Visual and microplate detection of aflatoxin B2 based on NaCl-induced aggregation of aptamer-modified gold nanoparticles

Luan, Yunxia; Chen, Jiayi; Xie, Gang; Li, Cheng; Hua, Ping-Rang; Ma, Zhiyong; Lu, Anxiang

doi:10.1007/s00604-014-1420-5

Cited by 41 publications

(17 citation statements)

References 28 publications

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“…In order to optimize the NaCl concentration, AuNPs were treated with different volumes of 2 M NaCl solution (0, 10,15,20,25,30,35, and 40 μL) and MOPS buffer were added to attain a total volume of 500 μL. After incubating for 5 min, A520 and A650 were recorded, and the minimum NaCl concentration that can fully induce aggregation of AuNPs was chosen.…”

Section: Optimization Of Detection Conditionsmentioning

confidence: 99%

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A label-free colorimetric progesterone aptasensor based on the aggregation of gold nanoparticles

Zhang

Xia

et al. 2016

Microchim Acta

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The authors describe an aptasensor for the detection of the gonadal hormone progesterone (P4) in aqueous solution. Gold nanoparticles (AuNPs) were coated with a P4-specific aptamer, and such particles do not aggregate in presence of NaCl due to the presence of the aptamer coating. If, however, progesterone is added, it will bind to the aptamer and release it from the surface. The uncoated AuNPs, on addition of NaCl, will aggregate and a color change from red (520 nm) to blue (650 nm) can be visually detected or photometrically quantified. The ratio of the absorbances at 650 and 520 nm is linearly related to the P4 concentration in the range from 2.6 to 800 nM. The complete detection range extends from 2.6 to 1400 nM, and the detection limit is 2.6 nM. Water containing various potential interferents, as well as tap water and urine, were spiked with P4 and the recoveries of P4 are in the range of 89.7-117.5%, 84.4-115.0% and 94.7-118.8%, respectively. This assay has a large potential with respect to the visual and instrumental determination of P4 in aquatic environment and urine.

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Section: Optimization Of Detection Conditionsmentioning

confidence: 99%

“…Thus, several metal ions and small organic molecules have been detected through the unmodified AuNP-based colorimetric assays [22]. Additionally, NaCl have been employed to control the aggregation of AuNPs, which received excellent results [23][24][25].…”

Section: Introductionmentioning

confidence: 99%

A label-free colorimetric progesterone aptasensor based on the aggregation of gold nanoparticles

Zhang

Xia

et al. 2016

Microchim Acta

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“…After that, the target-induced conformational change exposes the AuNPs to NaCl-induced aggregation leading to a colour change. The linear dynamic range and detection sensitivity were found to be 0.025–100 µg/L and 0.025 µg/L of AFB1, respectively [77,78]. Seok et al reported another colorimetric assay based on AFB1-induced DNA structural changes and peroxidase mimicking DNAzyme.…”

Section: Label-free Aptasensors For Myctotoxin Determinationmentioning

confidence: 99%

Label-Free Aptasensors for the Detection of Mycotoxins

Rhouati

Catanante

Nunes

et al. 2016

Sensors

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Various methodologies have been reported in the literature for the qualitative and quantitative monitoring of mycotoxins in food and feed samples. Based on their enhanced specificity, selectivity and versatility, bio-affinity assays have inspired many researchers to develop sensors by exploring bio-recognition phenomena. However, a significant problem in the fabrication of these devices is that most of the biomolecules do not generate an easily measurable signal upon binding to the target analytes, and signal-generating labels are required to perform the measurements. In this context, aptamers have been emerged as a potential and attractive bio-recognition element to design label-free aptasensors for various target analytes. Contrary to other bioreceptor-based approaches, the aptamer-based assays rely on antigen binding-induced conformational changes or oligomerization states rather than binding-assisted changes in adsorbed mass or charge. This review will focus on current designs in label-free conformational switchable design strategies, with a particular focus on applications in the detection of mycotoxins.

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“…2), the present study reports the synthesis of desert rose-like GNPs (DR-GNPs), their characterization by means of UV-vis absorption spectroscopy, transmission electron microscopy (TEM) and dynamic light scattering (DLS) and their use as labels for ICST. Although other ligands have been reported, such as for example aptamers [14][15], the large majority of GNP-based ICSTs reported in the literature and commercially available use antibodies as recognition elements [16][17] and most frequently the labeling of antibodies with GNPs is achieved through passive adsorption of proteins on their surfaces. Therefore, a preliminary study on the passive adsorption of antibodies on DR-GNPs as a function of the pH and the amount of antibody (Ab) used for conjugation with DR-GNP was conducted.…”

Section: Introductionmentioning

confidence: 99%

Multicolor immunochromatographic strip test based on gold nanoparticles for the determination of aflatoxin B1 and fumonisins

et al. 2017

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Graphical abstractBlue desert rose-like gold nanoparticles (DR-GNPs) were synthesized, characterized and applied as label for the ImmunoChromatographic Strip Test (ICST) technique, in which red spherical GNPs (sGNPs) are usually employed. The combined use of the blue DR-GNP and red s-GNPs allowed developing of an intuitive multicolor ICST for the simultaneous detection of Aflatoxin B1 and AbstractDesert rose-like gold nanoparticles (DR-GNPs) that exhibited a blue color due to the plasmon resonance band at 620 nm were prepared. The blue DR-GNPs were obtained through a seeding growth approach and characterized by UV-vis spectroscopy, transmission electron microscopy and dynamic light scattering. The DR-GNPs had a hydrodynamic diameter of ~72 nm and were used as labels for the antibody in an immunochromatographic strip test (ICST). Although the particular shape of DR-GNPs and their higher surface area with respect to spherical GNPs, they demonstrated to be effective as antibody labels. A multicolor ICST for aflatoxin B1 and fumonisins was developed that employs both blue DR-and red spherical GNPs. The multicolor ICST allows for simultaneous rapid determination of the two mycotoxins in maize flour, with visual cut-off levels at 2 µg kg -1 for aflatoxin B1 and 1000 µg kg -1 for fumonisins.

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Visual and microplate detection of aflatoxin B2 based on NaCl-induced aggregation of aptamer-modified gold nanoparticles

Cited by 41 publications

References 28 publications

A label-free colorimetric progesterone aptasensor based on the aggregation of gold nanoparticles

A label-free colorimetric progesterone aptasensor based on the aggregation of gold nanoparticles

Label-Free Aptasensors for the Detection of Mycotoxins

Multicolor immunochromatographic strip test based on gold nanoparticles for the determination of aflatoxin B1 and fumonisins

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