Visualization of Experimental Glioma C6 by MRI with Magnetic Nanoparticles Conjugated with Monoclonal Antibodies to Vascular Endothelial Growth Factor

Abstract

We developed a method for obtaining iron oxide nanoparticles and their conjugation with monoclonal antibodies to vascular endothelial growth factor. The resultant vector nanoparticles were low-toxic and the antibodies retained their immunochemical activity after conjugation. The study was carried out on rats with intracranial glioma C6 on day 14 after its implantation. The intravenously injected nanoparticles visualized the brain tumor in contrast to nanoparticles conjugated with nonspecific immunoglobulins that did not accumulate in the tumor.

Precise Delivery Into Chronic Spinal Cord Injury Syringomyelic Cysts with Magnetic Nanoparticles MRI Visualization

“…Intra-cyst delivery was performed on the 7 rats of Group 2 with the help of the microinjection unit and Razel Syringe Pump (Razel scientific) system. The nanoparticles were designed as previously reported [ 17 ]. Using a 25-μl Nanofil syringe with a 33-gauge needle, 8 μl of nanoparticles were delivered into the syringomyelic cyst at 4 μl/min.…”

Vegfrs in Neoplastic Angiogenesis and Prospects for Therapy Оf Brain Tumors

“…Поэтому в по-следнее время изучают векторные контейнерные системы на основе антител к опухоль-ассоциированным белкам, таким как Her-2 [113], EGFR [114], GFAP и Cx-43 [102], VEGF [115] и VEGFR2 [116]. Векторизация контейнеров моноклональными антителами позволяет получить си-стему для адресной доставки лекарств.…”

Engineered protein–iron oxide hybrid biomaterial for MRI-traceable drug encapsulation

“…15 Furthermore, these constructs are typically comprised of protein conjugated to USPIOs that have either been purchased commercially 16 or previously synthesized under harsh conditions. 17,18 By contrast, biomineralization exploits organic biomolecules or biomimetics to serve as templates for controlled crystal formation under mild, even ambient, conditions. 19 In the case of iron oxide, magnetotactic bacteria such as Magnetospirillum magneticum 20 biomineralize uniform magnetite (Fe 3 O 4 ) nanoparticles within magnetosome organelles.…”