Vitamin E protection of cell morphology and protein thiols in rat hepatocytes treated with tert-butyl hydroperoxide

Wang, Shih-Tsung; Kuo, Jyh-Huang; Chou, Rong‐Ghi R.; Lii, Chong‐Kuei

doi:10.1016/s0378-4274(96)03793-9

Cited by 11 publications

(6 citation statements)

References 28 publications

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“…Batcioglu et al [31] reported that vitamin E had modulator effect on carbonic anhydrase enzyme activity against 7,12-DMBA-induced oxidative stress in mouse brain. Wang et al [32] reported and demonstrated that alpha-tocopherol helps protect extent of thiol modification of carbonic anhydrase III. TPM is also widely known as a carbonic anhydrase inhibitor [3,4].…”

Section: Discussionmentioning

confidence: 99%

Effects of Selenium and Topiramate on Lipid Peroxidation and Antioxidant Vitamin Levels in Blood of Pentylentetrazol-Induced Epileptic Rats

Kutluhan

Nazıroğlu

Çelik

et al. 2009

Biol Trace Elem Res

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Free radicals and selenium (Se) deficiency are involved in pathogenesis of epilepsy. Topiramate (TPM), a new anticonvulsant, was reported to possess neuroprotective effect via inhibition of free radicals. We investigated the effects of Se and TPM on pentylentetrazol (PTZ)-induced blood toxicity in rats. Forty male Wistar rats were equally divided into five groups. First and second groups were used as control and PTZ group, respectively. TPM and Se were administrated to rats constituting third and forth groups for 7 days, respectively. The TPM and Se combination were given to animals in fifth group for 7 days. At the end of 7 days all groups except the first group received single dose PTZ. The brain cortex samples were taken at 3 h of PTZ administration. PTZ resulted in significant increase in plasma and erythrocytes lipid peroxidation (LP) levels although plasma vitamin E concentrations and erythrocytes glutathione peroxidase (GSH-Px) activities were reduced by PTZ. The plasma and erythrocytes LP levels in third, fourth, and fifth groups were decreased as compared to second group although GSH-Px and reduced glutathione values increased in the groups. Vitamin C and E concentrations were increased through fourth and fifth group only. Vitamin A concentrations were not changed by PTZ. In conclusion, Se and TPM seem to have protective effects on the PTZ-induced blood toxicity by inhibiting free radical supporting antioxidant redox system.

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Section: Discussionmentioning

confidence: 99%

Effects of Selenium and Topiramate on Lipid Peroxidation and Antioxidant Vitamin Levels in Blood of Pentylentetrazol-Induced Epileptic Rats

Kutluhan

Nazıroğlu

Çelik

et al. 2009

Biol Trace Elem Res

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“…Such a presumption is based on a well known concerted action of thiols and vitamin E in the cellular antioxidant network (called ''the thiol redox cycle'') where thiols support the continuous recycling of vitamins E and C [43]. There are also reports documenting the protective effect of a-tocopherol, which helps to maintain protein thiols in the reduced state in rat hepatocytes treated with tert-butyl hydroperoxide [44]. Another finding of this study aimed at evaluating changes in the capacity of antioxidant defenses to counteract oxidative stress induced by chronic pressureoverload is that the activities of antioxidant enzymes in the hypertrophied LV tissue samples remained practically unchanged compared with the values recorded in shamoperated controls.…”

Section: Discussionmentioning

confidence: 99%

Short-term effects of electrically induced tachycardia on antioxidant defenses in the normal and hypertrophied rat left ventricle

Kłapcińska¹,

Sadowska-Krępa²,

Jagsz³

et al. 2009

J Physiol Sci

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Increased oxidative stress resulting from enhanced production of reactive oxygen species and/or inadequate mechanisms of antioxidant defenses has been recognized as an important factor contributing to the initiation and progression of cardiac dysfunction under a wide variety of pathophysiological conditions. The main objective of this study was to examine the effect of electrically induced tachycardia on oxidative stress and the capacity of antioxidant defenses in the normal and hypertrophied left ventricle (LV) in the rat. Left ventricular hypertrophy (LVH) was produced by banding the descending abdominal aorta. The activities of antioxidant enzymes, concentrations of non-enzymatic antioxidants, and biomarkers of oxidative stress were measured in the LV of aortic-banded animals (LVH), untreated or banded rats subjected to short-term (45 min) atrial pacing [(CTR + S) and (LVH + S), respectively], and untreated (CTR) or sham-operated (SHAM) controls. The results indicate that the increase in heart rate in vivo as a result of atrial pacing to a maximum level, independent of sympathetic nerve activity, leads to a substantial increase in oxidative stress and a marked decline in the activities of antioxidant enzymes in both the normal and hypertrophied left ventricle of the rat. The accompanying increase in tissue content of alpha- and gamma-tocopherols seem to contribute to attenuation of the oxidant stress-related loss of thiol stores in the LV. Stable left ventricular hypertrophy induced by aortic banding for six weeks has a minor impact on the capacity of the endogenous antioxidant defense system in the LV, but significantly and negatively affects the ability of the heart LV to tolerate the stress of tachycardia.

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“…Our previous study demonstrated that protection of cell morphology by vitamin E is associated with protein thiols [10, 11]. Vitamin E prevents the death of cultured hepatocytes treated with TBH [26, 27].…”

Section: Discussionmentioning

confidence: 99%

“…Male Sprague-Dawely rats (8 weeks) were purchased from the National Animal Breeding and Research Center, Taipei, Taiwan. Hepatocytes were isolated from the liver of these animals by collagenase perfusion [10], and >90% were found to be viable by the trypan blue exclusion test. The cells were then plated to collagen-precoated 30-mm plastic tissue culture dishes (Falcon Labware, USA) with a total of 0.7 × 10 6 cells in L-15 culture medium (pH 7.6) containing 18 mM N-2-hydroxyethylpiperazine-N′-2-ethanesulfonic acid (HEPES), 2.5% fetal bovine serum, 5 mg/L each of insulin and transferrin, 5 μ g/L sodium selenite, 1 g/L galactose, 1 μ mol/L dexamethasone, 100,000 IU/L penicillin, and 100 mg/L streptomycin.…”

Section: Methodsmentioning

confidence: 99%

“…This can result in the change in the verapamil and nifedipine-sensitive Ca 2+ channels [8] or an increased possibility of arachidonic acid-induced toxicity of CYPE1-expressing cells [9]. This increase in cytosolic Ca 2+ concentration can be prevented by vitamin E; moreover, we have also demonstrated that vitamin E may prevent bleb formation and the loss of protein thiols in tert -butyl hydroperoxide-(TBH-) treated hepatocytes [10, 11]. Since vitamin E only protects protein thiols which have been depleted due to interaction with endogenously generated lipid peroxidation products [12], it is possible that the attenuation of plasma membrane protein thiols may be related to the maintenance of intracellular calcium homeostasis.…”

Section: Introductionmentioning

confidence: 99%

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The Protection of Hepatocyte Cells from the Effects of Oxidative Stress by Treatment with Vitamin E in Conjunction with DTT

Tsai

Chen

et al. 2010

Journal of Biomedicine and Biotechnology

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We investigated the effect of vitamin E on membrane protein thiols under oxidative stress, which we induced by treating hepatocytes with tert-butyl hydroperoxide (TBH) for 60 mins. Those cells which we pretreated with vitamin E formed fewer blebs (22.3% compared to 60.0% in nonvitamin E-treated cells) and maintained cytosolic calcium concentration and the number of membrane protein thiols instead of showing the usual symptoms in cells undergoing oxidative stress. Dithiothreitol (DTT) also commonly reduces bleb formation in hepatocytes affected by TBH. However, our experiments clearly demonstrate that DTT does not prevent the changes in cytosolic calcium and membrane protein thiols in the blebbing cells. Consequently, we decided to pretreat cells with both DTT and vitamin E and found that the influence of TBH was entirely prevented. These findings may provide us with a new aspect for investigating the mechanism of bleb formation under oxidative stress.

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Vitamin E protection of cell morphology and protein thiols in rat hepatocytes treated with tert-butyl hydroperoxide

Cited by 11 publications

References 28 publications

Effects of Selenium and Topiramate on Lipid Peroxidation and Antioxidant Vitamin Levels in Blood of Pentylentetrazol-Induced Epileptic Rats

Effects of Selenium and Topiramate on Lipid Peroxidation and Antioxidant Vitamin Levels in Blood of Pentylentetrazol-Induced Epileptic Rats

Short-term effects of electrically induced tachycardia on antioxidant defenses in the normal and hypertrophied rat left ventricle

The Protection of Hepatocyte Cells from the Effects of Oxidative Stress by Treatment with Vitamin E in Conjunction with DTT

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