Visualisation of
                H2O2 penetration through skin
            indicates importance to develop pathway-specific epidermal sensing

Jankovskaja, Skaidre; Labrousse, Anaïs; Prévaud, Léa; Holmqvist, Bo; Brinte, Anders; Engblom, Johan; Rezeli, Melinda; Markó-Varga, György; Ruzgas, Tautgirdas

doi:10.1007/s00604-020-04633-9

Cited by 10 publications

(14 citation statements)

References 53 publications

(70 reference statements)

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“…This strategy is very beneficial to study how these nanosystems interact reversibly with skin and how they change the skin resistance for a short time period without affecting skin integrity [ 32 , 119 ]. The integrity of the skin in terms of skin impedance have been measured by four electrode assembly [ 119 , 172 ].…”

Section: Miscellaneousmentioning

confidence: 99%

Challenges in the Physical Characterization of Lipid Nanoparticles

et al. 2021

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Nano-sized drug transporters have become an efficient approach with considerable commercial values. Nanomedicine is not only limited to drug delivery by means of different administration routes, such as intravenous, oral, transdermal, nasal, pulmonary, and more, but also has applications in a multitude of areas, such as a vaccine, antibacterial, diagnostics and imaging, and gene delivery. This review will focus on lipid nanosystems with a wide range of applications, taking into consideration their composition, properties, and physical parameters. However, designing suitable protocol for the physical evaluation of nanoparticles is still conflicting. The main obstacle is concerning the sensitivity, reproducibility, and reliability of the adopted methodology. Some important techniques are compared and discussed in this report. Particularly, a comparison between different techniques involved in (a) the morphologic characterization, such as Cryo-TEM, SEM, and X-ray; (b) the size measurement, such as dynamic light scattering, sedimentation field flow fractionation, and optical microscopy; and (c) surface properties, namely zeta potential measurement, is described. In addition, an amperometric tool in order to investigate antioxidant activity and the response of nanomaterials towards the skin membrane has been presented.

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Section: Miscellaneousmentioning

confidence: 99%

Challenges in the Physical Characterization of Lipid Nanoparticles

et al. 2021

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“…This behavior in the case of SLN and ETHO could be attributed to an increased skin resistance in the presence of the nanoparticulate systems, as demonstrated by impedance measurements ( Figure 6 ). It is known that an increased resistance of skin usually slows down the permeation of hydrophilic solutes such as H 2 O 2 [ 55 ]. In order to better understand the amperometric response of SLN-CA, which is more intense with respect to ETHO-CA, an enzyme-modified oxygen electrode model has been employed.…”

Section: Resultsmentioning

confidence: 99%

The Potential of Caffeic Acid Lipid Nanoparticulate Systems for Skin Application: In Vitro Assays to Assess Delivery and Antioxidant Effect

et al. 2021

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The object of this study is a comparison between solid lipid nanoparticles and ethosomes for caffeic acid delivery through the skin. Caffeic acid is a potent antioxidant molecule whose cutaneous administration is hampered by its low solubility and scarce stability. In order to improve its therapeutic potential, caffeic acid has been encapsulated within solid lipid nanoparticles and ethosomes. The effect of lipid matrix has been evaluated on the morphology and size distribution of solid lipid nanoparticles and ethosomes loaded with caffeic acid. Particularly, morphology has been investigated by cryogenic transmission electron microscopy and small angle X-ray scattering, while mean diameters have been evaluated by photon correlation spectroscopy. The antioxidant power has been evaluated by the 2,2-diphenyl-1-picrylhydrazyl methodology. The influence of the type of nanoparticulate system on caffeic acid diffusion has been evaluated by Franz cells associated to the nylon membrane, while to evaluate caffeic acid permeation through the skin, an amperometric study has been conducted, which was based on a porcine skin-covered oxygen electrode. This apparatus allows measuring the O2 concentration changes in the membrane induced by polyphenols and H2O2 reaction in the skin. The antioxidative reactions in the skin induced by caffeic acid administered by solid lipid nanoparticles or ethosomes have been evaluated. Franz cell results indicated that caffeic acid diffusion from ethosomes was 18-fold slower with respect to solid lipid nanoparticles. The amperometric method evidenced the transdermal delivery effect of ethosome, indicating an intense antioxidant activity of caffeic acid and a very low response in the case of SLN. Finally, an irritation patch test conducted on 20 human volunteers demonstrated that both ethosomes and solid lipid nanoparticles can be safely applied on the skin.

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“…This means that most of the used sampling approaches reduced skin resistance variability, which might be beneficial in reducing variability of collected analytes due to more equalized fluxes across the skin. 27 …”

Section: Resultsmentioning

confidence: 99%

Non-Invasive, Topical Sampling of Potential, Low-Molecular Weight, Skin Cancer Biomarkers: A Study on Healthy Volunteers

et al. 2022

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Monitoring of low-molecular weight cancer biomarkers, such as tryptophan (Trp) and its derivative kynurenine (Kyn), might be advantageous to non-invasive skin cancer detection. Thus, we assessed several approaches of topical sampling of Trp and Kyn, in relation to phenylalanine (Phe) and tyrosine (Tyr), on the volar forearm of six healthy volunteers. The sampling was performed with three hydrogels (made of agarose or/and chitosan), hydrated starch films, cotton swabs, and tape stripping. The biomarkers were successfully sampled by all approaches, but the amount of collected Kyn was low, 20 ± 10 pmol/cm 2 . Kyn quantification was below LOQ, and thus, it was detected only in 20% of topical samples. To mitigate variability problems of absolute amounts of sampled amino acids, Tyr/Trp, Phe/Trp, and Phe/Tyr ratios were assessed, proving reduced inter-individual variation from 79 to 45% and intra-individual variation from 42 to 21%. Strong positive correlation was found between Phe and Trp, pointing to the Phe/Trp ratio (being in the 1.0–2.0 range, at 95% confidence) being least dependent on sampling materials, approaches, and sweating. This study leads to conclusion that due to the difficulty in quantifying less abundant Kyn, and thus the Trp/Kyn ratio, the Phe/Trp ratio might be a possible, alternative biomarker for detecting skin cancers.

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Visualisation of H2O2 penetration through skin indicates importance to develop pathway-specific epidermal sensing

Cited by 10 publications

References 53 publications

Challenges in the Physical Characterization of Lipid Nanoparticles

Challenges in the Physical Characterization of Lipid Nanoparticles

The Potential of Caffeic Acid Lipid Nanoparticulate Systems for Skin Application: In Vitro Assays to Assess Delivery and Antioxidant Effect

Non-Invasive, Topical Sampling of Potential, Low-Molecular Weight, Skin Cancer Biomarkers: A Study on Healthy Volunteers

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