Visual detection of kanamycin with DNA-functionalized gold nanoparticles probe in aptamer-based strip biosensor

Ou, Ying; Jin, Xin; Liu, Jing; Tian, Yaping; Zhou, Nandi

doi:10.1016/j.ab.2019.113432

Cited by 42 publications

(23 citation statements)

References 32 publications

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“…AuNPs's size-dependent surface resonance (SPR) absorption located at about 518 nm has been employed widely as a colorimetric sensor for many molecules [ 24 , 28 , 29 ]. The color of the gold solution reflects the degree of aggregation of AuNPs in suspension.…”

Section: Resultsmentioning

confidence: 99%

“…Aptamers are single-stranded oligonucleotides (ssDNA or ssRNA). ssDNA with random coil structures could adsorb on the surface of AuNPs and protect AuNPs from salt-induced aggregation [ 24 ]. Because of distinctive adsorptive properties of the aptamers toward AuNPs, the binding of the target or conformational changes in the DNA result in assembly of the AuNPs to produce colorimetric signal [ 25 ].…”

Section: Introductionmentioning

confidence: 99%

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Aptamer-Based Colorimetric Probe for trans-Zeatin Detection Using Unmodified Gold Nanoparticle

Sun

Zhang

Wang

2020

International Journal of Analytical Chemistry

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Trans-Zeatin is the major active phytohormone in immature corn kernels. Herein, a highly sensitive, good selective and simple aptamer-based colorimetric method for the detection of trans-zeatin was constructed. The selected aptamer sequence binds with trans-zeatin and induces a duplex-to-aptamer structure switching. The gold nanoparticles (AuNPs) solution is stable with high-concentration salt, which is protected by red complementary DNA. In the absence of trans-zeatin, the color of AuNPs changed from red to blue because aptamer DNA and complementary DNA form double-stranded DNA. Thus, the ratio of absorbance intensities (A522/A650) of AuNPs is changed with the concentration of trans-zeatin. The color change could be observed by the naked eye. The linear range of this method covers a large variation of trans-zeatin concentration from 0.05 to 0.75 μM. The detection limit is 0.037 μM. Moreover, this method was applied successfully to detect trans-zeatin in real plant samples.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Aptamer-Based Colorimetric Probe for trans-Zeatin Detection Using Unmodified Gold Nanoparticle

Sun

Zhang

Wang

2020

International Journal of Analytical Chemistry

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“…It is more sensitive and accurate than other field-applicable assays, which are usually based on isothermal amplification alone 11 . The strip can be kept in dry environment at 4°C for at least 3 months, as previously reported, making it suitable for commercial use [39][40][41] . Despite the robust results, the high cost of commercial strips limits their broad application for ASFV detection.…”

Section: Discussionmentioning

confidence: 96%

Rapid detection of African swine fever virus using Cas12a-based portable paper diagnostics

Chen

et al. 2020

Cell Discov

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African swine fever virus (ASFV) is a dsDNA virus responsible for a severe, highly contagious, and lethal disease affecting both domestic and wild pigs. ASFV has brought enormous economic loss to a number of countries, and effective vaccine and therapy are still lacking. Therefore, a rapid, sensitive, and field-deployable detection of ASFV is important for disease surveillance and control. Herein, we developed a Cas12a-mediated portable paper assay to rapidly and precisely detect ASFV. We identified a robust set of crRNAs that recognized the highly conserved region of essential ASFV genes. The Cas12a-mediated detection assay showed low tolerance for mismatch mutations, and no cross-reactivity against other common swine pathogens. We further developed a paper-based assay to allow instrument-free detection of ASFV. Specifically, we applied gold nanoparticle-antibody conjugate to engineer homemade strips and combined it with Cas12a-mediated ASFV detection. This portable paper, instrument-free diagnostics, faithfully detected ASFV in swine samples, showing comparable sensitivity to the traditionally instrumentdependent qPCR method. Taking together, we developed a highly sensitive, instant, and economic Cas12a-mediated paper diagnostics of ASFV, with a great application potential for monitoring ASFV in the field.

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“…The assay does not need several incubations and washing steps [ 46 ]. In a similar study using aptamer-AuNPs, the magnetic microspheres were used to detect kanamycin in the form of faded band at test region on a nitrocellulose strip [ 47 ]. Non-enzymatic hybridization chain reaction–based nanosensor has been used for the detection of kanamycin in milk.…”

Section: Antibiotic Detectionmentioning

confidence: 99%

“…DNA-functionalized AuNPs are useful for detection of genotoxic, 2-anthramine, acridine orange, and 2-naphthylamine in water samples from Henan province, China [ 138 ]. DNA-functionalized AuNPs have been used for the sensing of kanamycin in honey, milk, and milk powder samples [ 47 ]. Probe-functionalized Au core-Au shell NPs have been used for the kanamycin detection in milk samples [ 139 ].…”

Section: Practical Application Of Nanosensors and Future Prospectivementioning

confidence: 99%

Application of DNA-Nanosensor for Environmental Monitoring: Recent Advances and Perspectives

Kumar

Guleria

2020

Curr Pollution Rep

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Visual detection of kanamycin with DNA-functionalized gold nanoparticles probe in aptamer-based strip biosensor

Cited by 42 publications

References 32 publications

Aptamer-Based Colorimetric Probe for trans-Zeatin Detection Using Unmodified Gold Nanoparticle

Aptamer-Based Colorimetric Probe for trans-Zeatin Detection Using Unmodified Gold Nanoparticle

Rapid detection of African swine fever virus using Cas12a-based portable paper diagnostics

Application of DNA-Nanosensor for Environmental Monitoring: Recent Advances and Perspectives

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