VIS–NIR spectral signature and quantitative analysis of HeLa and DU145 cell line

Ghani, Khairunnisa Abd; Sudik, Suhainah; Omar, Ahmad; Mail, Mohd Hafiz; Seeni, Azman

doi:10.1016/j.saa.2019.117241

Cited by 3 publications

(5 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Ghani et al explored the visible and near infrared spectral signature and performed quantitative analysis on cultured cervical cancer (human cervix adenocarcinoma [HeLa]) and cultured prostate cancer (human prostate carcinoma [DU145]) cells. They cultured the DU145 and HeLa cells in minimum essential medium (MEM) and Dulbecco's Modified Eagle Medium (DMEM), respectively 21 …”

Section: Introductionmentioning

confidence: 99%

“…They cultured the DU145 and HeLa cells in minimum essential medium (MEM) and Dulbecco's Modified Eagle Medium (DMEM), respectively. 21 Amran et al also investigated the potential of using phenol red in the cell culture medium to detect cancer cells. In their study, they cultured DU145 cells and L929 (mouse skin fibroblast cells) in MEM, whereas the HeLa cells were cultured in DMEM.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Spectrophotometry and colorimetry profiling of pure phenol red and cell culture medium on pH variation

Raffay

Husin

Omar

2022

Coloration Technology

Self Cite

View full text Add to dashboard Cite

The application of phenol red has been widely applied in cell culture media to enable monitoring of the pH value of cell growth. However, sometimes it is difficult for a human observer to identify the colour transformation using only the naked eye. Furthermore, different people will give different interpretations of colour transformation. Thus the current study was performed to provide the spectrophotometry and colorimetry profiling of pure phenol red and cell culture media to pH variation. Pure phenol red and cell culture media were used as samples. The pH values of both types of sample were manipulated using citric acid. Spectrophotometry analysis results show that the pH value of the pure phenol red samples could be well profiled by using the wavelengths in the green light region (in the range of 500‐600 nm). The pH value of the cell culture medium samples could also be well profiled, by using wavelengths in either the green or blue light region. Colorimetry analysis results conducted using CIELab colour space indicate that the colour transformation of both samples is linear, with a high value for the coefficient of determination. This profiling proves that phenol red can be used to quantify the pH values of the samples and shows good potential to be applied in the development of spectroscopy‐based and colorimetry‐based pH sensors.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Spectrophotometry and colorimetry profiling of pure phenol red and cell culture medium on pH variation

Raffay

Husin

Omar

2022

Coloration Technology

Self Cite

View full text Add to dashboard Cite

show abstract

“…In recent years, cancer has become the leading cause of death in the world, and its incidence is still increasing significantly. [ 1 ] Cervical cancer is a common malignant tumor in women, with approximately 530,000 new cases and 270,000 deaths every year. [ 2 ] The development of cervical cancer begins with high‐risk human papillomavirus (HPV), develops from LSIL to HSIL through cervical intraepithelial neoplasia (CIN), and finally develops into cervical cancer.…”

Section: Introductionmentioning

confidence: 99%

Feature fusion combined with tissue Raman spectroscopy to screen cervical cancer

Yang

Chen

et al. 2021

J Raman Spectroscopy

View full text Add to dashboard Cite

In this experiment, we collected 45 samples of cervicitis, 29 samples of lowgrade squamous intraepithelial lesion (LSIL), 44 samples of high-grade squamous intraepithelial lesion (HSIL), 39 samples of cervical squamous cell carcinoma, and 38 cases of cervical adenocarcinoma. After preprocessing of the Raman spectral data, partial least squares (PLS) was used to reduce the dimensionality, and then extreme gradient boosting (XGBoost) was used for feature selection to obtain the first 30-dimensional features. The preprocessed Raman spectral data also used a fast Fourier transform (FFT) to obtain ampli-

show abstract

“…Early, accurate and speedy detection of HeLa cells and the reoccurrence of small size infection after initial treatment, plays a vital role in the treatment of cervical cancer. [28] Based on the THz pulse transient measurement technology using the tilt wave front of the grating pulse, a new type of THz-TDS is designed. [29] We use LiNbO 3 crystal for THz wave generation.…”

Section: Introductionmentioning

confidence: 99%

“…Early, accurate and speedy detection of HeLa cells and the reoccurrence of small size infection after initial treatment, plays a vital role in the treatment of cervical cancer. [ 28 ]…”

Section: Introductionmentioning

confidence: 99%

Detection of living cervical cancer cells by transient terahertz spectroscopy

Shi

Wang

Hou

et al. 2020

Journal of Biophotonics

View full text Add to dashboard Cite

The photonic energy of terahertz wave is in the same order of magnitude as the rotational and vibrational energy levels of organic and biological macromolecules, so it has unique advantages in detecting cells and biological macromolecules. However, in the life environment, the dynamic time scale of cell-environment interaction and structural conformation change of biological macromolecules are within picosecond to millisecond, and water has strong absorption to terahertz wave, which has become the bottleneck problem for the detection of cells and biological macromolecules by terahertz technology. In this article, we developed a set of terahertz single measurement system based on the tilt wave front of grating pulse technique. The system was employed for the terahertz detection of trace living cervical cancer cells. We achieved transient detection of the terahertz pulse time-domain waveform of the living HeLa cells. The characteristic absorption peaks were identified by Lambert-Beer law, respectively, at 0.49, 0.71, 1.04, 1.07, 1.26 and 1.37 THz. The absorbance is proportional to the cell concentration.

show abstract

VIS–NIR spectral signature and quantitative analysis of HeLa and DU145 cell line

Cited by 3 publications

References 62 publications

Spectrophotometry and colorimetry profiling of pure phenol red and cell culture medium on pH variation

Spectrophotometry and colorimetry profiling of pure phenol red and cell culture medium on pH variation

Feature fusion combined with tissue Raman spectroscopy to screen cervical cancer

Detection of living cervical cancer cells by transient terahertz spectroscopy

Contact Info

Product

Resources

About