“…Then, 2 × 150 bp paired-end sequencing (PE150) was performed on an Illumina Novaseq™. 21 The high-quality clean reads were filtered using Cutadapt with the following removed: (1) reads containing adapters, (2) reads containing polyA and polyG, (3) reads containing more than 5% unknown nucleotides (N), and (4) low-quality reads containing more than 20% low-quality bases. The quality-trimmed sequence reads were mapped to the human genome reference ( Homo sapiens Ensembl v96) using the HISAT2 software (version: hisat2-2.0.4).…”
Section: Methodsmentioning
confidence: 99%
“…Then, 2 × 150 bp paired-end sequencing (PE150) was performed on an Illumina Novaseq™. 21 The high-quality clean reads were filtered using Cutadapt with the following removed:…”
Bladder cancer is one of the common cancers worldwide, leading to 213,000 deaths in 2020. Patient with progression of non-muscle invasive bladder cancer to muscle-invasive disease will have poorer prognosis...
“…Then, 2 × 150 bp paired-end sequencing (PE150) was performed on an Illumina Novaseq™. 21 The high-quality clean reads were filtered using Cutadapt with the following removed: (1) reads containing adapters, (2) reads containing polyA and polyG, (3) reads containing more than 5% unknown nucleotides (N), and (4) low-quality reads containing more than 20% low-quality bases. The quality-trimmed sequence reads were mapped to the human genome reference ( Homo sapiens Ensembl v96) using the HISAT2 software (version: hisat2-2.0.4).…”
Section: Methodsmentioning
confidence: 99%
“…Then, 2 × 150 bp paired-end sequencing (PE150) was performed on an Illumina Novaseq™. 21 The high-quality clean reads were filtered using Cutadapt with the following removed:…”
Bladder cancer is one of the common cancers worldwide, leading to 213,000 deaths in 2020. Patient with progression of non-muscle invasive bladder cancer to muscle-invasive disease will have poorer prognosis...
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