic Ceramics Are Governed by the Molecular Recognition at the Bioorganic–Inorganic Interface

Kilper

Facey

Burghard

et al. 2018

Adv Funct Materials

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Bioinspired materials design aims for high-performing composite materials based on natural biomineralization processes and biomineral architectures.A key component to the research is the bioorganic-inorganic interface, one of the most crucial parameters for controlling the material properties. In this study, genetically engineered phages expressing an inorganic-binding peptide for the molecular recognition of a ceramic material is exploited to generate thin film multilayer assemblies, with the phage template as minority component. The bioorganic-inorganic interface in the ceramic (zinc oxide, ZnO) multilayer systems is strengthened by the ZnO-binding motif HSSHH of a peptide to increase Young's modulus and hardness. Applying a point-mutated version of the peptide, DSSHH, which modulates the interface forces, shows an increased fracture toughness without deteriorating the Young's modulus and the hardness. Molecular matching of the organic phase and its modulation in order to form a specific interface is shown to be important in controlling material properties like in natural biominerals. With this tool in hand, it is not only possible to imitate the structure of biominerals but also to genetically control the molecular recognition of bioorganic molecules to induce macroscopic effects in synthetic composite materials.

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Virus-templated FRET platform for the rational design of ratiometric fluorescent nanosensors

Abstract: We report here the construction of a bacteriophage M13-templated supramolecular nanosystem, i.e. M13-β-CD/Ada-FITC/Ada-RhB, which can be used as effective ratiometric fluorescent sensors for intracellular sensing.

Cited by 32 publications

References 40 publications

Chemically Modifying Viruses for Diverse Applications

Chemically Modifying Viruses for Diverse Applications

Selective turn-on and modulation of resonant energy transfer in single plasmonic hybrid nanostructures

Macroscopic Properties of Biomimetic Ceramics Are Governed by the Molecular Recognition at the Bioorganic–Inorganic Interface

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