Visualization of odor-induced neuronal activity by immediate early gene expression

Bepari, Asim Kumar; Watanabe, Keisuke; Yamaguchi, Masahiro; Tamamaki, Nobuaki; Takebayashi, Hirohide

doi:10.1186/1471-2202-13-140

Cited by 53 publications

(55 citation statements)

References 60 publications

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“…Scale bar: 50 μm in P for E-P; 10 μm in insets in H,L,P. Nakashima et al, 2003;Akiba et al, 2009;Kress and Wullimann, 2012;Bepari et al, 2012). We show that ZIF268 induction is severely impaired in the GL of COUP-TFI mutant mice, both in basal condition and following acute odour stimulation, supporting a role for COUP-TFI in the control of OB interneuron responsiveness.…”

Section: Discussionmentioning

confidence: 56%

COUP-TFI controls activity-dependent tyrosine hydroxylase expression in adult dopaminergic olfactory bulb interneurons

et al. 2013

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COUP-TFI is an orphan nuclear receptor acting as a strong transcriptional regulator in different aspects of forebrain embryonic development. In this study, we investigated COUP-TFI expression and function in the mouse olfactory bulb (OB), a highly plastic telencephalic region in which continuous integration of newly generated inhibitory interneurons occurs throughout life. OB interneurons belong to different populations that originate from distinct progenitor lineages. Here, we show that COUP-TFI is highly expressed in tyrosine hydroxylase (TH)-positive dopaminergic interneurons in the adult OB glomerular layer (GL). We found that odour deprivation, which is known to downregulate TH expression in the OB, also downregulates COUP-TFI in dopaminergic cells, indicating a possible correlation between TH-and COUP-TFI-activitydependent action. Moreover, we demonstrate that conditional inactivation of COUP-TFI in the EMX1 lineage results in a significant reduction of both TH and ZIF268 expression in the GL. Finally, lentiviral vector-mediated COUP-TFI deletion in adult-generated interneurons confirmed that COUP-TFI acts cell-autonomously in the control of TH and ZIF268 expression. These data indicate that COUP-TFI regulates TH expression in OB cells through an activitydependent mechanism involving ZIF268 induction and strongly argue for a maintenance rather than establishment function of COUP-TFI in dopaminergic commitment. Our study reveals a previously unknown role for COUP-TFI in the adult brain as a key regulator in the control of sensory-dependent plasticity in olfactory dopaminergic neurons.

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Section: Discussionmentioning

confidence: 56%

COUP-TFI controls activity-dependent tyrosine hydroxylase expression in adult dopaminergic olfactory bulb interneurons

et al. 2013

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“…A few O-MACS and c-fos double-positive glomeruli were found close to the border of O-MACS + domain of ΔD(cng) mice. These glomeruli might be innervated by olfactory neurons in which neural activity is not dependent on CNGA2 function (41,42). In ΔD(dta) mice, O-MACS + dorsal glomeruli were eliminated and the remaining O-MACS − domain was activated by SBT ( Fig.…”

Section: Altered Aversive Behaviors Induced By An Alarm Pheromone In δDmentioning

confidence: 98%

Genetic dissection of pheromone processing reveals main olfactory system-mediated social behaviors in mice

Matsuo¹,

Hattori

Asaba

et al. 2015

Proc. Natl. Acad. Sci. U.S.A.

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Most mammals have two major olfactory subsystems: the main olfactory system (MOS) and vomeronasal system (VNS). It is now widely accepted that the range of pheromones that control social behaviors are processed by both the VNS and the MOS. However, the functional contributions of each subsystem in social behavior remain unclear. To genetically dissociate the MOS and VNS functions, we established two conditional knockout mouse lines that led to either loss-of-function in the entire MOS or in the dorsal MOS. Mice with whole-MOS loss-of-function displayed severe defects in active sniffing and poor survival through the neonatal period. In contrast, when loss-of-function was confined to the dorsal MOB, sniffing behavior, pheromone recognition, and VNS activity were maintained. However, defects in a wide spectrum of social behaviors were observed: attraction to female urine and the accompanying ultrasonic vocalizations, chemoinvestigatory preference, aggression, maternal behaviors, and risk-assessment behaviors in response to an alarm pheromone. Functional dissociation of pheromone detection and pheromonal induction of behaviors showed the anterior olfactory nucleus (AON)-regulated social behaviors downstream from the MOS. Lesion analysis and neural activation mapping showed pheromonal activation in multiple amygdaloid and hypothalamic nuclei, important regions for the expression of social behavior, was dependent on MOS and AON functions. Identification of the MOS-AON-mediated pheromone pathway may provide insights into pheromone signaling in animals that do not possess a functional VNS, including humans.social behavior | pheromone processing | main olfactory system | vomeronasal system M ost mammals have two major olfactory subsystems-the main olfactory system (MOS) and vomeronasal system (VNS). The MOS comprises the main olfactory epithelium (MOE), in which olfactory sensory neurons detect odorants, and their projection target, the main olfactory bulb (MOB) (Fig. S1A). Although the MOS is thought to detect volatile odorants and the VNS is thought to be important for the detection of nonvolatile pheromones, evidence shows that the MOS is also involved in pheromone detection (1-8). Surgical blocking of odorant access to the MOE, but not surgical ablation of the vomeronasal epithelium (VNE), eliminates preference to odors from the opposite sex in ferrets (9, 10). In mice, chemical ablation of the MOE impairs male and female sexual behaviors (11,12). In these experiments in which the MOE was ablated, the function of the VNS is not directly disrupted, because the VNS is activated by direct application of urine to the nostril. Thus, these results indicate that the MOS also contributes to pheromone processing and related behaviors.Nonconditional disruption of genes encoding signal transduction proteins that are required for activation of olfactory neurons, such as cyclic nucleotide-gated channel (Cnga2) or adenylyl cyclase 3, impairs several social behaviors (11,(13)(14)(15). However, complete loss of MOS function causes a...

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“…cFos is an IEG that has shown up-regulation due to a multitude of stimuli 22 , examples including: activation in olfactory bulbs due to scent 23 , expression in striatum due to caffeine intake 26 , and activation in auditory cortex due to auditory cues 27 . cFos is able to auto-regulate itself, by a negative feedback loop 15 .…”

Section: Cfosmentioning

confidence: 99%

“…cFos is also an ideal marker for neuronal activation, in which its expression increases in brain regions when exposed to associated stimuli 23 . Also, cFos is unique from other IEG's in that its basal levels are relatively low, there is a broad range of mRNA levels, and both mRNA and protein have a short half-life 24 .…”

Section: Cfosmentioning

confidence: 99%

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Effects of Transcranial Direct Current Stimulation on Expression of Immediate Early Genes (IEG's)

Wagner

2015

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Visualization of odor-induced neuronal activity by immediate early gene expression

Cited by 53 publications

References 60 publications

COUP-TFI controls activity-dependent tyrosine hydroxylase expression in adult dopaminergic olfactory bulb interneurons

COUP-TFI controls activity-dependent tyrosine hydroxylase expression in adult dopaminergic olfactory bulb interneurons

Genetic dissection of pheromone processing reveals main olfactory system-mediated social behaviors in mice

Effects of Transcranial Direct Current Stimulation on Expression of Immediate Early Genes (IEG's)

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