Vitellin of Pteromalus puparum (Hymenoptera: Pteromalidae), a pupal endoparasitoid of Pieris rapae (Lepidoptera: Pieridae): Biochemical characterization, temporal patterns of production and degradation

Dong, Shao-Jing; Ye, Gong Yin; Zhu, Jiachen; Chen, Zheng Xian; Hu, Cui; Liu, Shu‐Sheng

doi:10.1016/j.jinsphys.2007.01.008

Cited by 21 publications

(44 citation statements)

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“…In our previous studies, it is evident that vitellogenesis of P. puparum is initiated after the pupal stage and completed at the adult stage 48 h after eclosion (Dong et al 2007), and adult nutrition significantly affects ovarian development (Dong et al 2008). In addition, biochemical characterization of vitellogenin or vitellin (Dong et al 2007), cloning of the vitellogenin gene (Ye et al 2008), the process of vitellogenesis and its endocrine regulation (Dong et al 2007, 2009), and oogenesis and programmed cell death of nurse cells (Dong et al 2010) have been well studied. However, events and mechanisms during the following adult stage, especially when P. puparum face poor environmental conditions, are not known clearly.…”

Section: Introductionmentioning

confidence: 99%

Oosorption in the Endoparasitoid,Pteromalus puparum

Guo

Dong

et al. 2011

Journal of Insect Science

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Oosorption is the resorption of oocytes in the ovaries, and is usually induced by environmental stress. It has been demonstrated in some insect species, but overall the mechanisms of oosorption are poorly understood. In this study, the oosorption in the endoparasitic wasp, Pteromalus puparum L. (Hymenoptera: Pteromalidae), was observed in response to starvation. To explore the details of oosorption in P. puparum, both levels of hemolymph vitellogenin and ovarian vitellin were determined using sandwich ELISA. The results indicated that both levels of vitellin and total protein in the ovaries were significantly decreased 48 h after eclosion in starved P. puparum, while those of vitellogenin and total protein in the hemolymph were increased. In addition, observation of the ultrastructure of mature oocytes in the ovarioles revealed changes in yolk protein content. Those protein yolk spheres and lipid yolk spheres that had accumulated in the oocytes, were transferred out of the oocytes of starved females. It was assumed that once oosorption was induced in P. puparum, vitellin in the oocytes was transported outside and released into the hemolymph. This information helps to elucidate a mechanism of oosorption in insects.

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Section: Introductionmentioning

confidence: 99%

Oosorption in the Endoparasitoid,Pteromalus puparum

Guo

Dong

et al. 2011

Journal of Insect Science

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“…During vitellogenesis, the fat body produces mostly yolk protein precursors which are secreted into the haemolymph and accumulate in the developing oocytes during oogenesis19212223. Because vitellogenin synthesis is positively correlated with egg maturation, embryonic growth and oviposition capacity242526, the quantity of vitellogenin is usually used as a critical role in reproductive assessment2728293031. Therefore, determining the expression levels of vitellogenin is one approach for elucidating changes in insect reproduction.…”

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confidence: 99%

Incomplete removal of Wolbachia with tetracycline has two-edged reproductive effects in the thelytokous wasp Encarsia formosa (Hymenoptera: Aphelinidae)

Wang

Jiang

et al. 2017

Sci Rep

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Wolbachia pipientis are intracellular endosymbionts that induce parthenogenesis in the parasitoid Encarsia formosa. Previous studies that focused on effects of Wolbachia on the wasp usually used tetracycline to remove Wolbachia without concern for the joint influences of tetracycline and Wolbachia. Here we treated the wasps (F0 lines) with tetracycline to produce offspring (F1 lines) which were not fed tetracycline to avoid antibiotic influence. The quantitative data and fluorescence in situ hybridization showed that Wolbachia titers were reduced but not totally removed. The Wolbachia that infected the male offspring were unpredictably detected. Low dose tetracycline enhanced the fertility of 2-day-old F0 wasps after 24 h of treatment; however, compared with controls, the oocyte load of 3- to 6-day-old tetracycline-treated wasps decreased day by day, and tetracycline reduced the longevity of the wasps. The fecundity of controls was significantly higher than that of the treated F1-10 and F1-20 generations. Gene expression of vitellogenin reflected the same trend as that of wasp fecundities in both F0 and F1 lines. Moreover, female offspring proportions of F0 and F1 lines were related to the titer of infected Wolbachia, demonstrating that Wolbachia titer affected the sex determination of E. formosa.

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“…Polyvinyl 96-well microplates (NUNC, Roskilde, Denmark) were coated overnight at 4 o C with purified monoclonal antibodies against N. vitripennis Vt as the primary antibody (Dong et al, 2008), which was developed following the method of Dong et al (2007) with N. vitripennis egg extractions. Wells were washed three times with TBST (20 mmol/L Tris, pH 7.5; 150 mmol/L NaCl; 0.05% Tween-20) and blocked by incubating with 1% bovine serum albumin (BSA) for 0.5 h at 37 o C. After washing with TBST, a dilution series of purified parasitoid Vt was applied to the wells and samples were incubated in the coated plates for 1.5 h followed by three washings with TBST.…”

Section: Indirect Double Antibody Sandwich Elisamentioning

confidence: 99%

Effects of host (Boettcherisca peregrina) copper exposure on development, reproduction and vitellogenesis of the ectoparasitic wasp, Nasonia vitripennis

Dong

et al. 2009

Insect Science

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Effects of copper (Cu) accumulation by the flesh fly Boettcherisca peregrina (R.-D.) (Diptera: Sarcophagidae) on the ectoparasitic wasp Nasonia vitripennis (Walker) (Hymenoptera: Pteromalidae) were investigated experimentally by exposing host larvae to contaminated diets with final Cu concentrations of 400 +g/g and 800 +g/g diet fresh weight (DFW), respectively. Results showed that Cu can be transferred along food chains to secondary consumers (parasitoids) in small amounts, resulting in negative effects on parasitoid growth and development (body weight and developmental duration) as well as fecundity (number of offspring per female). Copper exposure also inhibited vitellogenesis of parasitoids from Cu-contaminated host pupae. It is suggested that the decreased fecundity and inhibition of vitellogenesis of N. vitripennis resulted from poor host nutritional state rather than from direct effects of Cu stress.

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Vitellin of Pteromalus puparum (Hymenoptera: Pteromalidae), a pupal endoparasitoid of Pieris rapae (Lepidoptera: Pieridae): Biochemical characterization, temporal patterns of production and degradation

Cited by 21 publications

References 50 publications

Oosorption in the Endoparasitoid,Pteromalus puparum

Oosorption in the Endoparasitoid,Pteromalus puparum

Incomplete removal of Wolbachia with tetracycline has two-edged reproductive effects in the thelytokous wasp Encarsia formosa (Hymenoptera: Aphelinidae)

Effects of host (Boettcherisca peregrina) copper exposure on development, reproduction and vitellogenesis of the ectoparasitic wasp, Nasonia vitripennis

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