Visualization of gene expression of prolactin-receptor (PRL-R) by in situ hybridization in reproductive organs of Typhlonectes compressicauda, a gymnophionan amphibian

Abstract

The expression of the long form of prolactin receptor (PRL-R) mRNAs was studied in reproductive organs from both male and female Typhlonectes compressicauda, an amphibian, by quantitative in situ hybridization. These PRL-R mRNAs were expressed in all the organs studied. In ovarian tissue, mRNAs coding for the long form of PRL-R were strongly expressed in ovaries with compact and vascularized corpora lutea, i.e., during the middle and the end of pregnancy. During the other periods of cycle, sexual rest, vitellogenesis and beginning of pregnancy, expression of these mRNAs was lower than in other periods. In the testis, mRNAs coding for the long form of PRL-R were expressed in germ cells as well as in Leydig and Sertoli cells. In müllerian glands, important variations of expression in these mRNAs have been observed with a large increase during the breeding period and a decrease during sexual rest. Variations of gene expression of the long form of PRL-R for all tissues studied are correlated to synthesis of PRL in the pituitary in both male and female Typhlonectes compressicauda.

Identification of bovine prolactin in seminal fluid, and expression and localization of the prolactin receptor and prolactin-inducible protein in the testis and epididymis of bulls exposed to ergot alkaloids

“…Furthermore, PRLR was expressed in the spermatogonia and differentiating germ cells, which is consistent with previous reports [5,9,39] and could argue for some type of PRL effect on spermatogenesis. Prolactin receptor was expressed in the interstitial space in which Leydig cells are present.…”

Different expression of LHR, PRLR, GH and IGF1 during testicular development of yak

“…As expected, we found that PRLR was expressed in the yak kidney, liver, testicular, lung, skeletal muscle, heart and spleen and was consistent with observations reported for other species (Guillaumot et al., 1996; Lincoln et al., 2001; Pratt et al., 2015). In addition, PRLR was expressed in the Leydig cells and spermatogonia, which was consistent with the previous reports (Exbrayat & Morel, 2003; Hondo et al., 1995) and indicated that certain types of PRL affected spermatogenesis. Our results indicated that PRLR promoted spermatogenesis by effectively proliferation of Leydig cells during the development of yak testes.…”

Detection of the Hormone Receptors Controlling the Female Genital Tract throughout the Sexual Cycle of an Oviparous Caecilian Amphibian, Boulengerula taitana

“…In T. compressicauda, volumetric variations and number of lactotropic cells vary considerably throughout the cycle. An increase of PRL-encoding mRNAs was observed from the beginning to the end of pregnancy, whereas the ratio of PRL mRNAs remain constant and low during the other periods of the cycle [43,63]. These data suggest that PRL is a crucial endocrine factor that controls the development of the genital tract during the reproductive period of both viviparous and oviparous caecilian species.…”