Vitamin B<sub>6</sub>Suppresses Growth of the Feline Mammary Tumor Cell Line FRM

Shimada, Dai; Fukuda, Atsushi; Kanouchi, Hiroaki; Matsumoto, Mitsuharu; Oka, Tatsuzo

doi:10.1271/bbb.70.1038

Cited by 17 publications

(8 citation statements)

References 20 publications

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“…The present study was conducted in order to evaluate the effects of specific amino acids on feline T cells in vitro. To date, cell lines are predominantly used for infection trials and oncological research (Shimada et al 2006;Alazawy et al 2011;Boenzli et al 2011;Martin et al 2011;Mochizuki et al 2011;Robert-Tissot et al 2011). However, in vitro studies might also be interesting as screening method for immunonutritional effects in cats.…”

Section: Discussionmentioning

confidence: 99%

“…Current research with feline cell lines has focused on infection trials, especially with viruses (Boenzli et al 2011;Robert-Tissot et al 2011;Alazawy et al 2011), or investigations with tumor cells (Shimada et al 2006;Martin et al 2011;Mochizuki et al 2011). However, cell cultures can also be used to focus on physiological immune responses, for example to assess the immunmodulatory effects of nutritional factors.…”

Section: Introductionmentioning

confidence: 99%

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Effects of free amino acids on cytokine secretion and proliferative activity of feline T cells in an in vitro study using the cell line MYA-1

2016

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In vitro studies might be an interesting screening method for targeted in vivo studies in the field of immunonutrition and help to reduce and refine animal studies. As the role of amino acids for immune function of cats has not been evaluated in detail so far, the present study aimed at investigating the effects of eight different amino acids (arginine, leucine, isoleucine, valine, glutamine, lysine, threonine and tryptophan) in six concentrations each (0, 0.25, 0.5, 1, 2 and 8x the cat blood level) on cytokine secretion and proliferative activity of feline T cells (MYA-1) in vitro. The results demonstrated that high doses of arginine increased IL-4, IL-10 and TNF-a secretion of T cells, while increasing concentrations of lysine increased IL-10 secretion and proliferative activity of the T cells. High doses of leucine enhanced GM-CSF and IL-10 secretion, while concentrations of threonine in the cell culture media greater than blood concentration also increased GM-CSF and additionally TNF-a secretion of the cells. The effects of glutamine and isoleucine on T cell function were only small. In conclusion, the present in vitro study could evaluate the immunomodulating potential of specific amino acids for feline T cell function. High doses of arginine, lysine, leucine and threonine had a significant impact on cytokine secretion and proliferative activity of the T cells. Targeted in vivo studies should investigate the clinical relevance of dietary supplementation of those amino acids in healthy and diseased cats as a next step.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Effects of free amino acids on cytokine secretion and proliferative activity of feline T cells in an in vitro study using the cell line MYA-1

2016

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“…The Lu and PN ligands (despite PN is active against the hepatoma cell line HepG2 [26], the human pancreatic carcinoma cell line PANC-1 [27] and the feline mammary tumour cell line FRM [28]) showed a low activity and, as in other cases [29], the activities of the complexes decreased as the size of the R substituent decreased; the Me and Et derivatives had a low activity that made determination of the IC 50 value unwarranted.…”

Section: In Vitro Antitumor Screeningmentioning

confidence: 99%

Synthesis, structure and cytotoxicity of diorganotin(IV) complexes of 2,6-lutidine-α2,3-diol (Lu): The crystal structures of Lu and [SnMe2(H2O)(Lu-2H)]

Casas

Castiñeiras

Condori

et al. 2007

Journal of Organometallic Chemistry

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“…Analyses of PN supplementation in cancer animal models (azoxymethane-induced colon tumorigenesis and 7,12-dimethylbenzanthracene-induced mammary tumorigenesis) revealed that high-dose PN prevented tumor progression ( 14 , 15 ). In human hepatoma HepG2 cells ( 16 ) or feline mammary tumor FRM cells ( 17 ), highdose B 6 was found to inhibit cell growth and induce apoptosis. However, the mechanism of the antitumor effects of B 6 has not yet been elucidated.…”

mentioning

confidence: 99%

High Dose of Pyridoxine Induces IGFBP-3 mRNA Expression in MCF-7 Cells and Its Induction Is Inhibited by the p53-Specific Inhibitor Pifithrin-α

Nakari

Kanouchi

Oka

2011

J Nutr Sci Vitaminol

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Summary It has been reported that supplementation with high-dose vitamin B 6 (B 6 ) exerts antitumor effects in rodent models of cancer. However, the mechanism of these effects remains poorly understood. High-dose B 6 also suppresses cell proliferation and induces apoptosis of human breast adenocarcinoma MCF-7 cells. Based on preliminary experiments using DNA microarray analyses, we hypothesized that high-dose pyridoxine (PN) might induce IGF-binding protein-3 (IGFBP-3) expression in MCF-7 cells. In this study, we investigated IGFBP-3 induction by 3 or 10 m M PN using a quantitative real-time PCR method. We found that the induction reached a maximum of 24-fold with 10 m M PN for 72 h compared with non-treated cells. The induction of IGFBP-3 by PN was inhibited by a p53-specific inhibitor, pifithrin-␣ , in a dose-dependent manner, but was not affected by PD169316 (MAPK inhibitor), AS601245 (c-Jun N-terminal kinase inhibitor) or SL327 (MEK1/2 inhibitor). High-dose PN did not induce p53 mRNA expression. The IGFBP-3 induction by PN seemed to be related to p53 activation.

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Vitamin B₆Suppresses Growth of the Feline Mammary Tumor Cell Line FRM

Cited by 17 publications

References 20 publications

Effects of free amino acids on cytokine secretion and proliferative activity of feline T cells in an in vitro study using the cell line MYA-1

Effects of free amino acids on cytokine secretion and proliferative activity of feline T cells in an in vitro study using the cell line MYA-1

Synthesis, structure and cytotoxicity of diorganotin(IV) complexes of 2,6-lutidine-α2,3-diol (Lu): The crystal structures of Lu and [SnMe2(H2O)(Lu-2H)]

High Dose of Pyridoxine Induces IGFBP-3 mRNA Expression in MCF-7 Cells and Its Induction Is Inhibited by the p53-Specific Inhibitor Pifithrin-α

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Vitamin B6Suppresses Growth of the Feline Mammary Tumor Cell Line FRM

Cited by 17 publications

References 20 publications

Effects of free amino acids on cytokine secretion and proliferative activity of feline T cells in an in vitro study using the cell line MYA-1

Effects of free amino acids on cytokine secretion and proliferative activity of feline T cells in an in vitro study using the cell line MYA-1

Synthesis, structure and cytotoxicity of diorganotin(IV) complexes of 2,6-lutidine-α2,3-diol (Lu): The crystal structures of Lu and [SnMe2(H2O)(Lu-2H)]

High Dose of Pyridoxine Induces IGFBP-3 mRNA Expression in MCF-7 Cells and Its Induction Is Inhibited by the p53-Specific Inhibitor Pifithrin-α

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Vitamin B₆Suppresses Growth of the Feline Mammary Tumor Cell Line FRM