Visual Scanometric Detection of DNA through Silver Enhancement Regulated by Gold‐Nanoparticle Aggregation with a Molecular Beacon as the Trigger

Ji, Hanxu; Dong, Haifeng; Yan, Feng; Lei, Jianping; Ding, Ling; Gao, Wenchao; Ju, Huangxian

doi:10.1002/chem.201100563

Cited by 22 publications

(10 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Amine modification on PS plate and GS surfaces Functionalization of amine group on the surface of PS plate and GS was carried out with earlier described methods (Kaur and Raman Suri 2007;Ji et al 2011). The plates were treated with freshly prepared HNO 3 and H 2 SO 4 mixtures (47:53 ratio, 250 lL/well) at room temperature for 30 min in a fume hood with constant shaking.…”

Section: Synthesis Of Citrate-capped Aunpsmentioning

confidence: 99%

“…Nitro group was introduced on the benzene of PS after treated with a nitrating mixture (HNO 3 and H 2 SO 4 ) and GS surfaces were treated with piranha solution. Then the treated surfaces were functionalized with amine functional group through the condensation reaction with organosilane (APTES) (Kaur and Raman Suri 2007;Ji et al 2011). The hydrophobic and hydrophilic properties of modified and unmodified PS plate and GS surfaces were characterized using with WCA measurement method.…”

Section: Synthesis and Characterization Of Citrate-capped Aunpsmentioning

confidence: 99%

See 1 more Smart Citation

Gold nanoparticles assisted characterization of amine functionalized polystyrene multiwell plate and glass slide surfaces

et al. 2014

View full text Add to dashboard Cite

We demonstrated citrate-capped gold nanoparticles assisted characterization of amine functionalized polystyrene plate and glass slide surfaces through AuNPs staining method. The effect of AuNPs concentration on the characterization of amine modified surfaces was also studied with different concentration of AuNPs (ratios 1.0-0.0). 3-Aminopropylyl triethoxy silane has been used as amine group source for the surface modification. The interactions of AuNPs on modified and unmodified surfaces were investigated using atomic force microscopy and the dispersibility, and the aggregation of AuNPs was analyzed using UV-visible spectrophotometer. Water contact angle measurement and X-ray photoelectron spectroscopy (XPS) were used to further confirmation of amine modified surfaces. The aggregation of AuNPs in modified multiwell plate leads to the color change from red to purple and they are found to be adsorped on the modified surfaces. Aggregation and adsorption of AuNPs on the modified surfaces through the electrostatic interactions and the hydrogen bonds were revealed by XPS analysis. Remarkable results were found even in the very low concentration of AuNPs (ratio 0.2). This AuNPs staining method is simple, cost-effective, less time consuming, and required very low concentration of AuNPs. These results can be read out through the naked eye without the help of sophisticated equipments.

show abstract

Section: Synthesis Of Citrate-capped Aunpsmentioning

confidence: 99%

Section: Synthesis and Characterization Of Citrate-capped Aunpsmentioning

confidence: 99%

Gold nanoparticles assisted characterization of amine functionalized polystyrene multiwell plate and glass slide surfaces

et al. 2014

View full text Add to dashboard Cite

show abstract

“…Finally, the detection sensitivity was increased up to atomolar level through the array-based detection of DNA by the scanometric method with electrical readout. , Moreover, the scanometric method has been used to detect single nucleotide mismatch, specific RNA targets, and prostate cancer markers (microRNA). Scanometric-based biobarcode assay is used for mRNA expression analysis and Alzheimer’s disease diagnosis. ,− It is also used to carry out imaging of DNA hybridization, and high-level mismatch discrimination of anthrax lethal factor DNA, light scattering property-based homogenous detection of DNA, microarray detection of double- and triple-helix DNA binders, visual detection of DNA with molecular beacon, and analysis of DNA microarray with DNA intercalator-conjugated AuNPs were reported. − …”

Section: Introductionmentioning

confidence: 99%

Scanometric Detection of Tomato Leaf Curl New Delhi Viral DNA Using Mono- and Bifunctional AuNP-Conjugated Oligonucleotide Probes

et al. 2019

View full text Add to dashboard Cite

Scanometric detection of tomato leaf curl New Delhi viral DNA using AuNP-conjugated mono- and bifunctional oligo probes through direct DNA hybridization assay (DDH assay) and sandwich DNA hybridization assay (SDH assay) with silver enhancement was developed. Tomato leaf curl New Delhi virus (ToLCNDV) coat protein gene-specific thiol-modified ssoligo probes were used for the preparation of mono- and bifunctional AuNP-ssoligo probe conjugates (signal probes). ssDNA arrays were prepared using polymerase chain reaction (PCR), rolling circle amplification (RCA), genomic DNAs fragments, and phosphate-modified positive control/capture probes through 1-ethyl-3-(3-dimethylaminopropyl)-carbodiimide/1-methylimidazole conjugation on the amine-modified glass slide (GS) surface. In the DDH assay, signal probes were directly hybridized with ssDNA array of positive control and ToLCNDV DNA samples and the detection signals were amplified by silver enhancement. Dark black/gray colors were developed on the GS by the result of Ag enhancement, which can be visualized and discriminated by the naked eye. The images were captured using a simple flatbed scanner, and the determined amounts of signal probes were hybridized with their target DNA. Similarly, the SDH assay also performed through two rounds of hybridization between capture probes and target DNA; target DNA and signal probes followed by silver enhancement. The detection signals were found higher in the PCR sample than the RCA and genomic DNA samples because of the presence of increased copy numbers of complementary DNAs in PCR samples. Further, bifunctional AuNP-ssoligo probe shows higher intensity of detection signal than monofunctional probes because it can be hybridized with both strands of dsDNA targets. Moreover, the DDH-based scanometric method showed higher detection sensitivity than the SDH assay-based scanometric method. Overall, bifunctional signal probes showed more detection sensitivity than monofunctional probes in scanometric methods based on both DDH and SDH assays. The limit of detection of this developed scanometric method was optimized (100 zM to 100 pM concentration). Further, DDH assay-based scanometric method shows significant advantages over the SDH assay method, such as cost-effectiveness, because it requires only single probes (signal probes), less time-consuming by the need of only single-step hybridization, and higher detection sensitivity (up to zM). To the best of our knowledge, this is the first attempt made to develop a scanometric-based nanoassay method for the detection of plant viral DNA. This approach will be a remarkable milestone for the application of nanotechnology in the development of nanobiosensor for plant pathogen detection.

show abstract

“…Developing a simple, rapid, sensitive and effective DNA detection method that can be operated by the public with accessible resources especially without suitable cell lines is one of the great challenges in analytical chemistry. 3 High performance liquid chromatography, 4 ultraviolet, 5,6 uorescence, [7][8][9] electrochemistry, 6,10-12 photoelectrochemistry 13 and ECL had been used to detect DNA. Among them, nanoprobes played an important role in all these methods.…”

Section: Introductionmentioning

confidence: 99%

Fabrication of CdTe@SiO2 nanoprobes for sensitive electrogenerated chemiluminescence detection of DNA damage

Wei

Zhou

et al. 2013

Analyst

View full text Add to dashboard Cite

Detection of DNA damage is significant for the evaluation of genotoxicity of new chemicals in the early stages of its development. An electrogenerated chemiluminescence (ECL) biosensor was fabricated to detect specific sequences of DNA by using CdTe@SiO2 as nanoprobes for signal amplification. This DNA biosensor was constructed by self-assembly of an aminated capture DNA on the glass carbon electrode. DNA detection was realized by outputting a remarkable ECL signal of the CdTe@SiO2 labeled probe DNA. When the target DNA was introduced into the system, it was complementary to the probe DNA at the one-half-segment and complementary to the capture DNA at the other half-segment, resulting in the formation of a stable duplex complex. As a result, the CdTe@SiO2 labeled probe was proximate to the electrode surface and the ECL was observed. This DNA biosensor was proved to have a low detection limit (0.03 nM) and a wide dynamic range (from 0.1 nM to 2 μM). Most importantly, the sensing system could differentiate the single base mismatched DNA from the complementary DNA. It was successfully applied to study the damage to DNA caused by several genotoxicity chemicals, which was rapid, simple, reliable and sensitive compared to the classical biological methods.

show abstract

Visual Scanometric Detection of DNA through Silver Enhancement Regulated by Gold‐Nanoparticle Aggregation with a Molecular Beacon as the Trigger

Cited by 22 publications

References 43 publications

Gold nanoparticles assisted characterization of amine functionalized polystyrene multiwell plate and glass slide surfaces

Gold nanoparticles assisted characterization of amine functionalized polystyrene multiwell plate and glass slide surfaces

Scanometric Detection of Tomato Leaf Curl New Delhi Viral DNA Using Mono- and Bifunctional AuNP-Conjugated Oligonucleotide Probes

Fabrication of CdTe@SiO2 nanoprobes for sensitive electrogenerated chemiluminescence detection of DNA damage

Contact Info

Product

Resources

About