Virus tolerance and recovery from viral induced-symptoms in plants are associated with transcriptome reprograming

Louis, Bengyella; Waikhom, Sayanika Devi; Allie, Farhahna; Rey, Chrissie

doi:10.1007/s11103-015-0362-6

Cited by 28 publications

(29 citation statements)

References 62 publications

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“…Recently, it was reported that CMD-susceptible cassava cultivars that displayed no recovery or showed a weak recovery, accumulate higher levels of viral small interfering RNAs (vsiRNAs) compared with genotypes that exhibited a strong recovery (Bengyella et al, 2015; Patil and Fauquet, 2015a; Kuria et al, 2016; Rogans et al, 2016). While these observations provide the first insights into small RNA (sRNA) profiles of CMD recovered and non-recovered phenotypes, important questions remain.…”

Section: Reversionmentioning

confidence: 99%

The Search for Resistance to Cassava Mosaic Geminiviruses: How Much We Have Accomplished, and What Lies Ahead

Fondong

2017

Front. Plant Sci.

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The cassava mosaic disease (CMD), which occurs in all cassava growing regions of Africa and the Indian subcontinent, is caused by cassava mosaic geminiviruses (CMGs). CMGs are considered to be the most damaging vector-borne plant pathogens. So far, the most successful approach used to control these viruses has been the transfer of a polygenic recessive resistance locus, designated CMD1, from wild cassava to cassava cultivars. Further progress in harnessing natural resistance to contain CMGs has come from the discovery of the dominant monogenic resistance locus, CMD2, in some West African cassava cultivars. CMD2 has been combined with CMD1 through genetic crosses. Because of the limitations of the cassava breeding approach, especially with regard to time required to produce a variety and the loss of preferred agronomic attributes, efforts have been directed toward the deployment of genetic engineering approaches. Most of these approaches have been centered on RNA silencing strategies, developed mainly in the model plant Nicotiana benthamiana. Early RNA silencing platforms assessed for CMG resistance have been use of viral genes for co-suppression, antisense suppression or for hairpin RNAs-mediated gene silencing. Here, progress and challenges in the deployment of these approaches in the control of CMGs are discussed. Novel functional genomics approaches with potential to overcome some of the drawbacks of the current strategies are also discussed.

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Section: Reversionmentioning

confidence: 99%

The Search for Resistance to Cassava Mosaic Geminiviruses: How Much We Have Accomplished, and What Lies Ahead

Fondong

2017

Front. Plant Sci.

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“…Recovery merupakan peristiwa pemulihan pada tanaman terinfeksi virus. Fenomena recovery dicirikan oleh adanya gejala infeksi awal yang parah, kemudian semakin menurun hingga hilang atau tidak bergejala pada daun yang baru berkembang (Bengyella et al, 2015). Lebih lanjut, Hagen et al (2008) Titer virus pada tiap varietas mentimun yang diuji diestimasi berdasarkan pengukuran nilai absorbansi ELISA (Tabel 2).…”

Section: Infeksi Sqmv Pada Lima Varietas Mentimununclassified

Deteksi Squash mosaic virus pada Lima Varietas Mentimun (Cucumis sativus L.)

Purba¹,

Lestari²,

Nurhaelena³

et al. 2017

J Hort Indonesia

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Squash mosaic virus (SqMV) is a seed-borne pathogen which infect many Cucurbitaceae crops. Infection of SqMV has been reported from several vegetable growing areas in Indonesia. The objective of this research was to determine the percentage of seed-borne SqMV on five cucumber varieties i.e. 'Jupiter', 'Venus', 'Japan File', 'Vario', and 'Calista' and the effect of SqMV infection on mosaic disease development. Five cucumber varieties were mechanically inoculated with SqMV, followed by observation on symptom development, incubation period, and disease incidence. Seedborne virus was detected by Dot Immunobinding Assay (DIBA) and indirect Enzyme-linked immunosorbent assay (ELISA) methods following growing-on test. The plants showed varied symptoms including green mosaic, green-yellow mosaic, vein clearing, and fruit malformation. Disease severity and virus titer showed general trend, i.e. low during inflorescence period and increasing on fruiting period; with the exception on 'Japan File' which showed decreasing of disease severity since generative phase. All commercial seeds (F1) tested evidently infected by SqMV with high incidence (100%), whereas infection of SqMV on F2 seeds of 'Venus' reached 60.87%.

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“…In many cases, symptom attenuation can be seen in the upper part of the plant, called recovery (Ghoshal and Sanfaçon, ). The capability for recovery depends on the host plant species/variety–virus interaction (Bengyella et al , ; Paudel and Sanfaçon, ) and is influenced by environmental factors, such as temperature (Fondong et al , ). Recovery is also linked to gene silencing and its dependence on temperature (Szittya et al , ).…”

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confidence: 99%

Transcriptome reprogramming in the shoot apical meristem of CymRSV‐infected Nicotiana benthamiana plants associates with viral exclusion and the lack of recovery

Medzihradszky¹,

Gyula²,

Sós-Hegedűs³

et al. 2019

Molecular Plant Pathology

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Summary In some plant–virus interactions plants show a sign of healing from virus infection, a phenomenon called symptom recovery. It is assumed that the meristem exclusion of the virus is essential to this process. The discovery of RNA silencing provided a possible mechanism to explain meristem exclusion and recovery. Here we show evidence that silencing is not the reason for meristem exclusion in Nicotiana benthamiana plants infected with Cymbidium ringspot virus (CymRSV). Transcriptome analysis followed by in situ hybridization shed light on the changes in gene expression in the shoot apical meristem (SAM) on virus infection. We observed the down‐regulation of meristem‐specific genes, including WUSCHEL (WUS). However, WUS was not down‐regulated in the SAM of plants infected with meristem‐invading viruses such as turnip vein‐clearing virus (TVCV) and cucumber mosaic virus (CMV). Moreover, there is no connection between loss of meristem function and fast shoot necrosis since TVCV necrotized the shoot while CMV did not. Our findings suggest that the observed transcriptional changes on virus infection in the shoot are key factors in tip necrosis and symptom recovery. We observed a lack of GLYCERALDEHYDE 3-PHOSPHATE DEHYDROGENASE (GAPDH) expression in tissues around the meristem, which likely stops virus replication and spread into the meristem.

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Virus tolerance and recovery from viral induced-symptoms in plants are associated with transcriptome reprograming

Cited by 28 publications

References 62 publications

The Search for Resistance to Cassava Mosaic Geminiviruses: How Much We Have Accomplished, and What Lies Ahead

The Search for Resistance to Cassava Mosaic Geminiviruses: How Much We Have Accomplished, and What Lies Ahead

Deteksi Squash mosaic virus pada Lima Varietas Mentimun (Cucumis sativus L.)

Transcriptome reprogramming in the shoot apical meristem of CymRSV‐infected Nicotiana benthamiana plants associates with viral exclusion and the lack of recovery

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