2009
DOI: 10.1508/cytologia.74.449
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Visualization of Mitochondrial and Apicoplast Nucleoids in the Human Malaria Parasite Plasmodium falciparum by SYBR Green I and PicoGreen Staining

Abstract: Summary Organellar DNA in mitochondria and plastids are organized with proteins into a compact structure known as the nucleoid. As the nucleoid is supposed to be the unit of inheritance for the organellar genome, it is important to understand its cytological behavior. Like plants, malaria parasites carry two organelles, the mitochondrion and the apicoplast-a non-photosynthetic plastid. However, probably because of the small size of the genome in each, visualizing the nucleoid in the Plasmodium organelles by re… Show more

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Cited by 9 publications
(4 citation statements)
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“…One of the main proposed roles of mitochondrial fusion is to control the quality of mitochondrial DNA among individual mitochondria in the cell, which may be damaged by reactive oxygen species generated during respiration [3]. Given that parasites from Apicomplexa and Kinetoplastida groups contain just a single mitochondrion with one mitochondrial nucleoid [51,93,94], this role of mitochondrial fusion may not be necessary. Conversely, MROs such as hydrogenosomes or mitosomes are usually present in large numbers in protists living in anoxic environments.…”
Section: Ancestral or Acquired Similarities?mentioning
confidence: 99%
“…One of the main proposed roles of mitochondrial fusion is to control the quality of mitochondrial DNA among individual mitochondria in the cell, which may be damaged by reactive oxygen species generated during respiration [3]. Given that parasites from Apicomplexa and Kinetoplastida groups contain just a single mitochondrion with one mitochondrial nucleoid [51,93,94], this role of mitochondrial fusion may not be necessary. Conversely, MROs such as hydrogenosomes or mitosomes are usually present in large numbers in protists living in anoxic environments.…”
Section: Ancestral or Acquired Similarities?mentioning
confidence: 99%
“…1b). SYBR green I binds to double-stranded DNA and is one of the most sensitive fluorescent chemical dyes for the visualization of nuclei or organelle nucleoids in living cells (Nishimura et al 1998, Arimura et al 2004, Maeda-Sano et al 2009. Unlike with SYBR Green I staining, SYTOX Orange did not label the nuclei specifically but it stained other SCN organs, including the stylet and esophageal lumen (Fig.…”
Section: Fluorescent Labeling Of Scns With Sybr Green I Sytox Orangementioning
confidence: 99%
“…1, 2b). Because the suitability of FM4-64 and SYBR green I in live imaging is well established (Betz et al 1992, Nishimura et al 1998, Arimura et al 2004, Maeda-Sano et al 2009, both dyes were used in the experiments described below.…”
Section: Fluorescent Labeling Of Scns With Sybr Green I Sytox Orangementioning
confidence: 99%
“…Immunolabeling using an anti-DNA antibody [78] or fluorescent in situ hybridization (FISH) [79] is commonly selected in fixed cells. In addition, several fluorescent DNA intercalators, such as 4 0 ,6 0 -diamidino-2phenylindole (DAPI) [80], SYTO13 [81], ethidium bromide [78,82], SYBR Green I [83,84], PicoGreen [85], and ditercalinium chloride [86], have been shown to label mtDNA nucleoids in living cultured cells or organisms. These fluorescent dyes are useful in a multistaining procedure because they have different excitation and emission spectrums.…”
Section: Visualization Of Mitochondrial Genome/nucleoids In Germ Cellsmentioning
confidence: 99%