Vitamin Deficiencies of Fifty Yeasts and Molds

Burkholder, Paul R.; Moyer, Dorothy

doi:10.2307/2481559

Cited by 74 publications

(19 citation statements)

References 5 publications

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“…Isolates of 2'. mentagrophytes studied by Burkeholder & Moyer (1943) and by Robbins & Ma (1945) were also auxo-autotrophic, while the strain investigated by ArfaLeg0 & Cury (1950) required added thiamine and inositol.…”

Section: P M Stockdalementioning

confidence: 96%

Requirements for the Growth and Sporulation of Trichophyton persicolor

Stockdale¹

1953

Journal of General Microbiology

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SUMMARY: The optimum temperature for the growth and sporulation of Trichophyton ~U S~C O Z O T was 25-30' ; 4 0 ' inhibited growth. A range of pH values from 4 to 10 was compatible with growth, the optimum pH value being slightly less than pH 7. Macroconidia were produced over a narrower range and were most abundant at pH 8.Percentage germination and length of germ tube were greatest at 100 yo humidity. No germination occurred at humidities of below about 95 yo. Exposure to light had no effect on growth rate, sporulation or pigmentation.The carbon compounds which supported best growth were mannose, glucose, mannitol and maltose. Nitrates were not utilized, and ammonium sulphate, urea and asparagin supported poor growth. The amino-acids leucine, glycine, histidine and cysteine were utilized; with all other single amino-acids tested, growth was negligible. Methionine and phenylalanine were inhibitory.T . persicolor was autotrophic for vitamins. It grew as well on a vitamin-free medium as on the basal medium.The elements zinc, iron, copper and manganese, as sulphates, stimulated the growth and sporulation of T . persicolor on a medium treated for the removal of trace elements.This investigation arose from an observation that two isolates of Trichophyton persicolor (M2, isolated in France, and M3, isolated in Britain) grew yery differently from each other on Sabouraud's ' conservation ' medium in which a peptic digest of meat replaced commercial peptone, while on Sabouraud's maltose ' proof' medium containing the same digest as nitrogen source their cultural appearance was similar. As no detailed investigation of the physiology of T . persicolor had been made previously it was decided to make a study of the nutritional and physico-chemical growth requirements of M2 and Ma. Other isolates of T . persicolor were frequently included for comparison. Among these were one (M 323) from Dr R. Vanbreuseghem (Institut de Mddecine Tropicale Prince-Ldopold, Anvers), four (M 330, M332, Ma38 and M335) from Dr Jacqueline Walker (London School of Hygiene and Tropical Medicine) and two (S45 and S78) recently isolated from material sent to this laboratory.No explanation of the difference between M2 and M 3 has emerged during the course of this work, but an outstanding effect of environmental conditions of the production of macroconidia has been frequently noted.

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Section: P M Stockdalementioning

confidence: 96%

Requirements for the Growth and Sporulation of Trichophyton persicolor

Stockdale¹

1953

Journal of General Microbiology

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“…The growth substance could be replaced by tryptophane. T. violaceurn, another faviform species, was found by Burkeholder & Moyer (1943) to be stimulated by thiamine. Ten strains studied by Georg (1949b) were also deficient for thiamine and were further stimulated by para-amino benzoic acid, but growth of the strain investigated by ArCa-LeBo & Cury (1950) was not affected by any of the vitamins tested including thiamine.…”

Section: P H Y L L I S M Stockdalementioning

confidence: 99%

“…Of other species of Trichophyton, T. rubrum does not require vitamins for growth (Burkeholder & Moyer, 1943;Robbins & McVeigh, 1946; ArCa-LeHo & Cury, I950), but growth is stimulated and macroconidia are found more abundantly in natural media such as beef bouillon (Sabouraud, I~I O ) , serum (Spring, 1931) and on media containing tryptose (Benham, 1948). Mosher et al (1936) reported that T. interdigitale would not grow on a basal medium containing amino-acids except in the presence of any one of the vitamins pantothenic acid, crude lactoflavin, inositol, or thiamine.…”

Section: P H Y L L I S M Stockdalementioning

confidence: 99%

Nutritional Requirements of the Dermatophytes

Stockdale

1953

Biological Reviews

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“…Another effect of yeasts on the populations of Drosophila may be due to variations in composition. There are significant variations in the vitamin composition of different species and strains (see, for example, Burkholder, 1943;Burkholder and Moyer, 1943;Rogosa, 1943;and Christophersen and Holzweissig, 1950). Wagner (1944 and1949) found that two species of Drosophila of the mulleri group differed physiologically because they varied in ability to grow and develop on media inoculated with various yeasts.…”

Section: Discussionmentioning

confidence: 99%