Visual microarray detection for human IgE based on silver nanoparticles

Li, Zhoumin; Li, Zhonghui; Niu, Qiqi; Li, Hui; Vuki, Maika; Xu, Danke

doi:10.1016/j.snb.2016.07.142

Cited by 15 publications

(9 citation statements)

References 37 publications

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“…Such LOD is lower than the LOD of 0.2 ng mL -1 for IgE with fluorescent detection using an immunoprobe labelled with AuNCs [30]. Also, it compares favorably with most recently published methods for IgE determination in human serum (see Table 2) [30][31][32][33][34][35][36][37][38]. With ICP-MS detection of platinum, the linear range (IC20-IC80) extended from 0.10 ng mL -1 up to 2.6 ng mL -1 of IgE.…”

Section: Immunoassays For Ige Determination With Fluorescence and Icpmentioning

confidence: 65%

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Bimodal determination of immunoglobulin E by fluorometry and ICP-MS by using platinum nanoclusters as a label in an immunoassay

et al. 2019

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The authors describe the use of platinum nanoclusters (PtNCs) as bimodal labels in a competitive immunoassay for immunoglobulin E (IgE). Both fluorometry and inductively coupled plasmamass spectrometry (ICP-MS) are used. Optimization of the PtNCs synthesis process using lipoic acid as ligand was carried out. The time for synthesis and the effect of NaOH added to the PtNCs precursor mixture was optimized with the aim to obtain PtNCs with strong fluorescence and low size dispersity. Maximal fluorescence was obtained at excitation/emission wavelengths of 455/620 nm. The average diameter (1.5 nm) and crystal structure (face-centered cubic structure) of the PtNCs were determined by HR-TEM. It was calculated that each PtNC contains 116 Pt atoms at average. Labelling of the antibody (Ab) against IgE with PtNCs was optimized in terms of recognition capabilities and fluorescence signal. A molar ratio (Ab:PtNCs) of 1:11 is found to be best. A competitive immunoassay for IgE was developed and detection was carried out by using both ICP-MS (by measuring 195 Pt) and fluorometry. The limit of detection (LOD) of the fluoroimmunoassay is 0.6 ng mL-1 of IgE. The LOD of the ICP-MS method is as low as 0.08 ng mL-1. The method was evaluated by analyzing four (spiked) serum samples by ICP-MS. No sample pretreatment excepting dilution is needed. Results compared favorably with those obtained by a commercial ELISA kit.

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Section: Immunoassays For Ige Determination With Fluorescence and Icpmentioning

confidence: 65%

“…There are no conflicts to declare. CdS nanospheres and AuNPs Electrochemiluminescence aptasensor 0.02 [36] Aptamer modified with AgNPs Visual microarray 20 [37] Polystyrene and super-paramagnetic particle complexes…”

Section: Conflict Of Interestmentioning

confidence: 99%

Bimodal determination of immunoglobulin E by fluorometry and ICP-MS by using platinum nanoclusters as a label in an immunoassay

et al. 2019

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“…In colorimetric detections, nanoparticles are also frequently used as reporting agents including silver [102], gold [103,104] and carbon nanoparticles [105] and aptamer-labeled bacteriophages [106]. Aptamer-modified silver nanoparticles were used for rapid, visual IgE detection, where anti-human IgE antibodies were spotted on solid support to capture IgE and form antibody/IgE/aptamer-AgNP complex.…”

Section: Ige Detection With Aptamersmentioning

confidence: 99%

“…The method was able to detect IgE down to ca. 20 ng/mL (105 pM) in a cost effective, easy-to-use manner [102].…”

Section: Ige Detection With Aptamersmentioning

confidence: 99%

Aptamers against Immunoglobulins: Design, Selection and Bioanalytical Applications

Bognár

Gyurcsányi

2020

IJMS

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Nucleic acid aptamers show clear promise as diagnostic reagents, as highly specific strands were reported against a large variety of biomarkers. They have appealing benefits in terms of reproducible generation by chemical synthesis, controlled modification with labels and functionalities providing versatile means for detection and oriented immobilization, as along with high biochemical and temperature resistance. Aptamers against immunoglobulin targets—IgA, IgM, IgG and IgE—have a clear niche for diagnostic applications, therefore numerous aptamers have been selected and used in combination with a variety of detection techniques. The aim of this review is to overview and evaluate aptamers selected for the recognition of antibodies, in terms of their design, analytical properties and diagnostic applications. Aptamer candidates showed convincing performance among others to identify stress and upper respiratory tract infection through SIgA detection, for cancer cell recognition using membrane bound IgM, to detect and treat hemolytic transfusion reactions, autoimmune diseases with IgG and detection of IgE for allergy diseases. However, in general, their use still lags significantly behind what their claimed benefits and the plethora of application opportunities would forecast.

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“…A number of studies have reported the electrochemical sensing of IgE [4][5][6][7][8][9][10][11][12][13][14][15][16], with C, Pt, and Au being employed as electrode materials for the screen-printed electrochemical transducers used in allergen detection. Subsequent modification and functionalization of these materials was therefore aimed at promoting faster charge transfer, the stable attachment of bioreceptors, and enhancing the loading capacity of biomolecules.…”

Section: Introductionmentioning

confidence: 99%

Feasibility Study of Biosensors Based on Polymelamine‐modified Screen‐printed Carbon Electrodes

Tsai

Chang

et al. 2017

Electroanalysis

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We herein report the use of melamine and a low‐cost screen‐printed carbon electrode (SPCE) as the base matrices for the preparation of an electrochemical biosensor. Following the electrochemical polymerization of melamine, the resulting polymelamine was deposited on the SPCE surface to give layers bearing –NH2 functional groups, which allowed the attachment of anti‐IgE (immunoglobulin E) antibodies. The resulting anti‐IgE‐labeled SPCEs were then incubated with IgE solutions of various concentrations prior to analysis by chronoamperometry using Ru(NH3)63+ as an electrochemical mediator. A logarithmic relationship was observed between the chronoamperometric current and the IgE concentration between 5.3 and 530 fM (i. e. over 2 orders of magnitude). In addition, a detection limit of 0.64 fM was achieved in addition to a recovery of 114 ± 14 % for a fetal bovine serum sample spiked with 16 fM IgE. Furthermore, only a small quantity of sample was required for analysis, and the IgE assay was suitable for use in a complex serum matrix without interference. We therefore expect that this novel system will be useful for monitoring the changes in blood IgE levels during the clinical treatment of allergic asthma and rhinitis.

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Visual microarray detection for human IgE based on silver nanoparticles

Cited by 15 publications

References 37 publications

Bimodal determination of immunoglobulin E by fluorometry and ICP-MS by using platinum nanoclusters as a label in an immunoassay

Bimodal determination of immunoglobulin E by fluorometry and ICP-MS by using platinum nanoclusters as a label in an immunoassay

Aptamers against Immunoglobulins: Design, Selection and Bioanalytical Applications

Feasibility Study of Biosensors Based on Polymelamine‐modified Screen‐printed Carbon Electrodes

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