Vital Staining of Trichomonas Vaginalis With FluoresceiN

Coutts, Waldemar E.; Silva-Inzunza, Edna

doi:10.1136/sti.30.1.43

“…Other stains such as safranin, methylene blue, and malachite green, which do not stain trophozoites, can act as counterstains [ 76 , 77 , 78 ]. Fluorescein can also be used to observe wet mount slides under an ultraviolet light microscope [ 79 ]. Thus, acridine orange for fluorescence-based detection of T. vaginalis has also been suggested by other authors as it exhibits a greater sensitivity than Giemsa staining but requires UV fluorescent light microscopy [ 47 , 80 ].…”

Section: Classical Diagnostic Procedures: Microscopymentioning

confidence: 99%

The Past, Present, and Future in the Diagnosis of a Neglected Sexually Transmitted Infection: Trichomoniasis

Ibáñez-Escribano,

Nogal-Ruiz

2024

Pathogens

4

0

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More than one million curable sexually transmitted infections occur every day. Trichomonas vaginalis is one of the main infections responsible for these epidemiological data; however, the diagnosis of this protozoan is still mainly based on microscopic and culture identification. The commercialization of immunological tests and the development of molecular techniques have improved the sensitivity of classical methods. Nevertheless, the fact that trichomoniasis is a neglected parasitic infection hinders the development of novel techniques and their implementation in routine diagnosis. This review article shows the different methods developed to identify T. vaginalis in population and the difficulties in diagnosing male and asymptomatic patients. The importance of including this parasite in routine gynecological screening, especially in pregnant women, and the importance of considering T. vaginalis as an indicator of high-risk sexual behavior are also discussed.

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“…Other stains such as safranin, methylene blue and malachite green, which do not stain trophozoites, can act as counterstains [76][77][78]. Fluorescein can also be used to observe wet mount slides under an ultraviolet light microscope [79]. Thus, acridine orange for fluorescence-based detection of T. vaginalis has also been suggested by other authors as exhibits a greater sensitivity than Giemsa staining but requires UV fluorescent light microscopy [47,80].…”

Section: Staining Techniquesmentioning

confidence: 99%

The Past, Present and Future in the Diagnosis of a Neglected Sexually Transmitted Infection: Trichomoniasis

Ibáñez-Escribano,

Nogal-Ruiz

2024

Preprint

2

0

View full text Add to dashboard Cite

More than one million curable sexually transmitted infections occur every day. Trichomonas vaginalis is one of the main responsible for these epidemiological data, however, the diagnosis of this protozoan is still mainly based on microscopic and culture identification. The commerciali-zation of immunological tests and the development of molecular techniques have improved the sensitivity of classical methods. Nevertheless, the fact that trichomoniasis is a neglected parasitic infection, hinders the development of novel techniques and their implementation in routine di-agnosis. This review article shows the different methods developed to identify T. vaginalis in population and the difficulties to diagnose male and asymptomatic patients. The importance of including this parasite in routine gynecological screening, especially in pregnant women, and the importance of considering T. vaginalis as an indicator of high-risk sexual behavior is also discussed.

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“…However, these staining methods has not been shown convincingly to improve the detection rate of trichomonads in secretions and is not recommended for the routine clinical laboratory. Vital staining of T. vaginalis can be accomplished by addition of fluorescein to the saline mount preparation, in dark field illumination these organisms appear emerald green [73].…”

Section: Staining Techniquesmentioning

confidence: 99%

The Past, Present and Future in the Diagnosis of a Neglected Sexually Transmitted Infection: Trichomoniasis

Ibáñez-Escribano,

Nogal-Ruiz

2023

Preprint

0

View full text Add to dashboard Cite

More than one million curable sexually transmitted infections occur every day. Trichomonas vaginalis is one of the main responsible for these epidemiological data, however, the diagnosis of this protozoan is still mainly based on microscopic and culture identification. The commerciali-zation of immunological tests and the development of molecular techniques have improved the sensitivity of classical methods. Nevertheless, the fact that trichomoniasis is a neglected parasitic infection, hinders the development of novel techniques and their implementation in routine di-agnosis. This review article shows the different methods developed to identify T. vaginalis in population and the difficulties to diagnose male and asymptomatic patients. The importance of including this parasite in routine gynecological screening, especially in pregnant women, and the importance of considering T. vaginalis as an indicator of high-risk sexual behavior is also discussed.

show abstract

Vital Staining of Trichomonas Vaginalis With FluoresceiN

Cited by 5 publications

References 0 publications

The Past, Present, and Future in the Diagnosis of a Neglected Sexually Transmitted Infection: Trichomoniasis

The Past, Present, and Future in the Diagnosis of a Neglected Sexually Transmitted Infection: Trichomoniasis

The Past, Present and Future in the Diagnosis of a Neglected Sexually Transmitted Infection: Trichomoniasis

The Past, Present and Future in the Diagnosis of a Neglected Sexually Transmitted Infection: Trichomoniasis

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