Vitamin B7 protects DNA damage and stabilizes mitochondrial transmembrane potential from cryoinjury

Ranjan, Ravi; Kumar, Mahesh; Swain, Dilip Kumar; Singh, S. P.; Kharche, S. D.; Chauhan, M. S.

doi:10.1016/j.smallrumres.2022.106719

Cited by 4 publications

(2 citation statements)

References 35 publications

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“…We found that in a neutral environment, there were large proportions of live sperm. The HOS test was shown by Ranjan [11] to have a substantial positive correlation with sperm motility. Our findings, which are in line with the findings of this study, also revealed a substantial positive link between HOS and sperm motility, indicating that an aberrant pH of 6.06 shortens sperm life and damages the cell membrane.…”

Section: Discussionmentioning

confidence: 99%

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Effects of liquid storage of buck semen at refrigeration temperatures on sperm viability and fertility to develop ready to use goat semen diluent

Kumar,

Ranjan,

Bhardwaj

2024

J App Biol Biotech

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It is generally acknowledged that lower pH throughout liquid storage can decrease sperm motility and energy production, which may be advantageous for maintaining sperm viability during cryopreservation. Hence, the purpose of this study was to see how pH affected sperm viability, motility, membrane integrity, and oxidative changes after 5 days of storage in chemically specified extenders at refrigerator temperatures to formulate a ready-to-use goat semen diluent. Ejaculates were extended with tris-citric acid-fructose diluent with 20% or without egg yolk and stored at 5°C for 5 days. Sperm motility, live count, acrosome integrity, hypo-osmotic swelling positive spermatozoa, pH, and malondialdehyde all substantially differed significantly (P < 0.05) during different days of liquid storage. The pH of diluted semen decreased considerably (P < 0.05) from its original pH of 6.46 on 0 day to pH 6.06 on the 5th day of liquid storage at refrigeration temperature. Diluted semen at refrigeration temperature having 20% egg yolk had significantly higher (P < 0.05) seminal characteristics compared to liquid semen without egg yolk. The liquid storage of semen quality became worse day by day due to a drop in pH and egg yolks acted as a cryoprotectant against the harmful effects of chilling. Hence, 20% egg yolk may be used to enhance the keeping quality of diluted buck semen at refrigeration temperature for up to 5 days. Based on this result, we formulated a ready to use goat semen diluent for liquid semen storage at room temperature and refrigeration temperature.

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Section: Discussionmentioning

confidence: 99%

“…The pH of the solution was adjusted to 6.8. This sperm medium contains a basic extender with hen fresh egg yolk 20% (v/v) [11].…”

Section: Extender Medium Preparationmentioning

confidence: 99%

Effects of liquid storage of buck semen at refrigeration temperatures on sperm viability and fertility to develop ready to use goat semen diluent

Kumar,

Ranjan,

Bhardwaj

2024

J App Biol Biotech

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Extraction techniques for the determination of carotenoids and vitamins in food

Lopes Júnior,

Mendes,

Medeiros

et al. 2024

Reference Module in Chemistry, Molecular Sciences and Chemical Engineering

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Electrochemical Sensing for Vitamins

Yang

Deng

et al. 2022

Chemosensors

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Vitamins are essential and necessary nutrients for the human body. Rapid and accurate quantification of their levels in various samples has attracted much attention. Compared with traditional analytical methods, electrochemical techniques, with the advantages of low cost, high sensitivity, flexible detection strategies, easy integration, and miniaturization, have gradually become the main tools in vitamin detection. In this paper, the advance of electrochemical sensing of vitamins in recent years is reviewed. Firstly, the basics of different vitamins are briefly introduced. Then, the commonly-used electrodes and electrochemical methods for vitamin electrochemical detection, as well as the specific implementation strategy and performance, are described in detail. The development of miniaturization devices, especially microfluidic and microsensor devices, is also presented. Finally, the challenges faced by the electrochemical detection of vitamins are discussed, and future development is prospected.

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Vitamin B7 protects DNA damage and stabilizes mitochondrial transmembrane potential from cryoinjury

Cited by 4 publications

References 35 publications

Effects of liquid storage of buck semen at refrigeration temperatures on sperm viability and fertility to develop ready to use goat semen diluent

Effects of liquid storage of buck semen at refrigeration temperatures on sperm viability and fertility to develop ready to use goat semen diluent

Extraction techniques for the determination of carotenoids and vitamins in food

Electrochemical Sensing for Vitamins

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