Visualizing odor representation in the brain: a review of imaging techniques for the mapping of sensory activity in the olfactory glomeruli

Pain, Frédéric; L'Heureux, Barbara; Gurden, Hirac

doi:10.1007/s00018-011-0708-4

Cited by 118 publications

(21 citation statements)

References 133 publications

(238 reference statements)

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“…2(c), 2(d)). This result was also consistent with the existing reports of OISI maps with the medial part of OB activated by propionic acid while m-cresol activated the lateral dorsal region [5,9]. In order to confirm the validity of the functional signal, as a control experiment, OCT scans were performed without odor stimulation at the same position across the blue line of Figs.…”

Section: Conventional Optical Intrinsic Signal Imaging (Oisi)supporting

confidence: 91%

“…OISI that has a lateral resolution of a few micrometers resolution does not need any exogenous treatment and can cover over large cortical areas [1][2][3][4][5][6][7][8][9][10]. However, in OISI due to imaging by a CCD system, the OISI map is visualized as integration of detected light from different depths and hence there is no spatial resolution for depth direction (z-axis).…”

Section: Introductionmentioning

confidence: 99%

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Functional optical coherence tomography of rat olfactory bulb with periodic odor stimulation

Watanabe¹,

Rajagopalan²,

Nakamichi³

et al. 2016

Biomed. Opt. Express

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Abstract:In rodent olfactory bulb (OB), optical intrinsic signal imaging (OISI) is commonly used to investigate functional maps to odorant stimulations. However, in such studies, the spatial resolution in depth direction (z-axis) is lost because of the integration of light from different depths. To solve this problem, we propose functional optical coherence tomography (fOCT) with periodic stimulation and continuous recording. In fOCT experiments of in vivo rat OB, propionic acid and m-cresol were used as odor stimulus presentations. Such a periodic stimulation enabled us to detect the specific odorresponses from highly scattering brain tissue. Swept source OCT operating at a wavelength of 1334 nm and a frequency of 20 kHz, was employed with theoretical depth and lateral resolutions of 6.7 μm and 15.4 μm, respectively. We succeeded in visualizing 2D cross sectional fOCT map across the neural layer structure of OCT in vivo. The detected fOCT signals corresponded to a few glomeruli of the medial and lateral parts of dorsal OB. We also obtained 3D fOCT maps, which upon integration across z-axis agreed well with OISI results. We expect such an approach to open a window for investigating and possibly addressing toward inter/intra-layer connections at high resolutions in the future. 181-192 (1997). 33. Y. Nakamichi, V. A. Kalatsky, H. Watanabe, U. M. Rajagopalan, and M. Tanifuji, "3D structure of the orientation column in cat primary cortex revealed by functional optical coherence tomography," Abstr. Soc. Neurosci. 270. 01 (2011), http://www.abstractsonline.com/Plan/ViewAbstract.aspx?sKey=d023089b-a64b-4aa9-b3cb-6129bde54e45&cKey=09b7566c-c153-4692-8113-1a04e1af0b1d&mKey=%7b8334BE29-8911-4991-8C31- 3067-3086 (2012).

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Section: Conventional Optical Intrinsic Signal Imaging (Oisi)supporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Functional optical coherence tomography of rat olfactory bulb with periodic odor stimulation

Watanabe¹,

Rajagopalan²,

Nakamichi³

et al. 2016

Biomed. Opt. Express

View full text Add to dashboard Cite

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“…Although the intrinsic signal mostly measures indirect effects such as local changes in the blood stream in the vicinity of actively firing neurons, results obtained are rather similar to those of calcium imaging performed specifically in the presynaptic axon terminals of olfactory receptor neurons [44,47].…”

Section: Imaging Odor Responses In the Olfactory Bulbsupporting

confidence: 54%

The peripheral olfactory system of vertebrates: molecular, structural and functional basics of the sense of smell

Manzini

Korsching

2011

E-Neuroforum

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The sense of smell provides people and animals with an abundance of information about their environment, helping them to navigate, detect potential threats, control food intake, choose sexual partners and significantly influence intraspecies social behavior. The perception of odors begins with the binding of odor molecules to specialized olfactory receptor proteins, which nearly all belong to the superfamily of G protein-coupled receptors. Altogether, five different olfactory receptor gene families have been described to date, among them the largest gene family in the genome with over 1000 genes in rodents. The signal transduction cascade coupled to the receptors has already been well characterized for this family. Three different classes of receptor neurons-ciliated, microvillous and crypt receptor neurons-can be distinguished by their anatomical and molecular characteristics. Generally, an individual receptor neuron expresses only a single olfactory receptor gene, and olfactory receptor neurons that express the same receptor converge into a common target structure, a glomerulus, which generates a receptotopic map in the first olfactory brain region, the olfactory bulb. This review article provides a general overview of the peripheral detection of odorants on the one hand, while on the other it focuses on recent advances in the field, including new findings on the peripheral modulation of olfactory signals.

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“…Wide-field bioluminescence has become a standard for fast screening of small animal models of cancers in preclinical oncology. 1 In vivo optical imaging at the cellular level, such as two-photon fluorescence imaging, second harmonic generation imaging, or wide-field imaging techniques at the structure or organ level (intrinsic optical imaging, calcium or sensitive dye imaging), have become routine tools in labs, 2 especially in the neuroscience field. These techniques produce new data, unraveling structural and functional mechanisms, and have been adopted quickly as reference tools.…”

mentioning

confidence: 99%

Experimental and analytical comparative study of optical coefficient of fresh and frozen rat tissues

Mesradi

Genoux

Cuplov³

et al. 2013

J. Biomed. Opt

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Abstract. Optical properties of fresh and frozen tissues of rat heart, kidney, brain, liver, and muscle were measured in the 450-to 700-nm range. The total reflectance and transmittance were measured using a well-calibrated integral sphere set-up. Absorption coefficient μ a and reduced scattering coefficient μ 0 s were derived from the experimental measurements using the inverse adding doubling technique. The influence of cryogenic processing on optical properties was studied. Interindividual and intraindividual variations were assessed. These new data aim at filling the lack of validated optical properties in the visible range especially in the blue-green region of particular interest for fluorescence and optogenetics preclinical studies. Furthermore, we provide a unique comparison of the optical properties of different organs obtained using the same measurement set-up for fresh and frozen tissues as well as an estimate of the intraindividual and interindividual variability.

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Visualizing odor representation in the brain: a review of imaging techniques for the mapping of sensory activity in the olfactory glomeruli

Cited by 118 publications

References 133 publications

Functional optical coherence tomography of rat olfactory bulb with periodic odor stimulation

Functional optical coherence tomography of rat olfactory bulb with periodic odor stimulation

The peripheral olfactory system of vertebrates: molecular, structural and functional basics of the sense of smell

Experimental and analytical comparative study of optical coefficient of fresh and frozen rat tissues

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