Visual genotyping of thalassemia by using pyrrolidinyl peptide nucleic acid probes immobilized on carboxymethylcellulose-modified paper and enzyme-induced pigmentation

Jirakittiwut, Nuttapon; Munkongdee, Thongperm; Wongravee, Kanet; Sripichai, Orapan; Fucharoen, Suthat; Praneenararat, Thanit; Vilaivan, Tirayut

doi:10.1007/s00604-020-4197-8

Cited by 13 publications

(6 citation statements)

References 35 publications

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“…The acpcPNA processes desirable characteristics of binding in antiparallel orientation and low self-hybridization, demonstrating a stronger affinity and higher sequence specificity binding over Nielsen's original PNA. As a result, several reports have extensively utilized the acpcPNA as a probe for DNA biosensors incorporating analytical detection such as colorimetric [53,82,83] and electrochemical assay. [65,67,84]…”

Section: Type Of Probes Used In Dna Detectionmentioning

confidence: 99%

Point‐of‐Care Testing (POCT) Devices for DNA Detection: A Comprehensive Review

Mahardika,

Naorungroj,

Khamcharoen

et al. 2023

Advanced NanoBiomed Research

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DNA chips play a crucial role in point‐of‐care diagnostics by enabling rapid and accurate detection of genetic information. These chips offer high sensitivity and selectivity, allowing for the identification of specific DNA sequences associated with diseases and pathogens. Integration into lab‐on‐chip platforms streamlines and miniaturizes diagnostic workflows, paving the way for cost‐effective, portable, and user‐friendly testing devices that can revolutionize healthcare delivery. In this review, a comprehensive description of the platforms utilized in DNA analysis, including microfluidic devices and integrated DNA chips, is provided. It explores the selection and immobilization of DNA probes for improved selectivity. Additionally, it covers diverse detection techniques such as optical detection (colorimetry and fluorescence) and electrochemical techniques. In these discussions, it is aimed to provide a thorough understanding of the current state of the art in DNA biosensor‐integrated lab‐on‐chip technology for point‐of‐care testing. The continued advancements in DNA chip technology hold immense promise for the development of next‐generation point‐of‐care diagnostics, where integrated sample preparation and rapid results generation can further enhance patient outcomes and contribute to the effective management of diseases.

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Section: Type Of Probes Used In Dna Detectionmentioning

confidence: 99%

Point‐of‐Care Testing (POCT) Devices for DNA Detection: A Comprehensive Review

Mahardika,

Naorungroj,

Khamcharoen

et al. 2023

Advanced NanoBiomed Research

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show abstract

“…259 Thus, acpcPNA probes have been extensively used as the key recognition element in paper-or chip-based electrochemical and optical nucleic acid sensing devices for point-of-care applications. [260][261][262][263][264][265][266][267] In many cases, the PNAÁDNA hybridization resulted in the change of electrical properties such as capacitance and thus no labeling or other signal transduction mechanism was required. 268,269 While acpcPNA probes have been primarily used for DNA detection, satisfactory performance in the electrochemical detection of RNA was successfully demonstrated.…”

Section: Modified Pna For Nucleic Acid Biosensing and Bioimagingmentioning

confidence: 99%

Perspectives on conformationally constrained peptide nucleic acid (PNA): insights into the structural design, properties and applications

Suparpprom

Vilaivan

2022

RSC Chem. Biol.

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“…In addition, fluorophore-labeled PNA probes are able to quantitatively and specifically detect multiplexed miRNAs in living cancer cells when conjugated with the nano metal–organic framework (NMOF) vehicle, and the release of PNAs from the NMOF would lead to the recovery of fluorescence ( Wu et al, 2015 ). Innovatively, the interaction between immobilized PNA probes and DNA targets leads to enzyme-catalyzed pigmentation, allowing for simple visual read-out with up to 100% accuracy ( Jirakittiwut et al, 2020 ).…”

Section: The Application Of Nucleic Acid Probe–based Fluorescent Sensing In Cancer Diagnosis and Treatmentmentioning

confidence: 99%

The Application of Nucleic Acid Probe–Based Fluorescent Sensing and Imaging in Cancer Diagnosis and Therapy

Huang

Wang

et al. 2021

Front. Chem.

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It is well known that cancer incidence and death rates have been growing, but the development of cancer theranostics and therapeutics has been a challenging work. Recently, nucleic acid probe–based fluorescent sensing and imaging have achieved remarkable improvements in a variety of cancer management techniques, credited to their high sensitivity, good tolerance to interference, fast detection, and high versatility. Herein, nucleic acid probe–based fluorescent sensing and imaging are labeled with advanced fluorophores, which are essential for fast and sensitive detection of aberrant nucleic acids and other cancer-relevant molecules, consequently performing cancer early diagnosis and targeted treatment. In this review, we introduce the characteristics of nucleic acid probes, summarize the development of nucleic acid probe–based fluorescent sensing and imaging, and prominently elaborate their applications in cancer diagnosis and treatment. In discussion, some challenges and perspectives are elaborated in the field of nucleic acid probe–based fluorescent sensing and imaging.

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Visual genotyping of thalassemia by using pyrrolidinyl peptide nucleic acid probes immobilized on carboxymethylcellulose-modified paper and enzyme-induced pigmentation

Cited by 13 publications

References 35 publications

Point‐of‐Care Testing (POCT) Devices for DNA Detection: A Comprehensive Review

Point‐of‐Care Testing (POCT) Devices for DNA Detection: A Comprehensive Review

Perspectives on conformationally constrained peptide nucleic acid (PNA): insights into the structural design, properties and applications

The Application of Nucleic Acid Probe–Based Fluorescent Sensing and Imaging in Cancer Diagnosis and Therapy

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