VITAMIN B<sub>1</sub> IN THE NUTRITION OF EXCISED TOMATO ROOTS

White, Philip R.

doi:10.1104/pp.12.3.803

Cited by 46 publications

(9 citation statements)

References 2 publications

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“…Recent results in our laboratory suggest that A. oppositifolia photosynthetic roots do not have an obligate requirement for thiamine (]. Cuello and H. Flores, unpublished observations), a vitamin previously thought to be required by heterotrophic roots (White, 1937;Bonner and Bonner, 1948 folia, and COz enrichment was necessary for optimal growth (Table I). Although dark-grown cultures showed some residual growth in the presence of COz, these roots failed to grow in subsequent transfers.…”

Section: Resultsmentioning

confidence: 90%

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Green Roots: Photosynthesis and Photoautotrophy in an Underground Plant Organ

et al. 1993

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l h e potential for photosynthetic and photoautotrophic growth was studied in hairy root cultures of Asteraceae and Solanaceae species. Upon transfer to light, initially heterotrophic root cultures of Acmella oppositifolia and Dafura innoxia greened rapidly, differentiated chloroplasts, and developed light-dependent CO, fixation in the cortical cells. Photosynthetic potential was expressed in root cultures of all the Asteraceae genera examined (Acmella, Artemisia, Rudbeckia, Sfevia, and Tagetes). Hairy roots of A. oppositifolia and D. innoxia were further adapted to photoautotrophy by growing in the presence of light and added CO, (1-5%) and by direct or sequential transfers into media containing progressively lower sugar concentrations. l h e transition to photoautotrophy was accompanied by an increase in COz fixation and in the specific activity of 1,s-ri bulose-bisphosphate carboxylase/ oxygenase (Rubisco). During the adaptation of A. opposififolia roots to photoautotrophy, the ratio of Rubisco to phosphoenolpyruvate carboxylase increased significantly, approaching that found in the leaves. l h e levels and patterns of alkaloids and polyacetylenes produced by Solanaceae and Asteraceae hairy roots, respectively, were dramatically altered in photomixotrophic and photoautotrophic cultures. Photoautotrophic roots of A. opposififolia have been maintained in vitro for over 2 years.

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Section: Resultsmentioning

confidence: 90%

“…It is also well known that isolated root cultures are dependent on vitamins such as thiamine for continuous growth in vitro (White, 1937). The tap roots of weeds such as dandelions (Peterson, 1975) and many in vitro root cultures can regenerate green shoots, suggesting that the root cells remain totipotent.…”

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confidence: 99%

Green Roots: Photosynthesis and Photoautotrophy in an Underground Plant Organ

et al. 1993

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“…Thiamine is involved in cell biosynthesis and metabolism (Willims, 1995). It is particularly well known that the isolated root cultures are dependent on thiamine for continuous growth in vitro (White, 1937). On varying the concentration of minor salts, Fe-EDTA and vitamins it was observed that, normal concentrations of MS minor salts supported maximum growth and solasodine production ( Figure 4).…”

Section: Comparison Between Ms and B5 Medium In Terms Of Growth And Pmentioning

confidence: 97%

Manipulation of MS and B5 components for enhancement of growth and solasodine production in hairy root cultures of Solanum khasianum Clarke

Jacob

Malpathak

2005

Plant Cell Tiss Organ Cult

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Hairy root cultures of Solanum khasianum Clarke were studied for growth and solasodine production by manipulating the major and minor salts, Fe-EDTA and vitamins of MS and B5 medium. Tissue growth and solasodine production are strongly affected by the culture medium. The experiments demonstrated that in part the effect of each componenet of B5 and MS is dependent on the growth phase of the roots. Solasodine production increased with a decrease in total nitrogen. The ratio between ammonium nitrate and potassium nitrate was crucial. Phosphate concentration greatly influences growth, although it showed no significant affect on solasodine production. Half strength of magnesium sulphate and calcium chloride as in MS medium increased growth and solasodine production as compared to the control. This combination of the major salts with minor salts from the MS medium and chelated iron and vitamins from Gamborg's B5 medium was found to be most suitable. Hence it was possible to enhance both the specific and volumetric yield of solasodine by formulating a medium that maximized secondary metabolite production and growth in hairy root cultures. The novelty of this article lies in manipulating the components in MS medium, which supported growth, and Gamborg's B5 medium, which supported secondary product formation in hairy root cultures of S. khasianum and combining the two for maximizing both the parameters through out the growth phase. Thus it shall find immense practical applicability in hairy root scale up, where the secondary metabolite production is directly related to growth.

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“…Three years after that, James F. Bonner (1910Bonner ( -1996Fig. 86) and Robbins and White demonstrated (separately and independently) the importance of thiamine or its components thiazole and pyrimidine in root cultures (Bonner 1937;Robbins and Bartley 1937;White 1937;Gautheret 1985). An interesting sidelight to this reported by Professor Frank B. Salisbury in a biography of James Bonner is quoted here in full: ''Phillip White had grown tomato roots through repeated transfers by adding yeast extract to a medium that contained the essential mineral nutrients and sucrose as an energy source.…”

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History of orchid propagation: a mirror of the history of biotechnology

Yam

Arditti

2009

Plant Biotechnol Rep

114

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Orchid seeds are nearly microscopic in size. Because of that, many fanciful theories were proposed for the origin of orchids. Almost 400 years separate the time when orchid seeds were seen for the first time and the development of a practical asymbiotic method for their germination. The seeds were first observed and drawn during the sixteenth century. Seedlings were first described and illustrated in 1804. The association between orchid and fungi was observed as early as 1824, while the requirement for mycorrhiza for seed germination was established in 1899. An asymbiotic method for orchid seed germination was developed in 1921. After Knudson's media B and C were formulated, orchids growing and hybridization became widespread. Hybrids which early growers may not have even imagined became possible.

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VITAMIN B₁ IN THE NUTRITION OF EXCISED TOMATO ROOTS

Cited by 46 publications

References 2 publications

Green Roots: Photosynthesis and Photoautotrophy in an Underground Plant Organ

Green Roots: Photosynthesis and Photoautotrophy in an Underground Plant Organ

Manipulation of MS and B5 components for enhancement of growth and solasodine production in hairy root cultures of Solanum khasianum Clarke

History of orchid propagation: a mirror of the history of biotechnology

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VITAMIN B1 IN THE NUTRITION OF EXCISED TOMATO ROOTS

Cited by 46 publications

References 2 publications

Green Roots: Photosynthesis and Photoautotrophy in an Underground Plant Organ

Green Roots: Photosynthesis and Photoautotrophy in an Underground Plant Organ

Manipulation of MS and B5 components for enhancement of growth and solasodine production in hairy root cultures of Solanum khasianum Clarke

History of orchid propagation: a mirror of the history of biotechnology

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VITAMIN B₁ IN THE NUTRITION OF EXCISED TOMATO ROOTS