Visualization of oxygen distribution on leaf surfaces using VisiSens oxygen planar optode system

Abstract

Abstract.Oxygen is a key factor in aerobic reactions and most biological activities. Visualization of oxygen distribution of a chemical process or biological system has been a very challenging object despite of its significance and potential impact. To monitor and visualize the spatial distribution of oxygen concentration, various techniques such as electro-chemical probe, polarographic electrode, LIF(laser-induced fluorescence) and so on have been introduced. Oxygen planar optode which utilizes the oxygen quenching of fluorescence light is one of the currently available methods for time-resolved visualization of oxygen distribution on a planar surface. In this study, we utilized VisiSens oxygen planar optode system to visualize the spatial distribution of oxygen concentration on leaves of Korean azalea. As a result, temporal variation of oxygen concentration distribution caused by respiratory activity of the leaf could be quantitatively monitored. . 1990년대에는 산소 광소멸(oxygen quenching)을 일으 키는 발광 물질(luminophore)을 이용한 산소 평면광 센서 가 개발되었다 (3,4) . PtOEP(platinum octaethylporphyrin)와 같은 발광 물질은 접촉한 환경의 산소 농도에 따라 발광 강도(intensity)가 다르게 나타나며, 이를 촬영한 영상을 분 석하면 산소의 농도분포를 측정할 수 있다. 주로 박막 형 태의 평면에 적용 가능하다는 문제점은 있지만, 탐침 (probe)을 측정단면을 스캔시키는 전기화학적(electro-

A novel approach to investigate hypoxic microenvironment during rice colonization by Magnaporthe oryzae

“…In previous studies (Littlejohn et al ., ; Tschiersch et al ., ; Hwang et al ., ), a perfluorodecalin (PFD) solution was placed between a plant sample and the sensor foil, instead of water. As the PFD solution has a much higher capacity for dissolving oxygen than water and less physiological impact on the mounted plant sample, it is expected to precisely detect the O 2 concentration distribution of the leaf surface.…”

“…We calibrated the sensor foil using an oxygen scavenging agent (0% oxygen saturation; Cal0) and ambient air (100% oxygen saturation; Cal100) to make a standard for measuring fluorescence before using the sensor (Fig. B) (Tschiersch et al ., ; Hwang et al ., ). The Cal0 condition was created by placing a drop of 1 M Na 2 SO 3 solution on the side of the sensor foil for 10 min.…”