Densities and viscosities of glycine, L-alanine, Lvaline, L-threonine, and L-arginine in aqueous solutions of (0.0, 0.2, 0.4, 0.6, 0.8, and 1.0) mol·kg −1 mannitol have been measured in the temperature range of (293.15 to 323.15) K under atmospheric pressure; then the measured densities and viscosities of experimental systems were correlated. These data were further used to calculate the apparent molar volumes (V φ ), the limiting partial molar volumes (V φ 0 ), the limiting partial molar volumes of transfer (Δ tr V φ 0 ), the viscosity Bcoefficients, and the free energies of activation per mole of solvent (Δμ 1 0≠ ) and solute (Δμ 2 0≠ ). The results were interpreted through solute−solute interaction and solute− solvent interaction on the basis of the cosphere overlap model and transition state theory.