Viscerotopic representation of the subdiaphragmatic tracts of the digestive apparatus within the vagus complex in the sheep

Chiocchetti, Roberto; Clavenzani, Paolo; Barazzoni, Anna Maria; Grandis, Annamaria; Bombardi, Cristiano; Costerbosa, Giovanna Lalatta; Petrosino, G.; Avoni, Giulia Bompadre; Bortolami, R.

doi:10.1016/s0006-8993(02)03836-2

Cited by 14 publications

(15 citation statements)

References 12 publications

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“…In fact, sensory vagal terminations scattered in the substantia reticularis between the NPNV and the nucleus ambiguus in the ventrolateral medulla were observed after injection of CTB-HRP tracer into the DVG (also termed nodose ganglia) of lambs (26). Furthermore, vagal efferent neurons are located not only in the NPNV but also in the reticular formation of the ovine obex (27). Our classical scrapie-infected sheep exhibited a PrP Sc deposition phenotype associated with brain astrocytes, a finding which is not surprising since it has been previously observed as an early IHC deposition pattern in scrapieinfected mouse (28) and also frequently seen in the substantia reticularis in natural cases of scrapie (29).…”

Section: Discussionsupporting

confidence: 76%

Role of Palatine Tonsils as a Prion Entry Site in Classical and Atypical Experimental Sheep Scrapie

Chiocchetti

et al. 2014

J Virol

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Section: Discussionsupporting

confidence: 76%

Role of Palatine Tonsils as a Prion Entry Site in Classical and Atypical Experimental Sheep Scrapie

Chiocchetti

et al. 2014

J Virol

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“…As demonstrated by Anlauf et al (2003) in the human and by Li et al (2004) in murine small intestine (and observed in the sheep ileum by Chiocchetti et al 2003), a population of intrinsic catecholamine-containing neurons occurs in the ileum of adults. Catecholamines seem to play an antisecretory activity, and TH-IR fibres also occur in the mucosa, but their presence is sparse in comparison with that of CGRP-IR, TK-IR and VIP-IR fibres.…”

Section: Chemical Codingmentioning

confidence: 75%

“…The optimal timing for maximal cell labelling was established on the basis of our own experience with the retrograde transport of fluorescent tracers in the visceral neural pathways of sheep (Chiocchetti et al 2003). For this study, we chose a survival period of 3 weeks.…”

Section: Post-injection Survival Timementioning

confidence: 99%

Extrinsic and intrinsic sources of calcitonin gene-related peptide immunoreactivity in the lamb ileum: a morphometric and neurochemical investigation

et al. 2005

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To investigate extrinsic origins of calcitonin gene-related peptide immunoreactive (CGRP-IR) nerve fibres in the sheep ileum, the retrograde fluorescent tracer Fast Blue (FB) was injected into the ileum wall. Sections of thoraco-lumbar dorsal root ganglia (DRG) and distal (nodose) vagal ganglia showing FB-labelled neurons were processed for CGRP immunohistochemistry. The distribution of CGRP-IR in fibres and nerve cell bodies in the ileum was also studied. CGRP-IR enteric neurons were morphometrically analysed in myenteric (MP) and submucosal plexuses (SMP) of lambs (2-4 months). Sensory neurons retrogradely labelled with FB were scattered in T5-L4 DRG but most were located at the upper lumbar levels (L1-L3); only a minor component of the extrinsic afferent innervation of the ileum was derived from nodose ganglia. In the DRG, 57% of retrogradely labelled neurons were also CGRP-IR. In cryostat sections, a dense network of CGRP-IR fibres was observed in the lamina propria beneath the epithelium, around the lacteals and lymphatic follicles (Peyer's platches), and along and around enteric blood vessels. Rare CGRP-IR fibres were also present in both muscle layers. Dense pericellular baskets of CGRP-IR fibres were observed around CGRP-negative somata. The only CGRP-IR nerve cells were well-defined Dogiel type II neurons localised in the MP and in the external and internal components of the SMP. CGRP-IR neurons in the myenteric ganglia were significantly larger than those in the submucosal ganglia (mean profile areas: about 1,400 mum(2) for myenteric neurons, 750 mum(2) for submucosal neurons). About 6% of myenteric neurons and 25% of submucosal neurons were CGRP-IR Dogiel type II neurons. The percentages of CGRP-IR neurons that were also tachykinin-IR were about 9% (MP) and 42% (SMP), whereas no CGRP-IR neurons exhibited immunoreactivity for vasoactive intestinal peptide, nitric oxide synthase or tyrosine hydroxylase in either plexus. Thus, CGRP immunoreactivity occurs in the enteric nervous system of the sheep ileum (as in human small intestine and MP of pig ileum) in only one morphologically defined type of neuron, Dogiel type II cells. These are probably intrinsic primary afferent neurons.

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“…In a recent study of the viscerotopic representation of the sub-diaphragmatic tracts of the digestive apparatus within the vagus complex in the sheep, we have observed that few vagal fibres supply the ileum (Chiocchetti et al 2003a).…”

Section: Introductionmentioning

confidence: 88%

Characterisation of neurons expressing calbindin immunoreactivity in the ileum of the unweaned and mature sheep

et al. 2004

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We have identified the enteric neuron types expressing immunoreactivity for the calcium-binding protein calbindin D28k (CALB) in cryostat sections and whole-mount preparations of myenteric (MP) and submucosal (SMP) plexuses of sheep ileum. We wished to determine whether CALB-IR in the sheep enteric nervous system was expressed in Dogiel type II cells, as in guinea-pig and rat ileum, and could therefore be used as a marker for intrinsic primary afferent neurons. The neurochemical coding of CALB-containing myenteric and submucosal neurons in ileum of unweaned lamb and mature sheep and its co-localisation with various neural markers was studied immunohistochemically. An antiserum against neuronal nuclear protein (NeuN) failed to detect the entire neuronal population; it was expressed only in 48% of neuron-specific enolase (NSE)-immunoreactive (NSE-IR) neurons. Human neuronal protein appeared to occur in the large majority or all neurons. Almost all CALB-IR neurons were: (1) radially multidendritic; (2) eccentric multidendritic; (3) Dogiel type II. CALB-IR occurred in 20-25% of myenteric and 65-75% of submucosal neurons in lamb and mature sheep, with higher values in mature sheep. Nearly all CALB-IR neurons were common choline acetyltransferase (cChAT)-IR, whereas only about 20% of cChAT-IR somata were CALB-IR. In lamb and mature sheep, 90% of MP CALB-IR neurons were peripheral choline acetyltransferase (pChAT)-IR. In lamb SMP, 80+/-13% of CALB-IR cells were also pChAT-IR, whereas all those in mature SMP were pChAT-IR. Fewer myenteric CALB-IR neurons exhibited tachykinin (TK) in mature sheep (49%) than in lamb (88%). This was also the case for submucosal ganglia (mature sheep, 63%; lamb, 89%). In lamb MP, 77+/-7% of CALB-IR cells were NeuN-positive. In mature sheep, 73+/-10% of CALB-IR somata were NeuN-IR, but NeuN failed to stain SMP neurons. In the MP of suckling and mature sheep, Dogiel type II CALB-IR neurons were calcitonin gene-related peptide (CGRP)-IR. In the SMP at both stages, Dogiel type II CALB-IR somata (about 50% of CALB-IR neurons) were also CGRP-IR. Only small proportions of CALB-IR neurons showed immunoreactivity for calretinin or nitric oxide synthase (NOS), although large populations of CALB and NOS neurons occurred in the ganglia. Thus, CALB is a marker of most Dogiel type II neurons in the sheep but is not confined to Dogiel II neurons. CGRP is a more selective marker of Dogiel type II neurons, being only found in this neuron type.

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Viscerotopic representation of the subdiaphragmatic tracts of the digestive apparatus within the vagus complex in the sheep

Cited by 14 publications

References 12 publications

Role of Palatine Tonsils as a Prion Entry Site in Classical and Atypical Experimental Sheep Scrapie

Role of Palatine Tonsils as a Prion Entry Site in Classical and Atypical Experimental Sheep Scrapie

Extrinsic and intrinsic sources of calcitonin gene-related peptide immunoreactivity in the lamb ileum: a morphometric and neurochemical investigation

Characterisation of neurons expressing calbindin immunoreactivity in the ileum of the unweaned and mature sheep

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