Visualization of 3D Organoids Through the Latest Advancements in Microscopy

Kumar, Manoj; Rauthan, Riya; Chakraborty, Debojyoti; Arumugam, Senthil

doi:10.1007/978-1-0716-2720-4_3

Cited by 2 publications

(1 citation statement)

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“…Second, organoids comprise various biomolecules such as proteins, lipids, water, and minerals with wide-ranging refractive indices, resulting in substantial light scattering and absorption. Owing to these limitations, most cerebral organoid imaging studies rely on imaging sliced, very thin sections or superficial parts of cerebral organoids ( Tuchin et al, 1997 ; Yu et al, 2011 ; Feuchtinger et al, 2016 ; Grist et al, 2016 ; Seo et al, 2016 ; Kumar et al, 2023 ).…”

Section: Introductionmentioning

confidence: 99%

Applications of multiphoton microscopy in imaging cerebral and retinal organoids

Lacin,

Yildirim

2024

Front. Neurosci.

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Cerebral organoids, self-organizing structures with increased cellular diversity and longevity, have addressed shortcomings in mimicking human brain complexity and architecture. However, imaging intact organoids poses challenges due to size, cellular density, and light-scattering properties. Traditional one-photon microscopy faces limitations in resolution and contrast, especially for deep regions. Here, we first discuss the fundamentals of multiphoton microscopy (MPM) as a promising alternative, leveraging non-linear fluorophore excitation and longer wavelengths for improved imaging of live cerebral organoids. Then, we review recent applications of MPM in studying morphogenesis and differentiation, emphasizing its potential for overcoming limitations associated with other imaging techniques. Furthermore, our paper underscores the crucial role of cerebral organoids in providing insights into human-specific neurodevelopmental processes and neurological disorders, addressing the scarcity of human brain tissue for translational neuroscience. Ultimately, we envision using multimodal multiphoton microscopy for longitudinal imaging of intact cerebral organoids, propelling advancements in our understanding of neurodevelopment and related disorders.

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