Viscoelastic properties of fibrinogen adsorbed onto poly(ethylene terephthalate) surfaces by QCM-D

Fouling properties of new biomaterials are important for the performance of a material in a biological environment. Here, a set of three supramolecular polymeric additives consisting of ureidopyrimidinone (UPy)-functionalized poly(ethylene glycol) (UPyPEG) were formulated with UPy-modified polycaprolactone into thin supramolecular material films. The antifouling properties of these material films were determined by investigation of the relation of cell adhesion and protein adsorption on these materials films. The presence of the UPyPEG additives at the surface of the films was evident by an increased hydrophilicity. Adhesion of human epithelial and endothelial cells was strongly reduced for two of the UPyPEG-containing films. Analysis of adsorption of the first three proteins from the Vroman series, albumin, γ-globulin, and fibrinogen, using quartz crystal microbalance with dissipation in combination with viscoelastic modeling, revealed that the surfaces containing the UPyPEG additives had a limited effect on adsorption of these proteins. Despite a limited reduction of protein adsorption, UPyPEG-containing mixtures were non-cell-adhesive, which shows that non-cell-adhesive properties of supramolecular polymer surfaces are not always directly correlated to protein adsorption.

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“…A likely explanation is that the proteins adsorb not end-on but orientate sidewards as suggested before for softer layers. 34 …”

Section: Resultsmentioning

confidence: 99%

Cell and Protein Fouling Properties of Polymeric Mixtures Containing Supramolecular Poly(ethylene glycol) Additives

2017

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“…The thickness of the protein film versus plasma treatment time was determined by a QCM with a dissipation unit, QCM‐D (Model E4, QSense AB, Göteborg, Sweden). The QCM measured the mass of the thin film deposited onto a quartz crystal sandwiched between two electrodes . The electrodes were connected to a power supply that caused the crystal to oscillate at its fundamental resonance frequency and several overtones.…”

Section: Methodsmentioning

confidence: 99%

Etching of Blood Proteins in the Early and Late Flowing Afterglow of Oxygen Plasma

Vesel

Kolar

Recek

et al. 2013

Plasma Process. Polym.

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Films of proteins fibrinogen and albumin are exposed to neutral reactive particles in order to study their etching rates in early and late oxygen afterglows of microwave plasma. The etching rates are determined at different pressures between 50 and 400 Pa. The etching rates for both proteins first increase with the increasing pressure, while later they reach a constant value. Furthermore, the etching rates are higher in the early than in the late afterglow. The concentration of all available species in the afterglow, i.e. neutral oxygen atoms in the ground state, neutral molecules in the first excited state, and ozone is determined either by experimental measurements or theoretically using a well‐established model. The etching rates are explained by the synergistic effects of atoms and metastable molecules.

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“…The variations in viscosity, elastic shear modulus and thickness (fitting parameters) are shown in Figs and B–D. The results show that the viscosity, shear modulus and thickness of the adsorbed layers are highly sensitive to the nature of the lipid bilayer formed on the substrate for the adsorption of hIAPP, as observed for other proteins in which these viscoelastic properties significantly depend on the nature of the substrate , suggesting that the kinetic QCM‐D data are possibly interpreted in terms of variations in viscoelastic properties of the membrane‐adsorbed protein layer.…”

Section: Discussionmentioning

confidence: 78%

Amyloid aggregation and deposition of human islet amyloid polypeptide at membrane interfaces

2014

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Amyloid deposition of human islet amyloid polypeptide (hIAPP) within the islets of Langerhans is a pathological feature of type 2 diabetes mellitus. Substantial evidence indicates that the membrane-mediated aggregation and subsequent deposition of hIAPP are linked to dysfunction and death of pancreatic b-cells, but the molecular processes of hIAPP deposition are poorly understood. In this study, we examined the membranemediated aggregation and deposition of hIAPP at supported planar lipid bilayers with and without raft components (i.e. cholesterol and sphingomyelin) to provide insight into hIAPP-induced membrane dysfunction. The adsorption of hIAPP onto the bilayers was studied using a quartz crystal microbalance with dissipation monitoring, which showed enhanced accumulation of the peptide onto the bilayer containing raft components. Microscope observations demonstrated the growth of the aggregates formed from the membrane-adsorbed hIAPP. The examination of the membrane interfaces revealed that hIAPP aggregates retained the ability to associate with the membranes during the aggregation process, resulting in insertion of the aggregates into the bilayers. We also report the inhibitory effect of insulin on the hIAPP deposition. These findings demonstrate the aggregation of hIAPP at the membrane interfaces leading to amyloid deposits associated with the membrane and suggest a role for insulin in hIAPP deposition. A presumed mechanism regulating hIAPP deposition at the membrane interfaces is discussed.

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Viscoelastic properties of fibrinogen adsorbed onto poly(ethylene terephthalate) surfaces by QCM-D

Cited by 33 publications

References 41 publications

Cell and Protein Fouling Properties of Polymeric Mixtures Containing Supramolecular Poly(ethylene glycol) Additives

Cell and Protein Fouling Properties of Polymeric Mixtures Containing Supramolecular Poly(ethylene glycol) Additives

Etching of Blood Proteins in the Early and Late Flowing Afterglow of Oxygen Plasma

Amyloid aggregation and deposition of human islet amyloid polypeptide at membrane interfaces

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