Vitamin A injection at birth improves muscle growth in lambs

Song, Pengkang; Chen, Xiaoyou; Zhao, Jianlong; Liu, Qiang; Li, Xinrui; Wang, Yu; Wang, Bo; Zhao, Junxing

doi:10.1016/j.aninu.2023.05.011

Cited by 6 publications

(8 citation statements)

References 38 publications

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“…Therefore, understanding the mechanisms of skeletal muscle development is urgently needed to improve meat production in sheep. In previous studies, we found that intramuscular injection of vitamin A promotes muscle development in lambs [ 17 ]. However, molecular regulation during skeletal muscle development is highly sophisticated and complex.…”

Section: Discussionmentioning

confidence: 99%

“…Studies have shown that RA promotes myogenesis in zebrafish [ 14 ] and Angus cattle [ 15 ], while inhibit differentiation of chick embryo limb myoblasts [ 16 ]. Our previous publication indicated that intramuscular injection of vitamin A increased final body weight and loin eye area of lambs, and RA promoted sheep primary myoblasts early differentiation in vitro [ 17 ]. In the current study, we continue to investigate the mechanism by which vitamin A affect sheep skeletal muscle growth.…”

Section: Introductionmentioning

confidence: 99%

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Vitamin a potentiates sheep myoblasts myogenic differentiation through BHLHE40-modulated ID3 expression

Song,

Zhao,

Zhang

et al. 2024

BMC Genomics

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Background Vitamin A and retinoic acid (RA, a metabolite of vitamin A), are inextricably involved to the development of skeletal muscle in animals. However, the mechanisms regulating skeletal muscle development by vitamin A remain poorly reported. The current study designed to investigate the underlying mechanism of vitamin A affecting myogenic differentiation of lamb myoblasts through transcriptome sequencing (RNA-Seq) and gene function validation experiments. It provides a theoretical basis for elucidating the regulation of vitamin A on skeletal muscle development as well as for improving the economic benefits of the mutton sheep industry. Results Newborn lambs were injected with 7,500 IU vitamin A, and longissimus dorsi (LD) muscle tissue was surgically sampled for RNA-Seq analysis and primary myoblasts isolation at 3 weeks of age. The results showed that a total of 14 down-regulated and 3 up-regulated genes, were identified between control and vitamin A groups. Among them, BHLHE40 expression was upregulated in vitamin A group lambs. Furthermore, BHLHE40 expression is significantly increased after initiation of differentiation in myoblasts, and RA addition during differentiation greatly promoted BHLHE40 mRNA expression. In vitro, RA inhibited myoblasts proliferation and promoted myoblasts myogenic differentiation through BHLHE40. Moreover, BHLHE40 was proved to inhibit the expression of the DNA binding inhibitor 3 (ID3), and meanwhile, ID3 could effectively promote myoblasts proliferation and inhibit myoblasts myogenic differentiation. Conclusions Taken together, our results suggested that vitamin A inhibited myoblasts proliferation and promoted myoblasts myogenic differentiation by inhibiting ID3 expression through BHLHE40.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Vitamin a potentiates sheep myoblasts myogenic differentiation through BHLHE40-modulated ID3 expression

Song,

Zhao,

Zhang

et al. 2024

BMC Genomics

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“…From weaning to harvest, the daily intake of concentrate was increased from 0.3 to 1.75 kg, and grass hay was increased from 0.25 to 0.5 kg per lamb. The ingredients and nutrient composition of the diet, which contains 13,000 IU VA / kg concentrate, and the growth performance of the lambs have been previously published ( 17 ). They were slaughtered until 8 months of age for blood, testis, and epididymis tissue collection.…”

Section: Methodsmentioning

confidence: 99%

Neonatal vitamin A supplementation improves sheep fertility potential

Li,

Song,

Zhao

et al. 2024

Front. Vet. Sci.

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This study aimed to explore the effects of neonatal vitamin A (VA) supplementation on testis development and spermatogenesis. A total of 32 newborn lambs were intramuscularly injected with corn oil (control group) or corn oil + 2500 IU/kg BW VA (VA group). They were slaughtered and sampled at 3 weeks and 8 months of age to analyze spermatogenesis, cell proliferation, hormone secretion, antioxidant status of the testis, and adult sheep sperm parameters. Compared with the control group, the expression of spermatogonial differentiation-related genes in VA group was up-regulated (P < 0.05). Testis weight, seminiferous tubule diameter, number of spermatogonium and spermatocyte, and sperm density increased significantly in VA group at 8 months of age (P < 0.05). Neonatal VA injection upregulated the expression of the cell proliferation marker PCNA and cell cycle-related genes in the testis (P < 0.05). VA increased the concentrations of testosterone (T), luteinizing hormone (LH), and follicle-stimulating hormone (FSH) in the serum and upregulated steroidogenesis-related genes in the testis (P < 0.05). The antioxidant levels in the VA group were maintained at high levels. The total antioxidant capacity (T-AOC), antioxidant enzyme content and antioxidant-related genes were increased in the testis (P < 0.05). Furthermore, neonatal VA injection activated retinoic acid (RA) signaling to maintain the blood-testosterone barrier (BTB) in the testis of 3-week-old sheep. AMP-activated protein kinase (AMPK) and protein kinase B (AKT) signaling were also modulated in the sheep testis (P < 0.05). Taken together, VA supplementation in newborn rams promotes testis development and spermatogenesis to improve fertility.

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“…Moreover, grass hay (peanut seedlings) was added to promote lamb growth during the finishing period. Nutrient composition of concentrate diet and grass hay have been reported in another manuscript [ 34 ]. For peanut seedlings, the AOAC method [ 35 ] was used to determine DM content, and the Van Soest method [ 36 ] was used to determine NDF and ADF contents.…”

Section: Methodsmentioning

confidence: 99%

“…Sheep primary myoblasts were isolated according to the method previously described [ 34 ]. When the cell fusion reached 100%, the differentiation medium containing RA and 2% horse serum was replaced.…”

Section: Methodsmentioning

confidence: 99%

Vitamin A regulates mitochondrial biogenesis and function through p38 MAPK-PGC-1α signaling pathway and alters the muscle fiber composition of sheep

Song,

Zhao,

et al. 2024

J Animal Sci Biotechnol

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Background Vitamin A (VA) and its metabolite, retinoic acid (RA), are of great interest for their wide range of physiological functions. However, the regulatory contribution of VA to mitochondrial and muscle fiber composition in sheep has not been reported. Method Lambs were injected with 0 (control) or 7,500 IU VA palmitate into the biceps femoris muscle on d 2 after birth. At the age of 3 and 32 weeks, longissimus dorsi (LD) muscle samples were obtained to explore the effect of VA on myofiber type composition. In vitro, we investigated the effects of RA on myofiber type composition and intrinsic mechanisms. Results The proportion of type I myofiber was greatly increased in VA-treated sheep in LD muscle at harvest. VA greatly promoted mitochondrial biogenesis and function in LD muscle of sheep. Further exploration revealed that VA elevated PGC-1α mRNA and protein contents, and enhanced the level of p38 MAPK phosphorylation in LD muscle of sheep. In addition, the number of type I myofibers with RA treatment was significantly increased, and type IIx myofibers was significantly decreased in primary myoblasts. Consistent with in vivo experiment, RA significantly improved mitochondrial biogenesis and function in primary myoblasts of sheep. We then used si-PGC-1α to inhibit PGC-1α expression and found that si-PGC-1α significantly abrogated RA-induced the formation of type I myofibers, mitochondrial biogenesis, MitoTracker staining intensity, UQCRC1 and ATP5A1 expression, SDH activity, and enhanced the level of type IIx muscle fibers. These data suggested that RA improved mitochondrial biogenesis and function by promoting PGC-1α expression, and increased type I myofibers. In order to prove that the effect of RA on the level of PGC-1α is caused by p38 MAPK signaling, we inhibited the p38 MAPK signaling using a p38 MAPK inhibitor, which significantly reduced RA-induced PGC-1α and MyHC I levels. Conclusion VA promoted PGC-1α expression through the p38 MAPK signaling pathway, improved mitochondrial biogenesis, and altered the composition of muscle fiber type. Graphical Abstract

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Vitamin A injection at birth improves muscle growth in lambs

Cited by 6 publications

References 38 publications

Vitamin a potentiates sheep myoblasts myogenic differentiation through BHLHE40-modulated ID3 expression

Vitamin a potentiates sheep myoblasts myogenic differentiation through BHLHE40-modulated ID3 expression

Neonatal vitamin A supplementation improves sheep fertility potential

Vitamin A regulates mitochondrial biogenesis and function through p38 MAPK-PGC-1α signaling pathway and alters the muscle fiber composition of sheep

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