Viscosities of the ternary solution dimethyl sulfoxide/water/sodium chloride at subzero temperatures and their application in cryopreservation

Zhang, Shaozhi; Yu, Xiaoyi; Chen, Zhaojie; Chen, Guangming

doi:10.1016/j.cryobiol.2013.01.005

Cited by 8 publications

(2 citation statements)

References 39 publications

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“…Under these conditions, they are likely nucleated with nanometric ice nuclei formed during cooling or warming. The viscosity of a 40% DMSO solution increases by two orders of magnitude when the temperature drops from −30 °C to −50 °C . Close to Tg, reduction of 10 °C increases the viscosity by 3 orders of magnitude .…”

Section: Resultsmentioning

confidence: 99%

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Extended Temperature Range of the Ice-Binding Protein Activity

Sirotinskaya,

Bar Dolev,

Yashunsky

et al. 2024

Langmuir

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Ice-binding proteins (IBPs) are expressed in various organisms for several functions, such as protecting them from freezing and freeze injuries. Via adsorption on ice surfaces, IBPs depress ice growth and recrystallization and affect nucleation and ice shaping. IBPs have shown promise in mitigating ice growth under moderate supercooling conditions, but their functionality under cryogenic conditions has been less explored. In this study, we investigate the impact of two types of antifreeze proteins (AFPs): type III AFP from fish and a hyperactive AFP from an insect, the Tenebrio molitor AFP, in vitrified dimethylsulfoxide (DMSO) solutions. We report that these AFPs depress devitrification at −80 °C. Furthermore, in cases where devitrification does occur, AFPs depress ice recrystallization during the warming stage. The data directly demonstrate that AFPs are active at temperatures below the regime of homogeneous nucleation. This research paves the way for exploring AFPs as potential enhancers of cryopreservation techniques, minimizing icegrowth-related damage, and promoting advancements in this vital field.

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Section: Resultsmentioning

confidence: 99%

“…The viscosity of a 40% DMSO solution increases by two orders of magnitude when the temperature drops from −30 °C to −50 °C. 68 Close to Tg, reduction of 10 °C increases the viscosity by 3 orders of magnitude. 5 The diffusion reverses the correlation with the viscosity.…”

Section: ■ Materials and Methodsmentioning

confidence: 99%